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YTHDF1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS9133307
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MBS9133307 is an antibody targeting YTHDF1. Also known as YTHDF1 Polyclonal Antibody. Host: Rabbit. More details

$225 / 0.02 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
YTHDF1, Polyclonal Antibody
Full Product Name
YTHDF1 Polyclonal Antibody
Product Gene Name
Product Synonym Names
C20orf21
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Form/Format
PBS with 0.05% proclin300,50% glycerol,pH7.3.
Purity/Purification
Affinity Purification
Preparation and Storage
Store at -20°C. Avoid freeze / thaw cycles.
Molecular Weight
Calculated: 20kDa; 60kDa
Observed: 61kDa
OMIM
AK000398 mRNA
3D Structure
ModBase 3D Structure for Q9BYJ9
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human YTHDF1 (NP_060268.2)
Cross Reactivity
Human, Mouse, Rat

Product Categories/Family for anti-YTHDF1 antibody

ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-YTHDF1 antibody
Western Blot (WB), Immunofluorescence (IF)
Application Notes for anti-YTHDF1 antibody
WB: 1:500 - 1:2000
IF: 1:50-1:200

Western Blot (WB) of anti-YTHDF1 antibody

Western blot analysis of extracts of various cell lines, using YTHDF1 antibody at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.
Rabbit Anti-YTHDF1 Polyclonal Antibody – Cat# MBS9133307

Immunofluorescence (IF) of anti-YTHDF1 antibody

Immunofluorescence analysis of L929 cells using YTHDF1 Rabbit pAb (MBS9133307) at dilution of 1:100. Blue: DAPI for nuclear staining.
Rabbit Anti-YTHDF1 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS9133307

Immunofluorescence (IF) of anti-YTHDF1 antibody

Immunofluorescence analysis of U2OS cells using YTHDF1 Rabbit pAb (MBS9133307) at dilution of 1:100. Blue: DAPI for nuclear staining.
Rabbit Anti-YTHDF1 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS9133307

Other Notes

Small volumes of anti-YTHDF1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for YTHDF1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
YTH domain-containing family protein 1
NCBI Official Synonym Full Names
YTH N6-methyladenosine RNA binding protein 1
NCBI Official Symbol
YTHDF1
NCBI Official Synonym Symbols
C20orf21
UniProt Protein Name
YTH domain-containing family protein 1
UniProt Synonym Protein Names
Dermatomyositis associated with cancer putative autoantigen 1
UniProt Gene Name
YTHDF1
UniProt Synonym Gene Names
DACA-1
NCBI Protein Information
YTH domain-containing family protein 1

UniProt Comments for YTHDF1

Specifically recognizes and binds N6-methyladenosine (m6A)-containing mRNAs, and promotes mRNA translation efficiency (PubMed:24284625, PubMed:26046440, PubMed:26318451). M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability (PubMed:24284625). Acts as a regulator of mRNA translation efficiency: promotes ribosome loading to m6A-containing mRNAs and interacts with translation initiation factors eIF3 (EIF3A or EIF3B) to facilitate translation initiation (PubMed:26046440).
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Organs/Tissues associated with anti-YTHDF1 antibody

 Organ/Tissue Name  Pubmed Publications
 Mammary Gland Antibodies  >1 publications with YTHDF1 and Mammary Gland
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