GOT2 Active Protein

Name: GOT2 Active Protein; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS206319
Price: 180 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS206319

GOT2, also known as glutamate oxaloacetate transaminase 2, belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. glutamate oxaloacetate transaminas is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and… More details

$180 / 0.004 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

GOT2, Active Protein

Full Product Name

GOT2, 30-430aa, Human, His tag, E Coli (Bioactivity Validated)

Product Gene Name

GOT2 active protein

Product Synonym Names

Aspartate aminotransferase 2 mitochondrial; KAT4; KATIV; mitAAT

Sequence

MGSSHHHHHH SSGLVPRGSH MGSSSWWTHV EMGPPDPILG VTEAFKRDTN SKKMNLGVGA YRDDNGKPYV LPSVRKAEAQ IAAKNLDKEY LPIGGLAEFC KASAELALGE NSEVLKSGRF VTVQTISGTG ALRIGASFLQ RFFKFSRDVF LPKPTWGNHT PIFRDAGMQL QGYRYYDPKT CGFDFTGAVE DISKIPEQSV LLLHACAHNP TGVDPRPEQW KEIATVVKKR NLFAFFDMAY QGFASGDGDK DAWAVRHFIE QGINVCLCQS YAKNMGLYGE RVGAFTMVCK DADEAKRVES QLKILIRPMY SNPPLNGARI AAAILNTPDL RKQWLQEVKV MADRIIGMRT QLVSNLKKEG STHNWQHITD QIGMFCFTGL KPEQVERLIK EFSIYMTKDG RISVAGVTSS NVGYLAHAIH QVTK

Host

E Coli

Form/Format

Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 0.15M NaCl

Purity/Purification

> 95% by SDS-PAGE

Concentration

0.5 mg/ml (determined by Bradford assay) (lot specific)

Preparation and Storage

Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.

Molecular Weight

47 kDa (424aa), confirmed by MALDI-TOF

OMIM

M22632 mRNA

3D Structure

ModBase 3D Structure for P00505

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Antigen Species

Human

Tag Information

His-tag

Endotoxin Level

< 1.0 EU per 1 microgram of protein (determined by LAL method)

Biological Activity

Specific activity is > 60 units/mg, and is defined as the amount of enzyme that convert 1umole of alpha-ketoglutarate to L-Glutamate per minute at pH 8.0 at 25C.

Assay

1. Prepare a 180ul assay buffer into a suitable container. The concentrations are 110mM Tris-HCl (pH8.0), 0.44mM beta-nicotinamide adenine dinucleotide, reduced form, 55mM L-aspartate, 2.75mM alpha-ketoglutaric acid, 82.5 units MDH.
2. Equilibrate to 25C and monitor at A340nm until the value is constant using a spectrophotometer.
3. Add 20ul of recombinant GOT2 protein 2ug/ml in assay buffer.
4. Mix by inversion and record the decrease at A340nm for approximately 5 minutes.

Product Categories/Family for GOT2 active protein

Metabolism

Applications Tested/Suitable for GOT2 active protein

SDS-PAGE

Product References and Citations for GOT2 active protein

Guidetti P, Amori L, et al. (2007). J Neurochem. 102 (1):103-11.; Lain B, Iriarte A, et al. (1995). J Biol Chem. 270 (42):24732-9.

Other Notes

Small volumes of GOT2 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GOT2 purchase

MBS206319 is an active protein targeting GOT2. Also known as GOT2, 30-430aa, Human, His tag, E Coli (Bioactivity Validated). Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for GOT2. It may not necessarily be applicable to this product.

NCBI GI #

73486658

NCBI GeneID

2806

NCBI Accession #

NP_002071

UniProt Primary Accession #

P00505

UniProt Secondary Accession #

Q53FL3; Q9BWA3; B4DJA6; E7ERW2

UniProt Related Accession #

P00505

GenBank Nucleotide #

NM_002080.3

NCBI Official Full Name

aspartate aminotransferase, mitochondrial isoform 1

NCBI Official Synonym Full Names

glutamic-oxaloacetic transaminase 2

NCBI Official Symbol

GOT2

NCBI Official Synonym Symbols

KAT4; KATIV; KYAT4; mitAAT

UniProt Protein Name

Aspartate aminotransferase, mitochondrial

UniProt Synonym Protein Names

Fatty acid-binding protein; FABP-1; Glutamate oxaloacetate transaminase 2; Kynurenine aminotransferase 4; Kynurenine aminotransferase IV; Kynurenine--oxoglutarate transaminase 4; Kynurenine--oxoglutarate transaminase IV; Plasma membrane-associated fatty acid-binding protein; FABPpm; Transaminase A

