• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

CD3 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS216379

MBS216379 is an antibody targeting CD3. Also known as MOUSE ANTI HUMAN CD3:RPE. Host: Mouse. More details

DISCONTINUED
Click here to view our similar product(s) OR click here to contact our technical support team for suitable replacement.
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Product Name
CD3, Monoclonal Antibody
Full Product Name
MOUSE ANTI HUMAN CD3:RPE
Product Gene Name
anti-CD3 antibody
Clonality
Monoclonal
Isotype
IgG1
Clone Number
UCHT1
Host
Mouse
Form/Format
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
Preparation and Storage
Store at 4 degree C. DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of reconstitution.
Molecular Weight
20,469 Da
OMIM
186740
Reconstitution
Reconstitute in 0.25 ml disilled water
Perservative Stabilisers||0.09% Sodium Azide
1% Bovine Serum Albumin
5% Sucrose
Preparation||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Target Species
Human
Applications Tested/Suitable for anti-CD3 antibody
Flow cytometry (FC/FACS)
Application Notes for anti-CD3 antibody
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood
Flow Cytometry: Maximum Dilution: Neat

Testing Data of anti-CD3 antibody

Staining of human peripheral blood lymphocytes with Mouse anti Human CD3: FITC (MBS211022)
Mouse Anti-CD3 Monoclonal Antibody – Cat# MBS216379

Testing Data of anti-CD3 antibody

Staining of human peripheral blood lymphocytes with Mouse anti Human CD3: RPE- Alexa Fluor 750 (MBS219122P750)
Mouse Anti-CD3 Monoclonal Antibody, Testing Data – Cat# MBS216379

Testing Data of anti-CD3 antibody

Immunoperoxidase staining of human tonsil cryosection using Mouse anti Human CD3 antibody, clone UCHT1 followed by horseradish peroxidase Goat anti Mouse IgG2a antibody (MBS235411) as a detection reagent. High power
Mouse Anti-CD3 Monoclonal Antibody, Testing Data – Cat# MBS216379

Testing Data of anti-CD3 antibody

Staining of human peripheral blood lymphocytes with Mouse anti Human CD3 (MBS219122)
Mouse Anti-CD3 Monoclonal Antibody, Testing Data – Cat# MBS216379

Testing Data of anti-CD3 antibody

Staining of human peripheral blood lymphocytes with Mouse anti Human CD3:Alexa Fluor 405 (MBS219122A405)
Mouse Anti-CD3 Monoclonal Antibody, Testing Data – Cat# MBS216379

Testing Data of anti-CD3 antibody

Immunoperoxidase staining of human tonsil cryosection using Mouse anti Human CD3 antibody, clone UCHT1 followed by horseradish peroxidase Goat anti Mouse IgG2a antibody (MBS235411) as a detection reagent. High power
Mouse Anti-CD3 Monoclonal Antibody, Testing Data – Cat# MBS216379

Testing Data of anti-CD3 antibody

ELISA analysis of human CD3 expression using Rat anti Human CD3ε, clone CD3-12 (MBS215051) as a capture reagent and biotinylated Mouse anti Human CD3, clone UCHT1 (MBS213023) as a detection reagent with recombinant human CD3 as antigen to produce the standard curve. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the iMark Microplate Absorbance Reader . A serum (green) sample is included undiluted
Mouse Anti-CD3 Monoclonal Antibody, Testing Data – Cat# MBS216379

Testing Data of anti-CD3 antibody

Immunoperoxidase staining of human tonsil cryosection using Mouse anti Human CD3 antibody, clone UCHT1 followed by horseradish peroxidase Goat anti Mouse IgG2a antibody (MBS235411) as a detection reagent. Low power
Mouse Anti-CD3 Monoclonal Antibody, Testing Data – Cat# MBS216379

Testing Data of anti-CD3 antibody

Staining of human peripheral blood lymphocytes with Mouse anti Human CD3:RPE (MBS213832)
Mouse Anti-CD3 Monoclonal Antibody, Testing Data – Cat# MBS216379

Testing Data of anti-CD3 antibody

Immunoperoxidase staining of human tonsil cryosection using Mouse anti Human CD3 antibody, clone UCHT1 followed by horseradish peroxidase Goat anti Mouse IgG2a antibody (MBS235411) as a detection reagent. Medium power
Mouse Anti-CD3 Monoclonal Antibody, Testing Data – Cat# MBS216379

Other Notes

Small volumes of anti-CD3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CD3. It may not necessarily be applicable to this product.

