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ATG16L1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS225082

Rabbit anti ATG16L1 antibody recognizes autophagy-related protein 16-1 (ATG16L1), also known as APG16-LIKE 1. ATG16 is a key autophagy protein that forms a complex with ATG12-ATG5. This complex plays a critical role in the formation of the autophagosome. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
ATG16L1, Polyclonal Antibody
Full Product Name
Rabbit anti ATG16L1
Product Gene Name
Product Synonym Names
ATG16L1 Antibody
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse (Expected from Sequence), Rat (Expected from Sequence)
Form/Format
Liquid in phosphate buffered saline with 0.02% sodium azide, 50% glycerol.
Purity/Purification
Purified whole serum by affinity chromatography.
Preparation and Storage
Store at -20 degree C.
Storage in frost-free freezers is not recommended. This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 12 months from date of despatch
Molecular Weight
49,486 Da
OMIM
610767
3D Structure
ModBase 3D Structure for Q676U5
Immunogen
Recombinant human ATG16L1
Preperation
Antiserum to ATG16L1 was raised by repeated immunization of rabbits with highly purified antigen.
Applications Tested/Suitable for anti-ATG16L1 antibody
Immunofluorescence (IF), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes for anti-ATG16L1 antibody
IF: 1:50-1:200
IHC-Paraffin: 1:50-1:200
WB: 1:500-1:2000

Western Blot (WB) of anti-ATG16L1 antibody

Western blot analysis of cell lysates using Rabbit anti ATG16L1 antibody
Rabbit Anti-ATG16L1 Polyclonal Antibody – Cat# MBS225082

Immunohistochemistry (IHC) of anti-ATG16L1 antibody

Immunohistochemistry analysis of paraffin-embedded human colon cancer using Rabbit anti ATG16L1 antibody
Rabbit Anti-ATG16L1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS225082

Immunofluorescence (IF) of anti-ATG16L1 antibody

Immunofluorescence analysis of A549 cells using Rabbit anti ATG16L1 antibody. DAPI (Blue) was used for nuclear staining
Rabbit Anti-ATG16L1 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS225082

Other Notes

Small volumes of anti-ATG16L1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ATG16L1 purchase

MBS225082 is an antibody targeting ATG16L1. Also known as Rabbit anti ATG16L1. Reactive in Human, Mouse (Expected from Sequence), Rat (Expected from Sequence). Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for ATG16L1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
Autophagy-related protein 16-1
NCBI Official Synonym Full Names
autophagy related 16 like 1
NCBI Official Symbol
ATG16L1
NCBI Official Synonym Symbols
IBD10; WDR30; APG16L; ATG16A; ATG16L
UniProt Protein Name
Autophagy-related protein 16-1
UniProt Synonym Protein Names
APG16-like 1
UniProt Gene Name
ATG16L1
UniProt Synonym Gene Names
APG16L
NCBI Protein Information
autophagy-related protein 16-1

NCBI Summary for ATG16L1

The protein encoded by this gene is part of a large protein complex that is necessary for autophagy, the major process by which intracellular components are targeted to lysosomes for degradation. Defects in this gene are a cause of susceptibility to inflammatory bowel disease type 10 (IBD10). Several transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jun 2010]
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UniProt Comments for ATG16L1

Plays an essential role in autophagy: interacts with ATG12-ATG5 to mediate the conjugation of phosphatidylethanolamine (PE) to LC3 (MAP1LC3A, MAP1LC3B or MAP1LC3C), to produce a membrane-bound activated form of LC3 named LC3-II. Thereby, controls the elongation of the nascent autophagosomal membrane (PubMed:24553140, PubMed:23376921, PubMed:24954904, PubMed:27273576, PubMed:23392225). Regulates mitochondrial antiviral signaling (MAVS)-dependent type I interferon (IFN-I) production (PubMed:25645662). Negatively regulates NOD1- and NOD2-driven inflammatory cytokine response (PubMed:24238340). Instead, promotes with NOD2 an autophagy-dependent antibacterial pathway (PubMed:20637199). Plays a role in regulating morphology and function of Paneth cell (PubMed:18849966).
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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