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COLLAGEN I/III (COL1A2) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS220768

MBS220768 is an antibody targeting COL1A2. Also known as RABBIT ANTI HUMAN COLLAGEN I/III. Reactive in Human. Host: Rabbit. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
COLLAGEN I/III (COL1A2), Polyclonal Antibody
Full Product Name
RABBIT ANTI HUMAN COLLAGEN I/III
Product Gene Name
Clonality
Polyclonal
Isotype
Polyclonal IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Purified (Purified IgG - lyophilised)
Concentration
IgG concentration 0.1 mg/ml after reconstitution. (lot specific)
Specificity
COLLAGEN I/III
This item is specific for human collagen types I and III, showing minimum cross reactivity with the following:

Human collagen type IV>3%
Human collagen type V>2%

This antibody has been cross adsorbed against human plasma proteins. Human plasma proteins do not interfere with the binding of This item to collagen.
Preparation and Storage
Prior to reconstitution store at +4 degree C.
After reconstitution store at -20 degree C.
Storage in frost-free freezers is not recommended. This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 12 months from date of reconstitution.
Molecular Weight
129,314 Da
Chromosome Location
Chromosome: 7; NC_000007.13 (94023873..94060544). Location: 7q22.1
OMIM
120160
3D Structure
ModBase 3D Structure for P08123
Immunogen
Collagen types I and III from human placenta
Antiserum Preparation
Antiserum to human collagen type I and III was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Preservative Stabilizers
Mannitol, dextran and salts.
Reconstitution
Reconstitute with 0.5 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. We recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
Buffer Solution
Phosphate buffered saline
Applications Tested/Suitable for anti-COL1A2 antibody
Immunohistology - Frozen (IHC), Immunofluorescence (IF), ELISA (EIA)
Application Notes for anti-COL1A2 antibody
Immunohistology - Frozen: Max Dilution: 1/20

Immunofluorescence: Max Dilution: 1/20

ELISA: Max Dilution: 1/4000

Other Notes

Small volumes of anti-COL1A2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for COL1A2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
collagen alpha-2(I) chain
NCBI Official Synonym Full Names
collagen, type I, alpha 2
NCBI Official Symbol
COL1A2
NCBI Official Synonym Symbols
OI4
UniProt Protein Name
Collagen alpha-2(I) chain
UniProt Synonym Protein Names
Alpha-2 type I collagen
Protein Family
UniProt Gene Name
COL1A2
UniProt Entry Name
CO1A2_HUMAN
NCBI Protein Information
collagen alpha-2(I) chain; collagen alpha-2(I) chain; type I procollagen; alpha 2(I)-collagen; alpha-2 type I collagen; collagen I, alpha-2 polypeptide; collagen of skin, tendon and bone, alpha-2 chain

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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