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ppiA Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS205981

ppiA also kmown as Peptidyl-prolyl cis-trans isomerase A. This protein is PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Recombinant E Coli ppiA protein, fused to His-tag… More details

$230 / 0.02 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
ppiA, Recombinant Protein
Full Product Name
ppiA 25-190aa, E Coli, His tag, E Coli
Product Gene Name
Product Synonym Names
Peptidyl-prolyl cis-trans isomerase A; rot; rotA
Sequence
MGSSHHHHHH SSGLVPRGSH MGSAKGDPHV LLTTSAGNIE LELDKQKAPV SVQNFVDYVN SGFYNNTTFH RVIPGFMIQG GGFTEQMQQK KPNPPIKNEA DNGLRNTRGT IAMARTADKD SATSQFFINV ADNAFLDHGQ RDFGYAVFGK VVKGMDVADK ISQVPTHDVG PYQNVPSKPV VILSAKVLP
Host
E Coli
Form/Format
Liquid, In Phosphate buffered saline (pH 7.4) containing 10% glycerol
Purity/Purification
> 95% by SDS-PAGE
Concentration
1mg/ml (determined by Bradford assay) (lot specific)
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
20.5kDa (189aa) confirmed by MALDI-TOF
3D Structure
ModBase 3D Structure for P0AFL3
Antigen Species
Human
Tag Information
His-tag

Product Categories/Family for ppiA recombinant protein

Applications Tested/Suitable for ppiA recombinant protein
SDS-PAGE

Product References and Citations for ppiA recombinant protein

Liu J., et al. (1990) Proc. Natl. Acad. Sci. u.S.A. 87:4028-4032.; Hayano T., et al. (1991) Biochemistry 30:3041-3048.

Other Notes

Small volumes of ppiA recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ppiA purchase

MBS205981 is a recombinant protein targeting ppiA. Also known as ppiA 25-190aa, E Coli, His tag, E Coli. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for ppiA. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
GenBank Nucleotide #
NCBI Official Full Name
peptidyl-prolyl cis-trans isomerase A (rotamase A)
NCBI Official Symbol
ppiA
NCBI Official Synonym Symbols
ECK3351; JW3326; rot; rotA
UniProt Protein Name
Peptidyl-prolyl cis-trans isomerase A
UniProt Synonym Protein Names
Cyclophilin A; Rotamase A
UniProt Gene Name
ppiA
UniProt Synonym Gene Names
rot; rotA; PPIase A
NCBI Protein Information
peptidyl-prolyl cis-trans isomerase A (rotamase A)

NCBI Summary for ppiA

An fkpA, surA, ppiB, ppiD quadruple mutant is viable. [More information is available at EcoGene: EG10757]. PpiA is a peptidyl-prolyl cis-trans-isomerase (PPIase), catalyzing the conformational isomerization of prolyl residues in peptides. [More information is available at EcoCyc: EG10757].

UniProt Comments for ppiA

PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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