• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

CD11b Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS216466

MBS216466 is an antibody targeting CD11b. Also known as MOUSE ANTI HUMAN CD11b:FITC. Host: Mouse. More details

DISCONTINUED
Click here to view our similar product(s) OR click here to contact our technical support team for suitable replacement.
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Product Name
CD11b, Monoclonal Antibody
Full Product Name
MOUSE ANTI HUMAN CD11b:FITC
Product Gene Name
anti-CD11b antibody
Clonality
Monoclonal
Isotype
IgG1
Clone Number
ICRF44
Host
Mouse
Form/Format
FITC
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Concentration
IgG concentration 0.1 mg/ml (lot specific)
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Molecular Weight
127,307 Da
OMIM
120980
Perservative Stabilisers
0.09% Sodium Azide
1% Bovine Serum Albumin
Preparation||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Immunogen
Rheumatoid synovial cells and human monocytes
Fusion Partners||Spleen cells from immunised BALB/c mice were fused with cells of the mouse Sp2/0 myeloma cell line.
Buffer Solution||Phosphate buffered saline
Target Species
Human
Applications Tested/Suitable for anti-CD11b antibody
Flow cytometry (FC/FACS)
Application Notes for anti-CD11b antibody
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood
Flow Cytometry: Minimum Dilution: Neat; Maximum Dilution: 1/10

Testing Data of anti-CD11b antibody

Staining of human peripheral blood granulocytes with Mouse anti Human CD11b:FITC (MBS211057)
Mouse Anti-CD11b Monoclonal Antibody – Cat# MBS216466

Testing Data of anti-CD11b antibody

Immunofluorescence staining of human tonsil cryosection with Mouse anti Human CD11b antibody, clone ICRF44 (MBS213083), red in A and Mousse anti Human CD21 (MBS212051), green in B. C is the merged image with nuclei counterstained blue using DAPI. medium power
Mouse Anti-CD11b Monoclonal Antibody, Testing Data – Cat# MBS216466

Testing Data of anti-CD11b antibody

Immunofluorescence staining of human tonsil cryosection with Mouse anti Human CD11b antibody, clone ICRF44 (MBS213083), red in A and Mousse anti Human CD21 (MBS212051), green in B. C is the merged image with nuclei counterstained blue using DAPI. Low power
Mouse Anti-CD11b Monoclonal Antibody, Testing Data – Cat# MBS216466

Testing Data of anti-CD11b antibody

Staining of human peripheral blood granulocytes with Mouse anti Human CD11b:Alexa Fluor 647 (MBS213083A647)
Mouse Anti-CD11b Monoclonal Antibody, Testing Data – Cat# MBS216466

Testing Data of anti-CD11b antibody

Immunoperoxidase staining of human spleen cryosection with Mouse anti Human CD11b antibody, clone ICRF44 (MBS213083) followed by the Histar detection system . Low power
Mouse Anti-CD11b Monoclonal Antibody, Testing Data – Cat# MBS216466

Testing Data of anti-CD11b antibody

Immunoperoxidase staining of human spleen cryosection with Mouse anti Human CD11b antibody, clone ICRF44 (MBS213083) followed by the Histar detection system . High power
Mouse Anti-CD11b Monoclonal Antibody, Testing Data – Cat# MBS216466

Testing Data of anti-CD11b antibody

Immunoperoxidase staining of human tonsil cryosection with Mouse anti Human CD11b antibody, clone ICRF44 (MBS213083) followed by the Histar detection system . Low power
Mouse Anti-CD11b Monoclonal Antibody, Testing Data – Cat# MBS216466

Testing Data of anti-CD11b antibody

Staining of human peripheral blood granulocytes with Mouse anti Human CD11b:Azide Free (MBS210253)
Mouse Anti-CD11b Monoclonal Antibody, Testing Data – Cat# MBS216466

Testing Data of anti-CD11b antibody

Staining of human peripheral blood granulocytes with Mouse anti Human CD11b:RPE (MBS213853)
Mouse Anti-CD11b Monoclonal Antibody, Testing Data – Cat# MBS216466

Testing Data of anti-CD11b antibody

Staining of human peripheral blood granulocytes with Mouse anti Human CD11b:Biotin (MBS210503)
Mouse Anti-CD11b Monoclonal Antibody, Testing Data – Cat# MBS216466

Other Notes

Small volumes of anti-CD11b antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CD11b. It may not necessarily be applicable to this product.

