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ATG101 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS224305

MBS224305 recognises human Autophagy-related protein 101 (ATG101), a 218 amino acid protein belonging to the ATG101 family. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
ATG101, Polyclonal Antibody
Full Product Name
RABBIT ANTI ATG101
Product Gene Name
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Mouse, Rat
Form/Format
Purified
Purified IgG - liquid
Concentration
IgG concentration 1.0mg/ml (lot specific)
Specificity
This item recognises human Autophagy-related protein 101 (ATG101), a 218 amino acid protein belonging to the ATG101 family.
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted.
Shelf Life: 18 months from date of despatch.
Molecular Weight
25,002 Da
Perservative Stabilisers
0.02% Sodium Azide (NaN3)
Preparation||Antisera to Human ATG101 were raised by repeated immunisation of Rabbits with highly purified antigen. Purified IgG prepared from whole serum by chromatography.
Immunogen
A synthetic peptide corresponding to 16 amino acid sequence from near the centre of human ATG101
Histology Positive Control Tissue||Mouse Liver
Buffer Solution||Phosphate buffered saline.
Target Species
Human
Applications Tested/Suitable for anti-ATG101 antibody
Immunohistology Frozen, ELISA (EIA), Western Blot (WB)
Application Notes for anti-ATG101 antibody
ELISA: This product is suitable for use in indirect ELISA applications.
Western Blot: This item detects a band of approximately 29 kDa in Human liver tissue lysates.
Immunohistology - Frozen: Minimum Dilution: 5ug/ml
Western Blotting: Minimum Dilution: 1-2ug/ml

Testing Data #1 of anti-ATG101 antibody

Western blot analysis of human liver lysate probed with Rabbit anti Human ATG101 (MBS224305) at 1.0 (A) and 2.0 (B) μgml-1
Rabbit Anti-ATG101 Polyclonal Antibody – Cat# MBS224305

Testing Data #2 of anti-ATG101 antibody

Formalin fixed, paraffin embedded mouse liver stained with Rabit anti Human ATG101 (MBS224305) following heat mediated antigen retrieval using sodium citrate buffer (pH6.0)
Rabbit Anti-ATG101 Polyclonal Antibody, Testing Data #2 – Cat# MBS224305

Other Notes

Small volumes of anti-ATG101 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ATG101 purchase

MBS224305 is an antibody targeting ATG101. Also known as RABBIT ANTI ATG101. Reactive in Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for ATG101. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
autophagy-related protein 101
NCBI Official Synonym Full Names
autophagy related 101
NCBI Official Symbol
Atg101
NCBI Official Synonym Symbols
AA407441; AU022967; 9430023L20Rik
UniProt Protein Name
Autophagy-related protein 101
UniProt Gene Name
Atg101
UniProt Entry Name
ATGA1_MOUSE
NCBI Protein Information
autophagy-related protein 101

UniProt Comments for ATG101

ATG101: an protein required for autophagosome formation. Interacts with ATG13. Associates with a complex composed of ATG13, ULK1 and RB1CC1. Under starvation conditions it localizes to phagophores, which are preautophagosomal structures. Stabilizes the expression of Atg13 in the cell. Belongs to the ATG101 family.

Protein type: Autophagy

Cellular Component: cytoplasm

Molecular Function: identical protein binding; protein complex binding

Biological Process: autophagy; autophagic vacuole formation
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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