P-Selectin Glycoprotein Ligand 1 (PSGL-1) ELISA Kit for Mouse

Name: Mouse PSGL-1 ELISA Kit; P-Selectin Glycoprotein Ligand 1; MyBioSource
Brand: MyBioSource
SKU: MBS021469
Price: 675 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS021469

Background/Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated PSGL-1 concentrations in Mouse serum, plasma and other body fluids. Using Purified Mouse PSGL-1 antibody to coat Microelisa… More details

$675 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

P-Selectin Glycoprotein Ligand 1 (PSGL-1), ELISA Kit

Full Product Name

Mouse P-Selectin Glycoprotein Ligand 1 ELISA Kit

Product Gene Name

PSGL-1 elisa kit

Species Reactivity

Mouse

Assay Type

Sandwich

Samples

Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids

Detection Range

0.25ng/ml-8ng/ml.

Sensitivity

0.1ng/ml.

Specificity

No significant cross-reactivity or interference between Mouse PSGL-1 and analogues was observed.

Preparation and Storage

Store all reagents at 2-8 degree C

Inter-assay Precision

Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean x100]

Intra-assay Precision

Intra-assay CV (%) is less than 15%.

Molecular Weight

43,201 Da

Chromosome Location

Chromosome: 12; NC_000012.11 (109015671..109027670, complement). Location: 12q24

OMIM

600738

3D Structure

ModBase 3D Structure for Q14242

Intended Uses

This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated PSGL-1 concentrations in Mouse serum, plasma and other body fluids. Using Purified Mouse PSGL-1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add PSGL-1 and PSGL-1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of PSGL-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Other Notes

Small volumes of PSGL-1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PSGL-1 purchase

MBS021469 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the P-Selectin Glycoprotein Ligand 1 (PSGL-1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PSGL-1. The ELISA analytical biochemical technique of the MBS021469 kit is based on PSGL-1 antibody-PSGL-1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PSGL-1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PSGL-1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for PSGL-1. It may not necessarily be applicable to this product.

NCBI GI #

331284238

NCBI GeneID

6404

NCBI Accession #

NP_001193538.1

UniProt Primary Accession #

Q14242

UniProt Secondary Accession #

Q12775; Q6GTW7; Q8N7J7; A8K2Y0; B4DQC3; B7Z5C7; J3KMX6

UniProt Related Accession #

Q14242

GenBank Nucleotide #

NM_001206609.1

NCBI Official Full Name

P-selectin glycoprotein ligand 1 isoform 1

NCBI Official Synonym Full Names

selectin P ligand

NCBI Official Symbol

SELPLG

NCBI Official Synonym Symbols

CLA; CD162; PSGL1; PSGL-1

UniProt Protein Name

P-selectin glycoprotein ligand 1

UniProt Synonym Protein Names

Selectin P ligand

Protein Family

P-selectin glycoprotein ligand

UniProt Gene Name

SELPLG

UniProt Synonym Gene Names

PSGL-1

UniProt Entry Name

SELPL_HUMAN

NCBI Protein Information

P-selectin glycoprotein ligand 1; cutaneous lymphocyte-associated associated antigen

NCBI Summary for PSGL-1

This gene encodes a glycoprotein that functions as a high affinity counter-receptor for the cell adhesion molecules P-, E- and L- selectin expressed on myeloid cells and stimulated T lymphocytes. As such, this protein plays a critical role in leukocyte trafficking during inflammation by tethering of leukocytes to activated platelets or endothelia expressing selectins. This protein requires two post-translational modifications, tyrosine sulfation and the addition of the sialyl Lewis x tetrasaccharide (sLex) to its O-linked glycans, for its high-affinity binding activity. Aberrant expression of this gene and polymorphisms in this gene are associated with defects in the innate and adaptive immune response. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Apr 2011]

UniProt Comments for PSGL-1

SELPLG: A SLe(x)-type glycan, which through high affinity, calcium-dependent interactions with E-, P- and L-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation. PSGL1 is critical for the initial leukocyte capture.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 12q24

Cellular Component: membrane; integral to plasma membrane; integral to membrane; plasma membrane

Molecular Function: protein binding; receptor binding

Biological Process: leukocyte tethering or rolling; blood coagulation; cell adhesion; leukocyte adhesive activation; leukocyte migration

Research Articles on PSGL-1

1. The aim of the study was to investigate the degree of platelet activation in NSCLC and the roles of PSGL-1 in the activation of platelets.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with PSGL-1 elisa kit

Reference Product	PubMed Publications
SELP elisa kit	>5 publications with PSGL-1 and SELP
SYK elisa kit	>1 publications with PSGL-1 and SYK

Pathways associated with PSGL-1 elisa kit

Products by Pathway	Pathway Diagram
Cell Adhesion Molecules (CAMs) Pathway antibodies	Cell Adhesion Molecules (CAMs) Pathway Diagram
Cell Adhesion Molecules (CAMs) Pathway antibodies	Cell Adhesion Molecules (CAMs) Pathway Diagram
Cell Surface Interactions At The Vascular Wall Pathway antibodies	Cell Surface Interactions At The Vascular Wall Pathway Diagram
Hemostasis Pathway antibodies	Hemostasis Pathway Diagram
Staphylococcus Aureus Infection Pathway antibodies	Staphylococcus Aureus Infection Pathway Diagram
Staphylococcus Aureus Infection Pathway antibodies	Staphylococcus Aureus Infection Pathway Diagram
Amb2 Integrin Signaling Pathway antibodies	Amb2 Integrin Signaling Pathway Diagram

Diseases associated with PSGL-1 elisa kit

Disease Name	Pubmed Publications
Cardiovascular Diseases Antibodies	>6 publications with PSGL-1 and Cardiovascular Diseases
Atherosclerosis Antibodies	>4 publications with PSGL-1 and Atherosclerosis
Neoplasms Antibodies	>2 publications with PSGL-1 and Neoplasms
Congenital Abnormalities Antibodies	>1 publications with PSGL-1 and Congenital Abnormalities
Hypertension Antibodies	>1 publications with PSGL-1 and Hypertension
Drug Toxicity Antibodies	>1 publications with PSGL-1 and Drug Toxicity
Diabetes Mellitus, Type 2 Antibodies	>1 publications with PSGL-1 and Diabetes Mellitus, Type 2
Myocardial Infarction Antibodies	>1 publications with PSGL-1 and Myocardial Infarction
Inflammation Antibodies	>1 publications with PSGL-1 and Inflammation
Necrosis Antibodies	>1 publications with PSGL-1 and Necrosis

Organs/Tissues associated with PSGL-1 elisa kit

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>10 publications with PSGL-1 and Blood
Bone Antibodies	>2 publications with PSGL-1 and Bone
Heart Antibodies	>2 publications with PSGL-1 and Heart
Bone Marrow Antibodies	>2 publications with PSGL-1 and Bone Marrow
Placenta Antibodies	>2 publications with PSGL-1 and Placenta
Embryonic Tissue Antibodies	>2 publications with PSGL-1 and Embryonic Tissue
Lung Antibodies	>1 publications with PSGL-1 and Lung
Spleen Antibodies	>1 publications with PSGL-1 and Spleen
Brain Antibodies	>1 publications with PSGL-1 and Brain