NCBI GI #
4557429
NCBI GeneID
917
NCBI Accession #
NP_000064.1
UniProt Secondary Accession #
Q2HIZ6
UniProt Related Accession #
P09693
GenBank Nucleotide #
NM_000073.2
NCBI Official Full Name
T-cell surface glycoprotein CD3 gamma chain
NCBI Official Synonym Full Names
CD3g molecule, gamma (CD3-TCR complex)
NCBI Official Symbol
CD3G
NCBI Official Synonym Symbols
T3G; IMD17; CD3-GAMMA
UniProt Protein Name
T-cell surface glycoprotein CD3 gamma chain
UniProt Synonym Protein Names
T-cell receptor T3 gamma chain; CD_antigen: CD3g
Protein Family
CD302 antigen
UniProt Gene Name
CD3G
UniProt Synonym Gene Names
T3G
UniProt Entry Name
CD3G_HUMAN
NCBI Protein Information
T-cell surface glycoprotein CD3 gamma chain; CD3g antigen, gamma polypeptide (TiT3 complex); CD3g molecule, epsilon (CD3-TCR complex); T-cell antigen receptor complex, gamma subunit of T3; T-cell receptor T3 gamma chain

NCBI Summary for CD3

The protein encoded by this gene is the CD3-gamma polypeptide, which together with CD3-epsilon, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. Defects in this gene are associated with T cell immunodeficiency. [provided by RefSeq, Jul 2008]
Show more

UniProt Comments for CD3

CD3G: The CD3 complex mediates signal transduction.

Protein type: Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 11q23

Cellular Component: T cell receptor complex; integral to plasma membrane; plasma membrane; alpha-beta T cell receptor complex

Molecular Function: receptor signaling complex scaffold activity; transmembrane receptor activity; protein heterodimerization activity; T cell receptor binding

Biological Process: regulation of immune response; protein transport; cell surface receptor linked signal transduction; T cell activation; T cell costimulation; establishment and/or maintenance of cell polarity; innate immune response; protein complex assembly; T cell receptor signaling pathway

Disease: Immunodeficiency 17
Show more

Research Articles on CD3

1. The results suggest that CD3G should be studied as a candidate gene for autoimmunity and that CD3gamma deficiency should be considered among other primary immunodeficiencies with predominantly autoimmune manifestations.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CD3 antibody

 Reference Product  PubMed Publications
 CD3D antibody  >15 publications with CD3 and CD3D
 CD3E antibody  >8 publications with CD3 and CD3E
 CD8A antibody  >4 publications with CD3 and CD8A
 LCK antibody  >4 publications with CD3 and LCK
 CD247 antibody  >4 publications with CD3 and CD247
 FYN antibody  >2 publications with CD3 and FYN
 ITK antibody  >2 publications with CD3 and ITK
 CD8B antibody  >1 publications with CD3 and CD8B
 PTPRC antibody  >1 publications with CD3 and PTPRC

Pathways associated with anti-CD3 antibody

 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 CXCR4-mediated Signaling Events Pathway antibodies  CXCR4-mediated Signaling Events Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Costimulation By The CD28 Family Pathway antibodies  Costimulation By The CD28 Family Pathway Diagram
 Downstream TCR Signaling Pathway antibodies  Downstream TCR Signaling Pathway Diagram
 Downstream Signaling In Naive CD8+ T Cells Pathway antibodies  Downstream Signaling In Naive CD8+ T Cells Pathway Diagram
 FCGR Activation Pathway antibodies  FCGR Activation Pathway Diagram
 Fcgamma Receptor (FCGR) Dependent Phagocytosis Pathway antibodies  Fcgamma Receptor (FCGR) Dependent Phagocytosis Pathway Diagram
 Generation Of Second Messenger Molecules Pathway antibodies  Generation Of Second Messenger Molecules Pathway Diagram

Diseases associated with anti-CD3 antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >2 publications with CD3 and Inflammation
 Anemia Antibodies  >1 publications with CD3 and Anemia
 Carcinoma, Hepatocellular Antibodies  >1 publications with CD3 and Carcinoma, Hepatocellular
 Liver Neoplasms Antibodies  >1 publications with CD3 and Liver Neoplasms
 Kidney Diseases Antibodies  >1 publications with CD3 and Kidney Diseases
 Neoplasms, Experimental Antibodies  >1 publications with CD3 and Neoplasms, Experimental

Organs/Tissues associated with anti-CD3 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >17 publications with CD3 and Blood
 Thymus Antibodies  >2 publications with CD3 and Thymus
 Brain Antibodies  >1 publications with CD3 and Brain
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000