ANNEXIN V (ANXA5) Assay Kit

Name: ANXA5 Assay Kit; ANNEXIN V; MyBioSource
Brand: MyBioSource
SKU: MBS224959
Price: 855 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS224959

This test employs the property of Annexin V to bind to the membrane phospholipid phosphatidylserine (PS) in the presence of Ca2+. PS is exposed at the cell surface during the early stages of apoptosis. Detection of PS is a very sensitive method for detecting… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Product Name

ANNEXIN V (ANXA5), Assay Kit

Full Product Name

ANNEXIN V: PE ASSAY KIT

Product Gene Name

ANXA5 assay kit

Form/Format

RPE

Specificity

ANNEXIN V KIT

Preparation and Storage

Store at +4 degree C. DO NOT FREEZE. This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 12 months from date of despatch

Molecular Weight

35,937 Da

OMIM

131230

3D Structure

ModBase 3D Structure for P08758

Preservative Stabilisers

0.09% Sodium Azide (NaN3), 1% Bovine Serum Albumin

Instructions For Use

1) Dilute the10X Binding Buffer to 1X in distilled water (1 ml Binding Buffer + 9 ml distilled water).
2) Wash cells once in PBS by gentle shaking or pipetting up and down. Then wash once in 1X Binding Buffer
3) Resuspend cells in 1X Binding Buffer, adjusting to a cell density of 1-5 x 106 cells/ml
4) Add 5 ul of PE conjugated Annexin V to 100 ul of the cell suspension.
5) Mix and incubate for 10 to15 minutes at room temperature.
6) Wash cells in 1X Binding Buffer.
7) Resuspend cells in 200 ul of 1X Binding Buffer.
8) Add 5 ul of 7-AAD Viability Staining Solution and incubate for 5 minutes at room temperature.
9) Analyse by flow cytometry within 4 hours. Store at 2-8 degree C in the dark.

Applications Tested/Suitable for ANXA5 assay kit

Flow Cytometry (FC/FACS)

Other Notes

Small volumes of ANXA5 assay kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ANXA5 purchase

MBS224959 is an assay kit targeting ANXA5. Also known as ANNEXIN V: PE ASSAY KIT.

Additional Details

NCBI/Uniprot data below describe general gene information for ANXA5. It may not necessarily be applicable to this product.

NCBI GI #

113960

NCBI GeneID

308

NCBI Accession #

P08758.2

UniProt Primary Accession #

P08758

UniProt Secondary Accession #

Q6FHB3; Q6FI16; Q8WV69; Q9UDH9; D3DNW7

UniProt Related Accession #

P08758

NCBI Official Full Name

Annexin A5

NCBI Official Synonym Full Names

annexin A5

NCBI Official Symbol

ANXA5

NCBI Official Synonym Symbols

PP4; ANX5; ENX2; RPRGL3; HEL-S-7

UniProt Protein Name

Annexin A5

UniProt Synonym Protein Names

Anchorin CII; Annexin V; Annexin-5; Calphobindin I; CBP-I; Endonexin II; Lipocortin V; Placental anticoagulant protein 4; PP4; Placental anticoagulant protein I; PAP-I; Thromboplastin inhibitor; Vascular anticoagulant-alpha; VAC-alpha

Protein Family

Annexin

UniProt Gene Name

ANXA5

UniProt Synonym Gene Names

ANX5; ENX2; PP4; CBP-I; PP4; PAP-I; VAC-alpha

UniProt Entry Name

ANXA5_HUMAN

NCBI Protein Information

annexin A5

NCBI Summary for ANXA5

The protein encoded by this gene belongs to the annexin family of calcium-dependent phospholipid binding proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII. The gene spans 29 kb containing 13 exons, and encodes a single transcript of approximately 1.6 kb and a protein product with a molecular weight of about 35 kDa. [provided by RefSeq, Jul 2008]

UniProt Comments for ANXA5

This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.

Research Articles on ANXA5

1. Annexin V is fused to an enzyme, creating a fusion protein that converts nontoxic drug precursors, prodrugs, into anticancer compounds while bound to the tumor, therefore mitigating the risk of side effects.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with ANXA5 assay kit

Reference Product	PubMed Publications
CFTR assay kit	>3 publications with ANXA5 and CFTR
LGALS1 assay kit	>2 publications with ANXA5 and LGALS1
S100B assay kit	>2 publications with ANXA5 and S100B
SERPINH1 assay kit	>1 publications with ANXA5 and SERPINH1
ICAM1 assay kit	>1 publications with ANXA5 and ICAM1
PKD1 assay kit	>1 publications with ANXA5 and PKD1

Pathways associated with ANXA5 assay kit

Products by Pathway	Pathway Diagram
Prostaglandin Synthesis And Regulation Pathway antibodies	Prostaglandin Synthesis And Regulation Pathway Diagram

Diseases associated with ANXA5 assay kit

Disease Name	Pubmed Publications
Necrosis Antibodies	>556 publications with ANXA5 and Necrosis
Cardiovascular Diseases Antibodies	>410 publications with ANXA5 and Cardiovascular Diseases
Inflammation Antibodies	>265 publications with ANXA5 and Inflammation
Disease Models, Animal Antibodies	>159 publications with ANXA5 and Disease Models, Animal
Heart Diseases Antibodies	>149 publications with ANXA5 and Heart Diseases
Liver Diseases Antibodies	>104 publications with ANXA5 and Liver Diseases
Neoplasms, Experimental Antibodies	>98 publications with ANXA5 and Neoplasms, Experimental
Liver Neoplasms Antibodies	>77 publications with ANXA5 and Liver Neoplasms
Lung Neoplasms Antibodies	>75 publications with ANXA5 and Lung Neoplasms
Anemia Antibodies	>74 publications with ANXA5 and Anemia

Organs/Tissues associated with ANXA5 assay kit

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>1472 publications with ANXA5 and Blood
Vascular Antibodies	>346 publications with ANXA5 and Vascular
Brain Antibodies	>255 publications with ANXA5 and Brain
Muscle Antibodies	>220 publications with ANXA5 and Muscle
Heart Antibodies	>208 publications with ANXA5 and Heart
Liver Antibodies	>202 publications with ANXA5 and Liver
Lung Antibodies	>193 publications with ANXA5 and Lung
Bone Antibodies	>183 publications with ANXA5 and Bone
Kidney Antibodies	>140 publications with ANXA5 and Kidney
Eye Antibodies	>119 publications with ANXA5 and Eye