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Nf KAPPA B Inhibitor ALPHA (NFKBIA) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS224835

Mouse anti NF kappa B inhibitor alpha antibody, clone 6A055, recognizes NF kappa B inhibitor alpha also known as I-kappa-B-alpha (IkB-alpha) and major histocompatibility complex enhancer-binding protein MAD3. NF kappa B inhibitor alpha is one of seven members… More details

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Product Overview

Product Name
NF KAPPA B INHIBITOR ALPHA (NFKBIA), Monoclonal Antibody
Full Product Name
MOUSE ANTI NF KAPPA B INHIBITOR ALPHA
Product Gene Name
Clonality
Monoclonal
Isotype
IgG1
Clone Number
6A055
Host
Mouse
Species Reactivity
Human
Form/Format
Purified IgG - liquid
Concentration
IgG concentration 0.25 mg/ml (lot specific)
Specificity
NF KAPPA B INHIBITOR ALPHA
Preparation and Storage
Store at -20 degree C only. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 12 months from date of despatch
Molecular Weight
35,609 Da
OMIM
164008
3D Structure
ModBase 3D Structure for P25963
Preparation
Purified IgG prepared by affinity chromatography
Preservative Stabilisers
0.02% Thiomersal, 1% Bovine Serum Albumin, 50% Glycerol
Buffer Solution
Phosphate buffered saline
Applications Tested/Suitable for anti-NFKBIA antibody
Immunoprecipitation (IP), Western Blot (WB)
Application Notes for anti-NFKBIA antibody
Immunoprecipitation: 1.0; 4.0 ug/ml
Western Blot: 0.5; 4.0 ug/ml

Western Blot (WB) of anti-NFKBIA antibody

Western blot analysis of Jurkat cell lysate treated with (right lane) or without (left lane) TNF alpha (1.0 nM) and probed with Mouse anti NF kappa B inhibitor alpha. The antibody recognizes both the phosphorylated and non-phosphorylated forms of NF kappa B inhibitor alpha
Mouse Anti-NFKBIA Monoclonal Antibody – Cat# MBS224835

Other Notes

Small volumes of anti-NFKBIA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NFKBIA purchase

MBS224835 is an antibody targeting NFKBIA. Also known as MOUSE ANTI NF KAPPA B INHIBITOR ALPHA. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for NFKBIA. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
NF-kappa-B inhibitor alpha
NCBI Official Synonym Full Names
NFKB inhibitor alpha
NCBI Official Symbol
NFKBIA
NCBI Official Synonym Symbols
IKBA; MAD-3; NFKBI
UniProt Protein Name
NF-kappa-B inhibitor alpha
UniProt Synonym Protein Names
I-kappa-B-alpha; IkB-alpha; IkappaBalpha; Major histocompatibility complex enhancer-binding protein MAD3
Protein Family
UniProt Gene Name
NFKBIA
UniProt Synonym Gene Names
IKBA; MAD3; NFKBI; IkB-alpha; IkappaBalpha
UniProt Entry Name
IKBA_HUMAN
NCBI Protein Information
NF-kappa-B inhibitor alpha

NCBI Summary for NFKBIA

This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease. [provided by RefSeq, Aug 2011]
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UniProt Comments for NFKBIA

Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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