HIV-1 Reverse Transcriptase (HIV) Assay Kit

Background: Human Immunodeficiency Virus 1 (HIV 1) is a retrovirus that contains, as part of its genome, a reverse transcriptase (RT) gene, that when translated into protein, reverse transcribes HIVs RNA genome into a transducing DNA copy (cDNA). This HIV RT activity is critical during early viral infection. HIV 1 infection results in host cell domination, viral replication, and host cell death. HIV RT is an optimistic target for blocking the virus life cycle and the spread of the virus to neighboring cells. In addition, HIV RT in viral conditioned medium directly correlates with virus propagation in cell-mediated in vitro experimental model systems, therefore HIV RT activity can be used as a marker for HIV 1 growth. MBS, Inc. has developed a 2-hour HIV RT assay for drug screening and a 24-hour assay to detect as little as 1 pg of HIV RT isolated from viral conditioned media. An assay incubation time of 5-6 hours allows the experiment to be completed in one day and will detect 2 pg of HIV RT. The RT assay is qualitative, nonradioactive, colorimetric, and can be used to determine if test articles, compounds, or peptides can inhibit HIV RT activity. The assay can also be used to evaluate HIV RT activity in experimental samples.

Small volumes of HIV-1 Reverse Transcriptase assay kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS412605 is an assay kit targeting HIV-1 Reverse Transcriptase.