Immunoperoxidase staining of cryosectioned mouse lymph node with Rat anti Mouse CD205 (MBS216692)

Immunoperoxidase staining of mouse dendritic cells with Rat anti Mouse CD205 (MBS216692)

Immunofluorescent staining of Langerhans cells in mouse skin with Rat anti Mouse CD205 (MBS216692)

Published customer image: AIRE expression in the unreverted R/-; CreERt2 thymic rudiment. 3 -4 months old R/-; CreERt2 mice were injected with 4OHT at the doses shown (A, carrier-only, B, 0.25 mg 4OHT, C, 2 mg 4OHT). Images show immunohistochemical analysis of thymi or thymic rudiments from 4OHT mice 7 weeks post-injection. Staining is shown for AIRE, counterstained for cytokeratin 8 (K8) or CD205 as shown. DAPI reveals nuclei (blue). (A) Top and bottom panels show representative images of AIRE- regions (which comprised the majority of sections), and a rare AIRE+ cell, respectively. (B) Top and middle panels show representative images of AIRE- regions and AIRE+ cells, respectively. Bottom panel shows higher power image of middle panel. Carrier-only and 0.25 mg 4OHT injected mice, n = 2; reverted mice (i.e. injected with =0.5 mg 4OHT), n>3 (shown for 2 mg injected). Scale bars 100 um except where shown.From: Jin X, Nowell CS, Ulyanchenko S, Stenhouse FH, Blackburn CC (2014) Long-Term Persistence of Functional Thymic Epithelial Progenitor Cells In Vivo under Conditions of Low FOXN1 Expression. PLoS ONE 9(12): e114842.