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Neurofilament H (NEFH) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS224347

Rabbit anti Bovine Neurofilament H 200kDa antibody recognizes bovine intermediate 200kDa Neurofilament H (NFH), a 1026 amino acid ~200kDa protein involved in the maintenence of neuronal integrity. Neurofilaments are a major component of the cellular… More details

$890 / 0.1 mg +1 FREE 8GB USB
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Product Overview

Online Datasheet in PDF
Product Name
NEUROFILAMENT H (Nefh), Polyclonal Antibody
Full Product Name
RABBIT ANTI BOVINE NEUROFILAMENT H 200kDa
Product Gene Name
anti-Nefh antibody
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Form/Format
Purified
Purified IgG - liquid
Concentration
IgG concentration 1.0mg/ml (lot specific)
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Molecular Weight
116,994 Da
Perservative Stabilisers
0.05% Sodium Azide (NaN3)
Histology Positive Control Tissue
Bovine or rat cerebellum
Buffer Solution||TRIS-glycine buffered saline, NaCl
Antiserum Preparation
Antiserum to bovine NFH was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Target Species
Bovine
Applications Tested/Suitable for anti-Nefh antibody
Immunohistology Paraffin*, Western Blot (WB)
Application Notes for anti-Nefh antibody
Western Blot: This item detects a band of approximately 200kDa in bovine cerebellum tissue lysates.
Immunohistology - Paraffin: Minimum Dilution: 1.0ug/ml; Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Western Blotting: Minimum Dilution: 0.1ug/ml

Other Notes

Small volumes of anti-Nefh antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Nefh purchase

MBS224347 is an antibody targeting Nefh. Also known as RABBIT ANTI BOVINE NEUROFILAMENT H 200kDa. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for Nefh. It may not necessarily be applicable to this product.

NCBI GI #
124286811
NCBI GeneID
380684
NCBI Accession #
NP_035034.2
UniProt Secondary Accession #
Q5SVF6; Q61959; A1E2H9
UniProt Related Accession #
P19246
GenBank Nucleotide #
NM_010904.3
NCBI Official Full Name
neurofilament heavy polypeptide
NCBI Official Synonym Full Names
neurofilament, heavy polypeptide
NCBI Official Symbol
Nefh
NCBI Official Synonym Symbols
Nfh; NF-H; NF200; mKIAA0845
UniProt Protein Name
Neurofilament heavy polypeptide
UniProt Synonym Protein Names
200 kDa neurofilament protein; Neurofilament triplet H protein
Protein Family
Neurofilament heavy polypeptide
UniProt Gene Name
Nefh
UniProt Synonym Gene Names
Kiaa0845; Nfh; NF-H
UniProt Entry Name
NFH_MOUSE
NCBI Protein Information
neurofilament heavy polypeptide; 200 kDa neurofilament protein; neurofilament 200kDa; neurofilament triplet H protein

UniProt Comments for Nefh

NFH: Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins. Belongs to the intermediate filament family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytoskeletal

Cellular Component: mitochondrion; axon; cytoplasm; neurofilament; intermediate filament; perikaryon; nucleus; myelin sheath

Molecular Function: protein binding, bridging; nutrient reservoir activity; structural constituent of cytoskeleton; structural molecule activity; protein kinase binding

Biological Process: ovarian follicle atresia; embryonic development; neurofilament cytoskeleton organization and biogenesis; neurofilament bundle assembly; intermediate filament cytoskeleton organization and biogenesis; intermediate filament bundle assembly; brain development; microtubule cytoskeleton organization and biogenesis; peripheral nervous system neuron axonogenesis
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Research Articles on Nefh

1. Data conclude that NF-H and NF-M C-terminal domains do not normally regulate NF transport rates as previously proposed, but instead increase the proteolytic resistance of NF, thereby stabilizing the stationary neurofilament cytoskeleton along axons.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-Nefh antibody

 Products by Pathway  Pathway Diagram
 Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies  Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
 Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies  Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram

Diseases associated with anti-Nefh antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >15 publications with Nefh and Nervous System Diseases
 Brain Diseases Antibodies  >4 publications with Nefh and Brain Diseases
 Brain Injuries Antibodies  >3 publications with Nefh and Brain Injuries
 Prenatal Injuries Antibodies  >1 publications with Nefh and Prenatal Injuries
 Heart Diseases Antibodies  >1 publications with Nefh and Heart Diseases
 Movement Disorders Antibodies  >1 publications with Nefh and Movement Disorders
 Memory Disorders Antibodies  >1 publications with Nefh and Memory Disorders
 Kidney Diseases Antibodies  >1 publications with Nefh and Kidney Diseases
 Fatty Liver Antibodies  >1 publications with Nefh and Fatty Liver
 Disease Models, Animal Antibodies  >1 publications with Nefh and Disease Models, Animal

Organs/Tissues associated with anti-Nefh antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >11 publications with Nefh and Brain
 Eye Antibodies  >2 publications with Nefh and Eye
 Spinal Cord Antibodies  >2 publications with Nefh and Spinal Cord
 Bone Marrow Antibodies  >2 publications with Nefh and Bone Marrow
 Inner Ear Antibodies  >1 publications with Nefh and Inner Ear
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