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TNF ALPHA Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS210239

MBS210239 is specific for porcine tumour necrosis factor alpha (TNF alpha). The antibody recognises both native and recombinant TNF alpha. TNF alpha is expressed by a range of cells including macrophages and monocytes, and is an important mediator of the… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
TNF ALPHA, Monoclonal Antibody
Full Product Name
MOUSE ANTI PIG TNF ALPHA:Preservative Free
Product Gene Name
Clonality
Monoclonal
Isotype
IgG1
Clone Number
6E10
Host
Mouse
Form/Format
Preservative Free
Purified IgG - liquid
Concentration
IgG concentration 0.5 mg/ml (lot specific)
Specificity
This item is specific for porcine tumour necrosis factor alpha (TNF alpha). The antibody recognises both native and recombinant TNF alpha. TNF alpha is expressed by a range of cells including macrophages and monocytes, and is an important mediator of the porcine inflammatory response. Clone 6E10 is reported to neutralise the effects of porcine TNF alpha in in vitro cytotoxicity assays using WEHI 164 clone 13 murine fibrosarcoma cells, see Su, X. et al. for details.
Preparation and Storage
Store at -20 degree C. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Molecular Weight
25,254 Da
3D Structure
ModBase 3D Structure for P23563
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Immunogen
Recombinant porcine TNF alpha
Buffer Solution||Phosphate buffered saline
Target Species
Pig
Applications Tested/Suitable for anti-TNF ALPHA antibody
ELISA (EIA), Functional Assays (FN), Western Blot (WB)

Other Notes

Small volumes of anti-TNF ALPHA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TNF ALPHA purchase

MBS210239 is an antibody targeting TNF ALPHA. Also known as MOUSE ANTI PIG TNF ALPHA:Preservative Free. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for TNF ALPHA. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
tumor necrosis factor
NCBI Official Symbol
TNF
NCBI Official Synonym Symbols
TNFa
UniProt Protein Name
Tumor necrosis factor
UniProt Synonym Protein Names
Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a
UniProt Gene Name
TNF
UniProt Synonym Gene Names
TNFA; TNFSF2; TNF-a; NTF; ICD1; ICD2
UniProt Entry Name
TNFA_PIG
NCBI Protein Information
tumor necrosis factor; TNF-a; TNF-alpha; cachectin; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; tumor necrosis factor ligand superfamily member 2; tumor-necrosis factor

UniProt Comments for TNF ALPHA

Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Organs/Tissues associated with anti-TNF ALPHA antibody

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