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Beta-Lactaminase (ampC) Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS206625

AmpC, also known as Beta-lactamase, is the most widespread resistance mechanism to beta-lactam antibiotics, such as the penicillins and the cephalosporins. These antibiotics have a common element in their molecular structure: a four-atom ring known as a… More details

$210 / 0.01 mg +1 FREE 8GB USB
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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Beta-lactaminase (ampC), Active Protein
Full Product Name
Beta-lactaminase, E Coli, 20-377, His-tag, E Coli (Bioactivity Validated)
Product Gene Name
Product Synonym Names
ampC; ampA; Cephalosporinase
Sequence
MGSSHHHHHH SSGLVPRGSH MAPQQINDIV HRTITPLIEQ QKIPGMAVAV IYQGKPYYFT WGYADIAKKQ PVTQQTLFEL GSVSKTFTGV LGGDAIARGE IKLSDPTTKY WPELTAKQWN GITLLHLATY TAGGLPLQVP DEVKSSSDLL RFYQNWQPAW APGTQRLYAN SSIGLFGALA VKPSGLSFEQ AMQTRVFQPL KLNHTWINVP PAEEKNYAWG YREGKAVHVS PGALDAEAYG VKSTIEDMAR WVQSNLKPLD INEKTLQQGI QLAQSRYWQT GDMYQGLGWE MLDWPVNPDS IINGSDNKIA LAARPVKAIT PPTPAVRASW VHKTGATGGF GSYVAFIPEK ELGIVMLANK NYPNPARVDA AWQILNALQ
Host
E Coli
Form/Format
Liquid. 20mM Tris-HCl Buffer (pH 8.0) containing 10% glycerol
Purity/Purification
> 95% by SDS-PAGE
Concentration
1 mg/ml (determined by Bradford assay) (lot specific)
Preparation and Storage
Can be stored at 2°C to 8°C for one week.
For long term storage, aliquot and store at -20°C to -80°C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
41.8 kDa (379aa), confirmed by MALDI-TOF
3D Structure
ModBase 3D Structure for P00811
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Tag Information
His-tag
Domain
20-377aa
Biological Activity
Specific activity is >700 units/mg, in which One unit will hydrolyze 1.0umole of Nitrocefin per minute at pH 7.0 at 37C.

Product Categories/Family for ampC active protein

Applications Tested/Suitable for ampC active protein
SDS-PAGE, Enzyme Activity

SDS-Page of ampC active protein

3 ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
ampC Active Protein – Cat# MBS206625

Product References and Citations for ampC active protein

Zhao Z., et al. (2015) Cancer Lett. 358 (1):17-26.; Martin I., et al. (2014) Cell. 157 (2):472-85.

Other Notes

Small volumes of ampC active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ampC purchase

MBS206625 is an active protein targeting ampC. Also known as Beta-lactaminase, E Coli, 20-377, His-tag, E Coli (Bioactivity Validated). Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for ampC. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
GenBank Nucleotide #
NCBI Official Full Name
penicillin-binding protein; beta-lactamase, intrinsically weak
NCBI Official Symbol
ampC
NCBI Official Synonym Symbols
ampA; ECK4146; JW4111
UniProt Protein Name
Beta-lactamase
UniProt Synonym Protein Names
Cephalosporinase
UniProt Gene Name
ampC
UniProt Synonym Gene Names
ampA
NCBI Protein Information
penicillin-binding protein; beta-lactamase, intrinsically weak

NCBI Summary for ampC

False positive lipoprotein prediction. [More information is available at EcoGene: EG10040]. The ampC -lactamase gene encodes a serine cephalosporinase . [More information is available at EcoCyc: EG10040].

UniProt Comments for ampC

This protein is a serine beta-lactamase with a substrate specificity for cephalosporins.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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