NCBI GI #
88501734
NCBI GeneID
3684
NCBI Accession #
NP_000623.2
UniProt Secondary Accession #
Q4VAK0; Q4VAK1; Q4VAK2
UniProt Related Accession #
P11215
GenBank Nucleotide #
NM_000632.3
NCBI Official Full Name
integrin alpha-M isoform 2
NCBI Official Synonym Full Names
integrin, alpha M (complement component 3 receptor 3 subunit)
NCBI Official Symbol
ITGAM
NCBI Official Synonym Symbols
CR3A; MO1A; CD11B; MAC-1; MAC1A; SLEB6
UniProt Protein Name
Integrin alpha-M
UniProt Synonym Protein Names
CD11 antigen-like family member B; CR-3 alpha chain; Cell surface glycoprotein MAC-1 subunit alpha; Leukocyte adhesion receptor MO1; Neutrophil adherence receptor; CD_antigen: CD11b
Protein Family
Integrin
UniProt Gene Name
ITGAM
UniProt Synonym Gene Names
CD11B; CR3A
UniProt Entry Name
ITAM_HUMAN
NCBI Protein Information
integrin alpha-M; CD11 antigen-like family member B; CR-3 alpha chain; antigen CD11b (p170); cell surface glycoprotein MAC-1 subunit alpha; leukocyte adhesion receptor MO1; macrophage antigen alpha polypeptide; neutrophil adherence receptor alpha-M subunit

NCBI Summary for CD11b

This gene encodes the integrin alpha M chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as macrophage receptor 1 ('Mac-1'), or inactivated-C3b (iC3b) receptor 3 ('CR3'). The alpha M beta 2 integrin is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]
Show more

UniProt Comments for CD11b

ITGAM: a single-pass type I membrane protein of the integrin alpha chain family. Implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. Also known as CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the RGD peptide in C3b. A receptor for fibrinogen, factor X and ICAM1. Heterodimer of an alpha and a beta subunit. Alpha-M associates with beta-2. Predominantly expressed in monocytes and granulocytes.

Protein type: Motility/polarity/chemotaxis; Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 16p11.2

Cellular Component: cell surface; plasma membrane; nucleus; integrin complex; external side of plasma membrane

Molecular Function: heparin binding; heparan sulfate proteoglycan binding; protein binding; opsonin binding; protein heterodimerization activity; metal ion binding; glycoprotein binding

Biological Process: integrin-mediated signaling pathway; neutrophil chemotaxis; extracellular matrix organization and biogenesis; microglia development; cellular extravasation; activated T cell proliferation; toll-like receptor signaling pathway; innate immune response; cell adhesion; blood coagulation; toll-like receptor 4 signaling pathway; leukocyte migration; leukocyte migration during inflammatory response

Disease: Systemic Lupus Erythematosus, Susceptibility To, 6
Show more

Research Articles on CD11b

1. The transcript levels of ITGAM significantly decreased for the systemic lupus erythematosus risk allele (A) relative to the non-risk allele (G), in a dose-dependent fashion.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CD11b antibody

 Reference Product  PubMed Publications
 ITGB2 antibody  >1648 publications with CD11b and ITGB2
 ICAM1 antibody  >440 publications with CD11b and ICAM1
 ICAM2 antibody  >20 publications with CD11b and ICAM2
 PLAUR antibody  >15 publications with CD11b and PLAUR
 THY1 antibody  >15 publications with CD11b and THY1
 SELP antibody  >7 publications with CD11b and SELP
 ICAM4 antibody  >6 publications with CD11b and ICAM4
 JAM3 antibody  >5 publications with CD11b and JAM3
 GP1BA antibody  >1 publications with CD11b and GP1BA

Pathways associated with anti-CD11b antibody

 Products by Pathway  Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cell Surface Interactions At The Vascular Wall Pathway antibodies  Cell Surface Interactions At The Vascular Wall Pathway Diagram
 Extracellular Matrix Organization Pathway antibodies  Extracellular Matrix Organization Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram

Diseases associated with anti-CD11b antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >1425 publications with CD11b and Inflammation
 Disease Models, Animal Antibodies  >834 publications with CD11b and Disease Models, Animal
 Necrosis Antibodies  >826 publications with CD11b and Necrosis
 Nervous System Diseases Antibodies  >637 publications with CD11b and Nervous System Diseases
 Cardiovascular Diseases Antibodies  >514 publications with CD11b and Cardiovascular Diseases
 Brain Diseases Antibodies  >434 publications with CD11b and Brain Diseases
 Neoplasms, Experimental Antibodies  >180 publications with CD11b and Neoplasms, Experimental
 Heart Diseases Antibodies  >163 publications with CD11b and Heart Diseases
 Atherosclerosis Antibodies  >126 publications with CD11b and Atherosclerosis
 Pain Antibodies  >125 publications with CD11b and Pain

Organs/Tissues associated with anti-CD11b antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >4528 publications with CD11b and Blood
 Bone Antibodies  >858 publications with CD11b and Bone
 Bone Marrow Antibodies  >798 publications with CD11b and Bone Marrow
 Brain Antibodies  >631 publications with CD11b and Brain
 Lung Antibodies  >545 publications with CD11b and Lung
 Spleen Antibodies  >519 publications with CD11b and Spleen
 Lymph Node Antibodies  >315 publications with CD11b and Lymph Node
 Nerve Antibodies  >243 publications with CD11b and Nerve
 Intestine Antibodies  >161 publications with CD11b and Intestine
 Kidney Antibodies  >141 publications with CD11b and Kidney
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000