• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

Erythropoietin RECEPTOR (EPOR) Antibody – Sheep Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS224081

MBS224081 recognises Erythropoietin Receptor (EPOR), a type I transmembrane protein and member of the type I cytokine receptor family, expressed by erythroid cells and erythroid progenitor cells, which acts as a receptor for erythropoietin (EPO) to mediate… More details

DISCONTINUED
Click here to view our similar product(s) OR click here to contact our technical support team for suitable replacement.
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Product Name
ERYTHROPOIETIN RECEPTOR (EPOR), Polyclonal Antibody
Full Product Name
SHEEP ANTI HUMAN ERYTHROPOIETIN RECEPTOR
Product Gene Name
anti-EPOR antibody
Clonality
Polyclonal
Isotype
IgG
Host
Sheep
Species Reactivity
Mouse
Form/Format
Purified
Purified IgG - liquid
Concentration
IgG concentration 1.0mg/ml (lot specific)
Specificity
This item recognises Erythropoietin Receptor (EPOR), a type I transmembrane protein and member of the type I cytokine receptor family, expressed by erythroid cells and erythroid progenitor cells, which acts as a receptor for erythropoietin (EPO) to mediate erythroblast proliferation and differentiation, and maintain progenitor survival. Binding of EPO to EPOR, results in the dimerization of the EPOR and the triggering of the JAK2/STAT5 signaling cascade, and in some cell types activation of STAT1 and STAT3. Several isoforms of EPOR exist: EPOR-F, EPOR-S and EPOR-T, and a functional EPOR is found on endothelial cells and cardiomyocytes within the cardiovascular system, suggesting a role in cardiac function. This item has been shown to block Iodine125 labelled erythropoietin ligand binding using erythroleukemia cell line TF-1 with 3000 EPOR/cell in neutralization studies, see Harris, K.W.et al. for details.This item is reported as suitable for use in immunocytochemistry.This item has been reported for use in functional studies. Removal of Sodium Azide is recommended prior to use in functional assays. The recommended concentration for functional assays is 500ug/ml.
Preparation and Storage
Store at 4 degree C. DO NOT FREEZE. This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Molecular Weight
35,809 Da
OMIM
133100
Perservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Partial cDNA of the extracellular domain of human EPOR expressed as a GST fusion protein in E Coli.
Buffer Solution||Phosphate buffered saline
Antiserum Preparation
Antiserum to human EPOR was raised by repeated immunisation of sheep with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Target Species
Human
Applications Tested/Suitable for anti-EPOR antibody
ELISA (EIA), Immunoprecipitation (IP), Western Blot (WB)
Application Notes for anti-EPOR antibody
ELISA: This product is suitable for use in indirect ELISA applications.
Western Blot: This item detects a 49kDa band of EPOR-GST on SDS-PAGE.
ELISA: Minimum Dilution: 50ng/ml
Immunoprecipitation: Minimum Dilution: 10ug/ml
Western Blotting: Minimum Dilution: 10ug/ml

Other Notes

Small volumes of anti-EPOR antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for EPOR purchase

MBS224081 is an antibody targeting EPOR. Also known as SHEEP ANTI HUMAN ERYTHROPOIETIN RECEPTOR. Reactive in Mouse. Host: Sheep.

Additional Details

NCBI/Uniprot data below describe general gene information for EPOR. It may not necessarily be applicable to this product.

NCBI GI #
4503591
NCBI GeneID
2057
NCBI Accession #
NP_000112.1
UniProt Secondary Accession #
Q15443; Q2M205; B2RCG4
UniProt Related Accession #
P19235
GenBank Nucleotide #
NM_000121.3
NCBI Official Full Name
erythropoietin receptor
NCBI Official Synonym Full Names
erythropoietin receptor
NCBI Official Symbol
EPOR
NCBI Official Synonym Symbols
EPO-R
UniProt Protein Name
Erythropoietin receptor
Protein Family
Erythropoietin receptor
UniProt Gene Name
EPOR
UniProt Synonym Gene Names
EPO-R
UniProt Entry Name
EPOR_HUMAN
NCBI Protein Information
erythropoietin receptor

NCBI Summary for EPOR

This gene encodes the erythropoietin receptor which is a member of the cytokine receptor family. Upon erythropoietin binding, this receptor activates Jak2 tyrosine kinase which activates different intracellular pathways including: Ras/MAP kinase, phosphatidylinositol 3-kinase and STAT transcription factors. The stimulated erythropoietin receptor appears to have a role in erythroid cell survival. Defects in the erythropoietin receptor may produce erythroleukemia and familial erythrocytosis. Dysregulation of this gene may affect the growth of certain tumors. Alternate splicing results in multiple transcript variants.[provided by RefSeq, May 2010]
Show more

