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CCT ETA Antibody – Rat Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS212547

Rat anti CCTeta antibody, clone PK/16/8/a recognizes the eta polypeptide of the CCT chaperonin molecule complex.CCTη is a 543 amino acid ~60kDa molecule encoded by the CCT7 gene, a member of the TCP-1 chaperonin family (UniProt: Q2NKZ1) The intact CCT… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
CCT ETA, Monoclonal Antibody
Full Product Name
RAT ANTI CCT ETA
Product Gene Name
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
PK/16/8/a
Host
Rat
Species Reactivity
Bovine, Human, Mouse, Rabbit
Form/Format
Purified
Purified IgG - liquid
Concentration
IgG concentration 1.0 mg/ml (lot specific)
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Molecular Weight
59,443 Da
3D Structure
ModBase 3D Structure for Q2NKZ1
Perservative Stabilisers
0.09% Sodium Azide
Preparation||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Histology Positive Control Tissue
Human prostate.
Buffer Solution||Phosphate buffered saline
Applications Tested/Suitable for anti-CCT ETA antibody
Immunohistology Paraffin*, Western Blot (WB)
Application Notes for anti-CCT ETA antibody
Western Blot: This item detects a band of approximately 60kD in Hela cell lysates.
Immunohistology - Paraffin: Maximum Dilution: 1/100; Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.
Western Blotting: Maximum Dilution: 1/500

Testing Data of anti-CCT ETA antibody

Western blot analysis of HeLa whole cell lysate (1) and HeLa heat stressed whole cell lysate (2) probed with Rat anti CCT eta followed by F(ab')2 Rabbit anti Rat IgG conjugated to HRP and visualized by chemiluminescence
Rat Anti-CCT ETA Monoclonal Antibody – Cat# MBS212547

Other Notes

Small volumes of anti-CCT ETA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CCT ETA purchase

MBS212547 is an antibody targeting CCT ETA. Also known as RAT ANTI CCT ETA. Reactive in Bovine, Human, Mouse, Rabbit. Host: Rat.

Additional Details

NCBI/Uniprot data below describe general gene information for CCT ETA. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
T-complex protein 1 subunit eta
NCBI Official Synonym Full Names
chaperonin containing TCP1, subunit 7 (eta)
NCBI Official Symbol
CCT7
NCBI Official Synonym Symbols
CCT-ETA; TCP-1-ETA
UniProt Protein Name
T-complex protein 1 subunit eta
UniProt Synonym Protein Names
CCT-eta
UniProt Gene Name
CCT7
UniProt Synonym Gene Names
TCP-1-eta
UniProt Entry Name
TCPH_BOVIN
NCBI Protein Information
T-complex protein 1 subunit eta; HIV-1 Nef interacting protein

UniProt Comments for CCT ETA

CCT-eta: Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. Known to play a role, in vitro, in the folding of actin and tubulin. Belongs to the TCP-1 chaperonin family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Chaperone

Cellular Component: chaperonin-containing T-complex; microtubule; mitochondrion

Molecular Function: identical protein binding; unfolded protein binding; ATP binding

Biological Process: protein folding; binding of sperm to zona pellucida
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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