• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

AMER1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9206701

MBS9206701 is an antibody targeting AMER1. Also known as WTX Antibody (Center). Reactive in Human, MousePredicted: Rat, Bovine, Canine, Rabbit . Host: Rabbit. WTX is involved in kidney development. More details

$240 / 0.08 mL +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
WTX (AMER1), Polyclonal Antibody
Full Product Name
WTX Antibody (Center)
Product Gene Name
anti-AMER1 antibody
Product Synonym Names
APC membrane recruitment protein 1; Amer1; Protein FAM123B; Wilms tumor gene on the X chromosome protein; AMER1; FAM123B; WTX
Clonality
Polyclonal
Isotype
Rabbit IgG
Clone Number
RB18795
Host
Rabbit
Species Reactivity
Human, Mouse
Predicted: Rat, Bovine, Canine, Rabbit
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Specificity
This WTX antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 681-708 amino acids from the Central region of human WTX.
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Antibody/Peptide Pairs
WTX peptide (MBS9226150) is used for blocking the activity of WTX antibody (MBS9206701)
Immunogen Sequence Positions
681-708
OMIM
300373
3D Structure
ModBase 3D Structure for Q5JTC6
Function
Regulator of the canonical Wnt signaling pathway. Acts by specifically binding phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2), translocating to the cell membrane and interacting with key regulators of the canonical Wnt signaling pathway, such as components of the beta-catenin destruction complex. Acts both as a positive and negative regulator of the Wnt signaling pathway, depending on the context: acts as a positive regulator by promoting LRP6 phosphorylation. Also acts as a negative regulator by acting as a scaffold protein for the beta- catenin destruction complex and promoting stabilization of Axin at the cell membrane. Promotes CTNNB1 ubiquitination and degradation. Involved in kidney development.
Cellular Location
Cytoplasm. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Nucleus. Note=Shuttles between nucleus and cytoplasm. Detected in nuclear paraspeckles that are found close to splicing speckles. Translocates to the cell membrane following binding to PtdIns(4,5)P2
Tissue Location
Detected in fetal and adult kidney, brain and spleen.

Product Categories/Family for anti-AMER1 antibody

Cancer; Developmental biology; Signal transduction
Applications Tested/Suitable for anti-AMER1 antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes for anti-AMER1 antibody
WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
E~~Use at an assay dependent concentration

Western Blot (WB) of anti-AMER1 antibody

Western blot analysis of WTX antibody (Center) (Cat. #MBS9206701) in mouse kidney tissue lysates (35ug/lane). WTX (arrow) was detected using the purified Pab.
Rabbit Anti-AMER1 Polyclonal Antibody – Cat# MBS9206701

Immunohistochemistry (IHC) of anti-AMER1 antibody

WTX Antibody (Center) (Cat. #MBS9206701) IHC analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the WTX Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Rabbit Anti-AMER1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS9206701

Flow Cytometry (FC/FACS) of anti-AMER1 antibody

WTX Antibody (Center) (Cat. #MBS9206701) IHC analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the WTX Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Rabbit Anti-AMER1 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS9206701

Product References and Citations for anti-AMER1 antibody

Rivera,M.N., Proc. Natl. Acad. Sci. U.S.A. 106 (20), 8338-8343 (2009) Fukuzawa,R., Oncogene 28 (8), 1063-1075 (2009)

Other Notes

Small volumes of anti-AMER1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for AMER1. It may not necessarily be applicable to this product.

NCBI GI #
124244056
NCBI GeneID
139285
NCBI Accession #
NP_689637.3
UniProt Primary Accession #
Q5JTC6
UniProt Secondary Accession #
Q8N885; A2IB86
UniProt Related Accession #
Q5JTC6
GenBank Nucleotide #
NM_152424.3
NCBI Official Full Name
APC membrane recruitment protein 1
NCBI Official Synonym Full Names
APC membrane recruitment protein 1
NCBI Official Symbol
AMER1
NCBI Official Synonym Symbols
WTX; OSCS; FAM123B
UniProt Protein Name
APC membrane recruitment protein 1
UniProt Synonym Protein Names
Protein FAM123B; Wilms tumor gene on the X chromosome protein
Protein Family
APC membrane recruitment protein
UniProt Gene Name
AMER1
UniProt Synonym Gene Names
FAM123B; WTX; Amer1
UniProt Entry Name
AMER1_HUMAN
NCBI Protein Information
APC membrane recruitment protein 1

NCBI Summary for AMER1

The protein encoded by this gene upregulates trancriptional activation by the Wilms tumor protein and interacts with many other proteins, including CTNNB1, APC, AXIN1, and AXIN2. Defects in this gene are a cause of osteopathia striata with cranial sclerosis (OSCS). [provided by RefSeq, May 2010]

UniProt Comments for AMER1

WTX: a peripheral membrane protein on the cytoplasmic side that shuttles between nucleus and cytoplasm. Detected in nuclear paraspeckles that are found close to splicing speckles. Enhances trancription activation by WT1. Promotes CTNNB1 ubiquitination and degradation. Antagonizes Wnt and CTNNB1 signaling. Inactivated in approximately one-third of Wilms tumors. Detected in fetal and adult kidney, brain and spleen. Involved in kidney development. Interacts with CTNNB1, AXIN1, KEAP1, APC and BTRC. Interacts with SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes containing BTRC and/or FBXW11. Identified in a complex containing CTNNB1, APC, AXIN1 and AXIN2. Two alternatively spliced human isoforms have been reported.

Chromosomal Location of Human Ortholog: Xq11.2

Cellular Component: plasma membrane; nucleus; cytosol

Molecular Function: protein binding; phosphatidylinositol-4,5-bisphosphate binding; beta-catenin binding

Biological Process: Wnt receptor signaling pathway

Disease: Osteopathia Striata With Cranial Sclerosis
Show more

Research Articles on AMER1

1. A nonsense mutation (c.1045C>T, p.Glu349*) in the WTX gene.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-AMER1 antibody

 Reference Product  PubMed Publications
 CTNNB1 antibody  >31 publications with AMER1 and CTNNB1
 WT1 antibody  >17 publications with AMER1 and WT1
 APC antibody  >7 publications with AMER1 and APC
 KEAP1 antibody  >2 publications with AMER1 and KEAP1
 AXIN1 antibody  >2 publications with AMER1 and AXIN1
 BTRC antibody  >1 publications with AMER1 and BTRC

Diseases associated with anti-AMER1 antibody

 Disease Name  Pubmed Publications
 Osteopathia striata cranial sclerosis Antibodies  >7 publications with AMER1 and Osteopathia striata cranial sclerosis
 Aneuploidy Antibodies  >3 publications with AMER1 and Aneuploidy
 Adenocarcinoma Antibodies  >3 publications with AMER1 and Adenocarcinoma
 Premature Birth Antibodies  >1 publications with AMER1 and Premature Birth
 Hyperplasia Antibodies  >1 publications with AMER1 and Hyperplasia
 Ovarian Neoplasms Antibodies  >1 publications with AMER1 and Ovarian Neoplasms
 Fetal Death Antibodies  >1 publications with AMER1 and Fetal Death
 Nervous System Malformations Antibodies  >1 publications with AMER1 and Nervous System Malformations
 Neoplasms, Experimental Antibodies  >1 publications with AMER1 and Neoplasms, Experimental

Organs/Tissues associated with anti-AMER1 antibody

 Organ/Tissue Name  Pubmed Publications
 Kidney Antibodies  >28 publications with AMER1 and Kidney
 Lung Antibodies  >2 publications with AMER1 and Lung
 Heart Antibodies  >1 publications with AMER1 and Heart
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000