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Poly U Binding Splicing Factor 60kDa (PUF60) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS2067932
SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment

MBS2067932 is an antibody targeting PUF60. Also known as HRP-Linked Polyclonal Antibody to Poly U Binding Splicing Factor 60kDa (PUF60). Reactive in Human, Mouse, Rat. Host: Rabbit. More details

$250 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Poly U Binding Splicing Factor 60kDa (PUF60), Polyclonal Antibody
Full Product Name
HRP-Linked Polyclonal Antibody to Poly U Binding Splicing Factor 60kDa (PUF60)
Product Gene Name
Product Synonym Names
FIR; SIAHBP1; RoBPI; Fuse Binding Protein Interacting Repressor; Siah Binding Protein 1; Pyrimidine Tract Binding Splicing Factor; Ro Ribonucleoprotein Binding Protein 1
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Concentration
500ug/ml (lot specific)
Matching Pairs
Unconjugated Antibody: PUF60 (MBS2034014)
HRP Conjugated Antibody: PUF60 (MBS2067932)
Matching Pairs
HRP Conjugated Antibody: PUF60 (MBS2067932)
Immunogen: PUF60 (MBS2034111)
Molecular Weight
55,399 Da
OMIM
AF217197 mRNA
3D Structure
ModBase 3D Structure for Q9UHX1
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Immunogen
PUF60 (Asn226~Gln549)
Conjugation
HRP
Cross Reactivity
Human
Unconjugated Antibody
The unconjugated antibody version of this item is also available as catalog #MBS2034014
ISO Certification
Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
Applications Tested/Suitable for anti-PUF60 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (ELISA)

Other Notes

Small volumes of anti-PUF60 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for PUF60. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
poly(U)-binding-splicing factor PUF60 isoform h
NCBI Official Synonym Full Names
poly(U) binding splicing factor 60
NCBI Official Symbol
PUF60
NCBI Official Synonym Symbols
FIR; VRJS; RoBPI; SIAHBP1
UniProt Protein Name
Poly(U)-binding-splicing factor PUF60
UniProt Synonym Protein Names
60 kDa poly(U)-binding-splicing factor; FUSE-binding protein-interacting repressor; FBP-interacting repressor; Ro-binding protein 1; RoBP1; Siah-binding protein 1; Siah-BP1
UniProt Gene Name
PUF60
UniProt Synonym Gene Names
FIR; ROBPI; SIAHBP1; FBP-interacting repressor; RoBP1; Siah-BP1
NCBI Protein Information
poly(U)-binding-splicing factor PUF60

NCBI Summary for PUF60

This gene encodes a nucleic acid-binding protein that plays a role in a variety of nuclear processes, including pre-mRNA splicing and transcriptional regulation. The encoded protein forms a complex with the far upstream DNA element (FUSE) and FUSE-binding protein at the myelocytomatosis oncogene (MYC) promoter. This complex represses MYC transcription through the core-TFIIH basal transcription factor. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2012]
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UniProt Comments for PUF60

FIR: fuse-binding protein-interacting repressor. Interacts with (and may activate) Ro RNPs. Forms a ternary complex with far upstream element (FUSE) and FUSE-binding protein. It can repress a c-myc reporter via the FUSE. It is also known to target transcription factor IIH and inhibit activated transcription. This gene is implicated in the xeroderma pigmentosum disorder. Two alternatively spliced isoforms have been described.

Protein type: RNA splicing; RNA-binding; Spliceosome; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 8q24.3

Cellular Component: cell junction; cell-cell adherens junction; cyclin-dependent protein kinase activating kinase holoenzyme complex; nucleoplasm

Molecular Function: identical protein binding; protein binding

Disease: Verheij Syndrome
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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