Rat Anti-CD253 Monoclonal Antibody – Cat# MBS219793

CD253 Antibody – Rat Monoclonal

Name: CD253 Antibody; Monoclonal; Rat; MyBioSource
Brand: MyBioSource
SKU: MBS219793
Price: 385 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS219793

MBS219793 recognises mouse CD253, also known as TNF-related apoptosis-inducing ligand or TRAIL, a member of the tumour necrosis factor ligand superfamily. When bound to the death receptors DR4 and DR5 caspase-8-dependent apoptosis is induced. However, CD253… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

CD253, Monoclonal Antibody

Full Product Name

RAT ANTI MOUSE CD253

Product Gene Name

anti-CD253 antibody

Clonality

Monoclonal

Isotype

IgG2a

Clone Number

N2B2

Host

Rat

Form/Format

Purified
Purified IgG - liquid

Concentration

IgG concentration 0.5mg/ml (lot specific)

Specificity

This item recognises mouse CD253, also known as TNF-related apoptosis-inducing ligand or TRAIL, a member of the tumour necrosis factor ligand superfamily. When bound to the death receptors DR4 and DR5 caspase-8-dependent apoptosis is induced. However, CD253 might also be bound by the decoy receptor DcR1 which cannot induce apoptosis, and DcR2 which activates NF-kappa-B.

Clone N2B2 has been reported to block NK cell cytotoxicity in vitro.

Preparation and Storage

Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

Molecular Weight

33,477 Da

3D Structure

ModBase 3D Structure for P50592

Perservative Stabilisers

0.09% Sodium Azide (NaN3)
Preparation||Purified IgG prepared by affinity chromatography

Immunogen

Mouse CD253-transfected 2PK-3 cells
Buffer Solution||Phosphate buffered saline

Target Species

Mouse

Applications Tested/Suitable for anti-CD253 antibody

Flow cytometry (FC/FACS)

Application Notes for anti-CD253 antibody

Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Flow Cytometry: Minimum Dilution: 1/5; Maximum Dilution: 1/10

Other Notes

Small volumes of anti-CD253 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD253 purchase

MBS219793 is an antibody targeting CD253. Also known as RAT ANTI MOUSE CD253. Host: Rat.

Additional Details

NCBI/Uniprot data below describe general gene information for CD253. It may not necessarily be applicable to this product.

NCBI GI #

6678431

NCBI GeneID

22035

NCBI Accession #

NP_033451.1

UniProt Primary Accession #

P50592

UniProt Related Accession #

P50592

GenBank Nucleotide #

NM_009425.2

NCBI Official Full Name

tumor necrosis factor ligand superfamily member 10

NCBI Official Synonym Full Names

tumor necrosis factor (ligand) superfamily, member 10

NCBI Official Symbol

Tnfsf10

NCBI Official Synonym Symbols

TL2; Ly81; Trail; APO-2L; AI448571; A330042I21Rik

UniProt Protein Name

Tumor necrosis factor ligand superfamily member 10

UniProt Synonym Protein Names

TNF-related apoptosis-inducing ligand; Protein TRAIL; CD_antigen: CD253

Protein Family

Tumor necrosis factor ligand superfamily

UniProt Gene Name

Tnfsf10

UniProt Synonym Gene Names

Trail; Protein TRAIL

UniProt Entry Name

TNF10_MOUSE

NCBI Protein Information

tumor necrosis factor ligand superfamily member 10; TNF-related apoptosis inducing ligand; TNF-related apoptosis-inducing ligand; TRAIL/APO2L

UniProt Comments for CD253

TRAIL: Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG. Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis. Homotrimer. Widespread; most predominant in spleen, lung and prostate. Belongs to the tumor necrosis factor family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytokine; Membrane protein, integral

Cellular Component: extracellular space; membrane; integral to membrane

Molecular Function: cytokine activity; tumor necrosis factor receptor superfamily binding; tumor necrosis factor receptor binding

Biological Process: apoptosis; positive regulation of apoptosis; positive regulation of caspase activity; immune response

Research Articles on CD253

1. TRAIL-deficiency in ApoE(-/-) mice exacerbates nephropathy and insulin resistance

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CD253 antibody

Reference Product	PubMed Publications
Tnfrsf10b antibody	>2809 publications with CD253 and Tnfrsf10b
Casp8 antibody	>1306 publications with CD253 and Casp8
Fadd antibody	>637 publications with CD253 and Fadd
Cflar antibody	>256 publications with CD253 and Cflar
Tnfrsf11b antibody	>174 publications with CD253 and Tnfrsf11b
Ifnar1 antibody	>3 publications with CD253 and Ifnar1
Prdx4 antibody	>1 publications with CD253 and Prdx4
Tnfrsf23 antibody	>1 publications with CD253 and Tnfrsf23
Ifngr1 antibody	>1 publications with CD253 and Ifngr1

Pathways associated with anti-CD253 antibody

Products by Pathway	Pathway Diagram
Apoptosis Pathway antibodies	Apoptosis Pathway Diagram
Apoptosis Pathway antibodies	Apoptosis Pathway Diagram
Apoptosis Pathway antibodies	Apoptosis Pathway Diagram
Apoptosis Pathway antibodies	Apoptosis Pathway Diagram
Apoptosis Signaling Pathway antibodies	Apoptosis Signaling Pathway Diagram
Caspase-8 Activation By Cleavage Pathway antibodies	Caspase-8 Activation By Cleavage Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Death Receptor Signalling Pathway antibodies	Death Receptor Signalling Pathway Diagram
Dimerization Of Procaspase-8 Pathway antibodies	Dimerization Of Procaspase-8 Pathway Diagram

Diseases associated with anti-CD253 antibody

Disease Name	Pubmed Publications
Necrosis Antibodies	>3873 publications with CD253 and Necrosis
Leukemia Antibodies	>474 publications with CD253 and Leukemia
Liver Diseases Antibodies	>343 publications with CD253 and Liver Diseases
Colorectal Neoplasms Antibodies	>343 publications with CD253 and Colorectal Neoplasms
Breast Neoplasms Antibodies	>321 publications with CD253 and Breast Neoplasms
Liver Neoplasms Antibodies	>298 publications with CD253 and Liver Neoplasms
Lung Neoplasms Antibodies	>293 publications with CD253 and Lung Neoplasms
Neoplasms, Experimental Antibodies	>290 publications with CD253 and Neoplasms, Experimental
Inflammation Antibodies	>276 publications with CD253 and Inflammation
Glioma Antibodies	>251 publications with CD253 and Glioma

Organs/Tissues associated with anti-CD253 antibody

Organ/Tissue Name	Pubmed Publications
Liver Antibodies	>466 publications with CD253 and Liver
Lung Antibodies	>394 publications with CD253 and Lung
Kidney Antibodies	>156 publications with CD253 and Kidney
Bone Marrow Antibodies	>114 publications with CD253 and Bone Marrow
Muscle Antibodies	>78 publications with CD253 and Muscle
Spleen Antibodies	>73 publications with CD253 and Spleen
Mixed Antibodies	>31 publications with CD253 and Mixed
Thymus Antibodies	>27 publications with CD253 and Thymus
Spinal Cord Antibodies	>12 publications with CD253 and Spinal Cord
Mammary Gland Antibodies	>7 publications with CD253 and Mammary Gland