UniProt Gene Name

GOT2

UniProt Synonym Gene Names

mAspAT; FABP-1; FABPpm

NCBI Protein Information

aspartate aminotransferase, mitochondrial

NCBI Summary for GOT2

Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and inner-membrane mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2013]

UniProt Comments for GOT2

Catalyzes the irreversible transamination of the L-tryptophan metabolite L-kynurenine to form kynurenic acid (KA). Plays a key role in amino acid metabolism. Important for metabolite exchange between mitochondria and cytosol. Facilitates cellular uptake of long-chain free fatty acids.

Research Articles on GOT2

1. Like in adults, FABP seems to be associated with markers of metabolic risk in obese adolescents

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with GOT2 active protein

Reference Product	PubMed Publications
MDH2 active protein	>7 publications with GOT2 and MDH2
MDH1 active protein	>3 publications with GOT2 and MDH1
GPT2 active protein	>1 publications with GOT2 and GPT2
CAD active protein	>1 publications with GOT2 and CAD
GLUD1 active protein	>1 publications with GOT2 and GLUD1

Pathways associated with GOT2 active protein

Products by Pathway	Pathway Diagram
2-Oxocarboxylic Acid Metabolism Pathway antibodies	2-Oxocarboxylic Acid Metabolism Pathway Diagram
2-Oxocarboxylic Acid Metabolism Pathway antibodies	2-Oxocarboxylic Acid Metabolism Pathway Diagram
4-hydroxyproline Degradation Pathway antibodies	4-hydroxyproline Degradation Pathway Diagram
Alanine And Aspartate Metabolism Pathway antibodies	Alanine And Aspartate Metabolism Pathway Diagram
Alanine, Aspartate And Glutamate Metabolism Pathway antibodies	Alanine, Aspartate And Glutamate Metabolism Pathway Diagram
Alanine, Aspartate And Glutamate Metabolism Pathway antibodies	Alanine, Aspartate And Glutamate Metabolism Pathway Diagram
Amino Acid Metabolism Pathway antibodies	Amino Acid Metabolism Pathway Diagram
Amino Acid Synthesis And Interconversion (transamination) Pathway antibodies	Amino Acid Synthesis And Interconversion (transamination) Pathway Diagram
Arginine And Proline Metabolism Pathway antibodies	Arginine And Proline Metabolism Pathway Diagram
Arginine And Proline Metabolism Pathway antibodies	Arginine And Proline Metabolism Pathway Diagram

Diseases associated with GOT2 active protein

Disease Name	Pubmed Publications
Disease Models, Animal Antibodies	>6 publications with GOT2 and Disease Models, Animal
Nervous System Diseases Antibodies	>4 publications with GOT2 and Nervous System Diseases
Cardiovascular Diseases Antibodies	>4 publications with GOT2 and Cardiovascular Diseases
Mental Disorders Antibodies	>2 publications with GOT2 and Mental Disorders
Liver Diseases Antibodies	>2 publications with GOT2 and Liver Diseases
Fatty Liver Antibodies	>2 publications with GOT2 and Fatty Liver
Hypertension Antibodies	>2 publications with GOT2 and Hypertension
Hypertrophy Antibodies	>1 publications with GOT2 and Hypertrophy
Weight Loss Antibodies	>1 publications with GOT2 and Weight Loss
Heart Diseases Antibodies	>1 publications with GOT2 and Heart Diseases

Organs/Tissues associated with GOT2 active protein

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>10 publications with GOT2 and Blood
Liver Antibodies	>7 publications with GOT2 and Liver
Brain Antibodies	>5 publications with GOT2 and Brain
Thyroid Antibodies	>3 publications with GOT2 and Thyroid
Muscle Antibodies	>3 publications with GOT2 and Muscle
Heart Antibodies	>3 publications with GOT2 and Heart
Vascular Antibodies	>2 publications with GOT2 and Vascular
Bladder Antibodies	>2 publications with GOT2 and Bladder
Stomach Antibodies	>1 publications with GOT2 and Stomach
Intestine Antibodies	>1 publications with GOT2 and Intestine