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BTF3 Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS205258

Basic transcription factor 3, also known as BTF3, forms a stable complex with RNA polymerase II. This protein is required for the initiation of transcription. Recombinant human BTF3 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified… More details

$230 / 0.004 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
BTF3, Recombinant Protein
Full Product Name
BTF3, 1-209aa, Human, His tag, E Coli
Product Gene Name
Product Synonym Names
Transcription factor BTF3; BETA-NAC; BTF3a; BTF3b; NACB
Sequence
MGSSHHHHHH SSGLVPRGSH MGSMRRTGAP AQADSRGRGR ARGGCPGGEA TLSQPPPRGG TRGQEPQMKE TIMNQEKLAK LQAQVRIGGK GTARRKKKVV HRTATADDKK LQFSLKKLGV NNISGIEEVN MFTNQGTVIH FNNPKVQASL AANTFTITGH AETKQLTEML PSILNQLGAD SLTSLRRLAE ALPKQSVDGK APLATGEDDD DEVPDLVENF DEASKNEAN
Host
E Coli
Form/Format
Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.2M NaCl, 50% glycerol, 2mM DTT
Purity/Purification
> 85% by SDS-PAGE
Concentration
0.25 mg/ml (determined by Bradford assay) (lot specific)
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
24.6 kDa (229aa), confirmed by MALDI-TOF
OMIM
X53280 mRNA
3D Structure
ModBase 3D Structure for P20290
Antigen Species
Human
Tag Information
His-tag

Product Categories/Family for BTF3 recombinant protein

Applications Tested/Suitable for BTF3 recombinant protein
SDS-PAGE

Product References and Citations for BTF3 recombinant protein

Burkard T.R., et al. (2011) BMC Syst. Biol. 5:17-17; Matsuoka S., et al. (2007) Science. 316:1160-1166;

Other Notes

Small volumes of BTF3 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for BTF3 purchase

MBS205258 is a recombinant protein targeting BTF3. Also known as BTF3, 1-209aa, Human, His tag, E Coli. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for BTF3. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
transcription factor BTF3 isoform A
NCBI Official Synonym Full Names
basic transcription factor 3
NCBI Official Symbol
BTF3
NCBI Official Synonym Symbols
NACB; BTF3a; BTF3b; BETA-NAC
UniProt Protein Name
Transcription factor BTF3
UniProt Synonym Protein Names
Nascent polypeptide-associated complex subunit beta; NAC-beta; RNA polymerase B transcription factor 3
UniProt Gene Name
BTF3
UniProt Synonym Gene Names
NACB; NAC-beta
NCBI Protein Information
transcription factor BTF3

NCBI Summary for BTF3

This gene encodes the basic transcription factor 3. This protein forms a stable complex with RNA polymerase IIB and is required for transcriptional initiation. Alternative splicing results in multiple transcript variants encoding different isoforms. This gene has multiple pseudogenes. [provided by RefSeq, Jul 2008]
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UniProt Comments for BTF3

When associated with NACA, prevents inappropriate targeting of non-secretory polypeptides to the endoplasmic reticulum (ER). Binds to nascent polypeptide chains as they emerge from the ribosome and blocks their interaction with the signal recognition particle (SRP), which normally targets nascent secretory peptides to the ER. BTF3 is also a general transcription factor that can form a stable complex with RNA polymerase II. Required for the initiation of transcription.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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