UniProt Comments for EPOR

EPOR: erythropoietin receptor: a member of the cytokine receptor family. Mediates erythropoietin-induced erythroblast proliferation, differentiation and survival. Upon EPO stimulation, EPOR dimerizes triggering the JAK2/STAT5 signaling cascade. In some cell types, can also activate STAT1 and STAT3. Forms homodimers on EPO stimulation. Tyrosine-phosphorylated EpoR may bind several SH2 domain-containing proteins including LYN, the adapter protein APS, SHP-1, SHP-2, JAK2, PI3 kinases, STAT5A/B, SOCS3, and CRKL. Defects in the erythropoietin receptor may produce erythroleukemia and familial erythrocytosis. Three alternatively-spliced isoforms have been described. Isoform EPOR-T, missing the cytoplasmic tail, acts as a dominant-negative receptor of EPOR-mediated signaling.

Protein type: Membrane protein, integral; Receptor, cytokine

Chromosomal Location of Human Ortholog: 19p13.3-p13.2

Cellular Component: integral to plasma membrane; extracellular region

Molecular Function: identical protein binding; protein binding; erythropoietin receptor activity

Biological Process: heart development; brain development; decidualization; signal transduction

Disease: Erythrocytosis, Familial, 1
Show more

Research Articles on EPOR

1. while EPO can stimulate NO production, NO in turn can regulate EPOR expression in endothelial cells during hypoxia

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-EPOR antibody

 Reference Product  PubMed Publications
 EPO antibody  >724 publications with EPOR and EPO
 JAK2 antibody  >194 publications with EPOR and JAK2
 PTPN6 antibody  >14 publications with EPOR and PTPN6
 GRB2 antibody  >10 publications with EPOR and GRB2
 SOCS3 antibody  >8 publications with EPOR and SOCS3
 SOCS2 antibody  >6 publications with EPOR and SOCS2
 LYN antibody  >6 publications with EPOR and LYN
 CRKL antibody  >6 publications with EPOR and CRKL

Pathways associated with anti-EPOR antibody

 Products by Pathway  Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 EPO Receptor Signaling Pathway antibodies  EPO Receptor Signaling Pathway Diagram
 EPO Signaling Pathway antibodies  EPO Signaling Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Jak-STAT Signaling Pathway antibodies  Jak-STAT Signaling Pathway Diagram
 Jak-STAT Signaling Pathway antibodies  Jak-STAT Signaling Pathway Diagram
 Kit Receptor Signaling Pathway antibodies  Kit Receptor Signaling Pathway Diagram
 PI3K-Akt Signaling Pathway antibodies  PI3K-Akt Signaling Pathway Diagram

Diseases associated with anti-EPOR antibody

 Disease Name  Pubmed Publications
 Anemia Antibodies  >169 publications with EPOR and Anemia
 Polycythemia Antibodies  >79 publications with EPOR and Polycythemia
 Cardiovascular Diseases Antibodies  >73 publications with EPOR and Cardiovascular Diseases
 Disease Models, Animal Antibodies  >63 publications with EPOR and Disease Models, Animal
 Kidney Diseases Antibodies  >41 publications with EPOR and Kidney Diseases
 Neoplasms, Experimental Antibodies  >32 publications with EPOR and Neoplasms, Experimental
 Breast Neoplasms Antibodies  >32 publications with EPOR and Breast Neoplasms
 Inflammation Antibodies  >31 publications with EPOR and Inflammation
 Necrosis Antibodies  >29 publications with EPOR and Necrosis
 Adenocarcinoma Antibodies  >29 publications with EPOR and Adenocarcinoma

Organs/Tissues associated with anti-EPOR antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >357 publications with EPOR and Blood
 Bone Antibodies  >141 publications with EPOR and Bone
 Brain Antibodies  >112 publications with EPOR and Brain
 Kidney Antibodies  >90 publications with EPOR and Kidney
 Liver Antibodies  >76 publications with EPOR and Liver
 Heart Antibodies  >50 publications with EPOR and Heart
 Embryonic Tissue Antibodies  >33 publications with EPOR and Embryonic Tissue
 Lung Antibodies  >27 publications with EPOR and Lung
 Eye Antibodies  >25 publications with EPOR and Eye
 Skin Antibodies  >12 publications with EPOR and Skin
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000