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CLASS II RT1B (MHC) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS210489

Mouse anti Rat MHC Class II RT1B antibody, clone OX-6 recognizes a monomorphic determinant of the rat RT1B MHC class II antigen present on B lymphocytes, dendritic cells, some macrophages and certain epithelial cells. Rat MHC Class II RT1B antibody, clone… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
MHC CLASS II RT1B, Monoclonal Antibody
Full Product Name
MOUSE ANTI RAT MHC CLASS II RT1B:Biotin
Clonality
Monoclonal
Isotype
IgG1
Clone Number
OX-6
Host
Mouse
Form/Format
Biotin
Purified IgG conjugated to Biotin - lyophilised
Preparation and Storage
Prior to reconstitution store at 4 degree C. Following reconstitution store at 4 degree C for one month or at -20 degree C for longer. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 12 months from date of reconstitution.
Reconstitution
Reconstitute with 1 ml distilled water
Perservative Stabilisers||1% Bovine Serum Albumin
The addition of 0.1% sodium azide is recommended after reconstitution.
Immunogen
Rat thymocyte membrane glycoproteins.
Fusion Partners||Spleen cells from immunised BALB/c mice were fused with cells from the NS1 mouse myeloma cell line.
Buffer Solution||Phosphate buffered saline
Target Species
Rat
Applications Tested/Suitable for anti-MHC CLASS II RT1B antibody
Flow cytometry (FC/FACS)
Application Notes for anti-MHC CLASS II RT1B antibody
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry: Maximum Dilution: Neat

Testing Data of anti-MHC CLASS II RT1B antibody

Staining of rat spleen cells with Mouse anti Rat RT1B: Alexa Fluor 488
Mouse Anti-MHC Monoclonal Antibody – Cat# MBS210489

Testing Data of anti-MHC CLASS II RT1B antibody

Staining of rat spleen cells with Mouse anti Rat RTIB: Alexa Fluor 647
Mouse Anti-MHC Monoclonal Antibody, Testing Data – Cat# MBS210489

Testing Data of anti-MHC CLASS II RT1B antibody

Rat Spleen Tissue lysate probed with Mouse anti Rat MHC Class II RT1B
Mouse Anti-MHC Monoclonal Antibody, Testing Data – Cat# MBS210489

Testing Data of anti-MHC CLASS II RT1B antibody

Staining of rat spleen cells with Mouse anti Rat RT1B: FITC (MBS211026)
Mouse Anti-MHC Monoclonal Antibody, Testing Data – Cat# MBS210489

Testing Data of anti-MHC CLASS II RT1B antibody

Staining of rat spleen cells with Mouse anti Rat RT1B: RPE (MBS213835)
Mouse Anti-MHC Monoclonal Antibody, Testing Data – Cat# MBS210489

Testing Data of anti-MHC CLASS II RT1B antibody

Staining of rat spleen cells with Mouse anti Rat RT1B: FITC (MBS211025)
Mouse Anti-MHC Monoclonal Antibody, Testing Data – Cat# MBS210489

Testing Data of anti-MHC CLASS II RT1B antibody

Staining of rat spleen cells with Mouse anti Rat RT1B (MBS213028)
Mouse Anti-MHC Monoclonal Antibody, Testing Data – Cat# MBS210489

Testing Data of anti-MHC CLASS II RT1B antibody

Staining of rat splenocytes with Mouse anti Rat MHC class II RT1B (MBS213030)
Mouse Anti-MHC Monoclonal Antibody, Testing Data – Cat# MBS210489

Other Notes

Small volumes of anti-MHC CLASS II RT1B antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MHC CLASS II RT1B purchase

MBS210489 is an antibody targeting MHC CLASS II RT1B. Also known as MOUSE ANTI RAT MHC CLASS II RT1B:Biotin. Host: Mouse.
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