• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

MYOSIN (MYL9) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS223520

This antibody recognizes human myosin regulatory light chain (MRLC), also known as MYL9 and LC20. Myosin is a hexamer composed of two high molecular weight heavy chains and four light chain subunits, which include two alkali light chains and two MRLCs. MRLC… More details

$890 / 0.05 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
MYOSIN (MYL9), Polyclonal Antibody
Full Product Name
RABBIT ANTI HUMAN MYOSIN REGULATORY LIGHT CHAIN
Product Gene Name
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Birds, Mammals, Mouse, Rat
Form/Format
Purified
Purified IgG - liquid
Concentration
IgG concentration 0.5 mg/ml (lot specific)
Specificity
This antibody recognizes human myosin regulatory light chain (MRLC), also known as MYL9 and LC20.
Myosin is a hexamer composed of two high molecular weight heavy chains and four light chain subunits, which include two alkali light chains and two MRLCs. MRLC functions to stabilize the myosin heavy chain, and may also modulate the availability of cross bridges to bind to actin. Phosphorylation of the MRLC by myosin light chain kinase (MLCK) is necessary for smooth muscle contraction via activating myosin ATPase.
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Molecular Weight
13,866 Da
OMIM
609905
3D Structure
ModBase 3D Structure for P24844
Perservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
A synthetic peptide corresponding to amino acids 12-27 of human Myosin Regulatory Light Chain.
Histology Positive Control Tissue||Smooth muscle
Buffer Solution||Phosphate buffered saline
Antiserum Preparation
Antisera to Human Myosin Regulatory Light Chain were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography.
Target Species
Human
Applications Tested/Suitable for anti-MYL9 antibody
ELISA (EIA), Immunohistology Paraffin*, Western Blot (WB)
Application Notes for anti-MYL9 antibody
ELISA: Minimum Dilution: 1/5000; Maximum Dilution: 1/20000;
Immunohistology - Paraffin: Maximum Dilution: 2.5ug/ml; Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Western Blotting: Minimum Dilution: 1/500; Maximum Dilution: 1/2000

Testing Data #1 of anti-MYL9 antibody

Staining of formalin fixed, paraffin embedded human colonic smooth muscle with Rabbit anti Human myosin regulatory light chain (MBS223520)
Rabbit Anti-MYL9 Polyclonal Antibody – Cat# MBS223520

Other Notes

Small volumes of anti-MYL9 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MYL9 purchase

MBS223520 is an antibody targeting MYL9. Also known as RABBIT ANTI HUMAN MYOSIN REGULATORY LIGHT CHAIN. Reactive in Birds, Mammals, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for MYL9. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
myosin regulatory light polypeptide 9 isoform a
NCBI Official Synonym Full Names
myosin, light chain 9, regulatory
NCBI Official Symbol
MYL9
NCBI Official Synonym Symbols
LC20; MLC2; MRLC1; MYRL2; MLC-2C
UniProt Protein Name
Myosin regulatory light polypeptide 9
UniProt Synonym Protein Names
20 kDa myosin light chain; LC20; MLC-2C; Myosin RLC; Myosin regulatory light chain 2, smooth muscle isoform; Myosin regulatory light chain 9; Myosin regulatory light chain MRLC1
UniProt Gene Name
MYL9
UniProt Synonym Gene Names
MLC2; MRLC1; MYRL2; LC20
UniProt Entry Name
MYL9_HUMAN
NCBI Protein Information
myosin regulatory light polypeptide 9; 20 kDa myosin light chain; myosin RLC; myosin regulatory light chain 1; myosin regulatory light chain 2, smooth muscle isoform; myosin regulatory light chain 9; myosin regulatory light chain MRLC1; myosin, light polypeptide 9, regulatory

NCBI Summary for MYL9

Myosin, a structural component of muscle, consists of two heavy chains and four light chains. The protein encoded by this gene is a myosin light chain that may regulate muscle contraction by modulating the ATPase activity of myosin heads. The encoded protein binds calcium and is activated by myosin light chain kinase. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Show more

UniProt Comments for MYL9

MRLC1: Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion. Myosin is an hexamer of 2 heavy chains and 4 light chains. Smooth muscle tissues and in some, but not all, nonmuscle cells. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Contractile; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 20q11.23

Cellular Component: stress fiber; muscle myosin complex; Z disc; cytosol

Molecular Function: structural constituent of muscle; calcium ion binding

Biological Process: axon guidance; muscle contraction; regulation of muscle contraction; ephrin receptor signaling pathway
Show more

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000