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ZWINT Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS205745

ZW10 interactor isoform b, also known as ZWINT, is clearly involved in kinetochore function. Localized to the cytoplasm during interphase and to kinetochores from late prophase to anaphase, ZWINT interacts with ZW10 (Zeste White 10) and functions to regulate… More details

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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
ZWINT, Recombinant Protein
Full Product Name
ZWINT, 1-230aa, Human, His tag, E Coli
Product Gene Name
Product Synonym Names
ZW10 interactor isoform b; HZwint-1; KNTC2AP; ZWINT1
Sequence
MGSSHHHHHH SSGLVPRGSH MGSMEAAETE AEAAALEVLA EVAGILEPVG LQEEAELPAK ILVEFVVDSQ KKDKLLCSQL QVADFLQNIL AQEDTAKGLD PLASEDTSRQ KAIAAKEQWK ELKATYREHV EAIKIGLTKA LTQMEEAQRK RTQLREAFEQ LQAKKQMAME KRRAVQNQWQ LQQEKHLQHL AEVSAEGKLL FPEAEAEAEN LPDDKPQQPT RPQEQSTGDT MGRDPGVSFK AVGLQPAGDV NLP
Host
E Coli
Form/Format
Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT
Purity/Purification
> 85% by SDS-PAGE
Concentration
0.25 mg/ml (determined by Bradford assay) (lot specific)
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
27.9 kDa (253aa) confirmed by MALDI-TOF (Molecular size on SDS-PAGE will appear higher)
OMIM
AF067656 mRNA
3D Structure
ModBase 3D Structure for O95229
Antigen Species
Human
Tag Information
His-tag

Product Categories/Family for ZWINT recombinant protein

Applications Tested/Suitable for ZWINT recombinant protein
SDS-PAGE

Product References and Citations for ZWINT recombinant protein

Musio A., et al. (2004) Gene. 331: 33-40.; Lin Y T., et al. (2006) Oncogene. 25: 6901-6914.;

Other Notes

Small volumes of ZWINT recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ZWINT purchase

MBS205745 is a recombinant protein targeting ZWINT. Also known as ZWINT, 1-230aa, Human, His tag, E Coli. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for ZWINT. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
ZW10 interactor isoform b
NCBI Official Synonym Full Names
ZW10 interacting kinetochore protein
NCBI Official Symbol
ZWINT
NCBI Official Synonym Symbols
SIP30; ZWINT1; KNTC2AP; HZwint-1
UniProt Protein Name
ZW10 interactor
UniProt Synonym Protein Names
ZW10-interacting protein 1; Zwint-1
Protein Family
UniProt Gene Name
ZWINT
UniProt Synonym Gene Names
Zwint-1
NCBI Protein Information
ZW10 interactor

NCBI Summary for ZWINT

This gene encodes a protein that is clearly involved in kinetochore function although an exact role is not known. It interacts with ZW10, another kinetochore protein, possibly regulating the association between ZW10 and kinetochores. The encoded protein localizes to prophase kinetochores before ZW10 does and it remains detectable on the kinetochore until late anaphase. It has a uniform distribution in the cytoplasm of interphase cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
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UniProt Comments for ZWINT

Part of the MIS12 complex, which is required for kinetochore formation and spindle checkpoint activity. Required to target ZW10 to the kinetochore at prometaphase.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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