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ATG9B Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS224352

MBS224352 recognises human Autophagy-related protein 9B (ATG9B), a 924 amino acid multi-pass membrane protein belonging to the ATG9 family. More details

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Sample Manual Insert View PDF
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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Product Name
ATG9B, Polyclonal Antibody
Full Product Name
RABBIT ANTI ATG9B
Product Gene Name
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Mouse, Rat
Form/Format
Purified
Purified IgG - liquid
Concentration
Ig concentration 1.0mg/ml (lot specific)
Specificity
This item recognises human Autophagy-related protein 9B (ATG9B), a 924 amino acid multi-pass membrane protein belonging to the ATG9 family.
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted.
Shelf Life: 18 months from date of despatch.
Molecular Weight
36,989 Da
OMIM
612205
Perservative Stabilisers
0.02% Sodium Azide (NaN3)
Preparation||Antisera to Human ATG9B were raised by repeated immunisation of Rabbits with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography.
Immunogen
A synthetic peptide corresponding to 17 amino acid sequence from near the carboxy terminus of human ATG9B
Histology Positive Control Tissue||HeLa Cells
Buffer Solution||Phosphate buffered saline.
Target Species
Human
Applications Tested/Suitable for anti-ATG9B antibody
Immunohistology Frozen, ELISA (EIA), Western Blot (WB)
Application Notes for anti-ATG9B antibody
ELISA: This product is suitable for use in indirect ELISA applications.
Western Blot: This item detects a band of approximately 115 kDa in HeLa cell lysates.
Immunohistology - Frozen: Minimum Dilution: 10ug/ml
Western Blotting: Minimum Dilution: 1-2ug/ml

Testing Data #1 of anti-ATG9B antibody

Western blot analysis of HeLa cell lysate probed with Rabbit anti Human ATG9B (MBS224352) at 1.0(A) and 2.0(B) ugml-1
Rabbit Anti-ATG9B Polyclonal Antibody – Cat# MBS224352

Testing Data #2 of anti-ATG9B antibody

Immunocytochemical staining of HeLa cells with Rabbit anti Human ATG9B (MBS224352)
Rabbit Anti-ATG9B Polyclonal Antibody, Testing Data #2 – Cat# MBS224352

Other Notes

Small volumes of anti-ATG9B antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ATG9B purchase

MBS224352 is an antibody targeting ATG9B. Also known as RABBIT ANTI ATG9B. Reactive in Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for ATG9B. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
autophagy-related protein 9B
NCBI Official Synonym Full Names
autophagy related 9B
NCBI Official Symbol
ATG9B
NCBI Official Synonym Symbols
SONE; APG9L2; NOS3AS
UniProt Protein Name
Autophagy-related protein 9B
UniProt Synonym Protein Names
APG9-like 2; Nitric oxide synthase 3-overlapping antisense gene protein; Protein sONE
UniProt Gene Name
ATG9B
UniProt Synonym Gene Names
APG9L2; NOS3AS; Protein sONE
UniProt Entry Name
ATG9B_HUMAN
NCBI Protein Information
autophagy-related protein 9B; APG9-like 2; ATG9 autophagy related 9 homolog B; autophagy 9-like 2 protein; endothelial nitric oxide synthase antisense; nitric oxide synthase 3-overlapping antisense gene protein

NCBI Summary for ATG9B

This gene functions in the regulation of autophagy, a lysosomal degradation pathway. This gene also functions as an antisense transcript in the posttranscriptional regulation of the endothelial nitric oxide synthase 3 gene, which has 3' overlap with this gene on the opposite strand. Mutations in this gene and disruption of the autophagy process have been associated with multiple cancers. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2012]
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UniProt Comments for ATG9B

ATG9B: Plays a role in autophagy. Belongs to the ATG9 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Vesicle; Membrane protein, integral; Membrane protein, multi-pass; Autophagy

Chromosomal Location of Human Ortholog: 7q36.1

Cellular Component: integral to membrane; cytoplasmic vesicle

Biological Process: autophagic vacuole formation
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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