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CD223 Antibody – Rat Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS212756

Rat anti Mouse CD223 antibody, clone C9B7W recognizes murine lymphocyte activation gene-3 (LAG-3), a 70kD activation-induced cell surface molecule that is also referred to as CD223. Murine CD223 is expressed on activated CD4 positive and CD8 positive… More details

$580 / 0.25 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD223, Monoclonal Antibody
Full Product Name
RAT ANTI MOUSE CD223
Product Gene Name
Clonality
Monoclonal
Isotype
IgG1
Clone Number
C9B7W
Host
Rat
Form/Format
Purified
Purified IgG - liquid
Concentration
IgG concentration 1.0 mg/ml (lot specific)
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Molecular Weight
56,978 Da
3D Structure
ModBase 3D Structure for Q61790
Perservative Stabilisers
0.09% Sodium Azide
Preparation||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Immunogen
Murine CD223 Ig fusion protein
Fusion Partners||Cells from immunised Lewis rats were fused with cells of the Sp/20 myeloma cell line.
Buffer Solution||Phosphate buffered saline
Target Species
Mouse
Applications Tested/Suitable for anti-CD223 antibody
Flow cytometry (FC/FACS)
Application Notes for anti-CD223 antibody
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry: Minimum Dilution: 1/50; Maximum Dilution: 1/200

Testing Data of anti-CD223 antibody

Staining of CD223 transfected cells with Rat anti Mouse CD223 (MBS212757)
Rat Anti-CD223 Monoclonal Antibody – Cat# MBS212756

Testing Data of anti-CD223 antibody

Staining of CD223 transfected cells with Rat anti Mouse CD223:Alexa Fluor 647 (MBS212756A647)
Rat Anti-CD223 Monoclonal Antibody, Testing Data – Cat# MBS212756

Testing Data of anti-CD223 antibody

Staining of CD223 transfected cells with Rat anti Mouse CD223:Alexa Fluor 488 (MBS212756A488)
Rat Anti-CD223 Monoclonal Antibody, Testing Data – Cat# MBS212756

Testing Data of anti-CD223 antibody

Staining of CD223 transfected cells with Rat anti Mouse CD223
Rat Anti-CD223 Monoclonal Antibody, Testing Data – Cat# MBS212756

Testing Data of anti-CD223 antibody

Staining of CD223 transfected cells with Rat anti Mouse CD223:RPE (MBS216008)
Rat Anti-CD223 Monoclonal Antibody, Testing Data – Cat# MBS212756

Other Notes

Small volumes of anti-CD223 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD223 purchase

MBS212756 is an antibody targeting CD223. Also known as RAT ANTI MOUSE CD223. Host: Rat.

Additional Details

NCBI/Uniprot data below describe general gene information for CD223. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
lymphocyte activation gene 3 protein
NCBI Official Synonym Full Names
lymphocyte-activation gene 3
NCBI Official Symbol
Lag3
NCBI Official Synonym Symbols
Ly66; CD223; LAG-3
UniProt Protein Name
Lymphocyte activation gene 3 protein
UniProt Synonym Protein Names
CD_antigen: CD223
UniProt Gene Name
Lag3
UniProt Synonym Gene Names
LAG-3
UniProt Entry Name
LAG3_MOUSE
NCBI Protein Information
lymphocyte activation gene 3 protein

UniProt Comments for CD223

LAG3: Involved in lymphocyte activation. Binds to HLA class-II antigens.

Protein type: Membrane protein, integral

Cellular Component: membrane; integral to membrane; external side of plasma membrane

Molecular Function: transmembrane receptor activity; MHC class II protein binding

Biological Process: cell surface receptor linked signal transduction; negative regulation of interleukin-2 biosynthetic process; positive regulation of natural killer cell mediated cytotoxicity; negative regulation of T cell activation
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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