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14-3-3 Epsilon (YWHAE) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS225502

MBS225502 is an antibody targeting YWHAE. Also known as Mouse anti 14-3-3 Epsilon. Reactive in Human, Mouse, Rat. Host: Mouse. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
14-3-3 Epsilon (YWHAE), Monoclonal Antibody
Full Product Name
Mouse anti 14-3-3 Epsilon
Product Gene Name
Product Synonym Names
14-3-3 Epsilon Antibody
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
AB01/1G9
Host
Mouse
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid in phosphate buffered saline with 0.09% sodium azide (NaN3).
Purity/Purification
Purified by affinity chromatography on Protein G from tissue culture supernatant
Preparation and Storage
Store undiluted at -20 degree C. Avoid repeated freeze/thaw cycles. Shelf Life: As supplied, 12 months from date of despatch.
Molecular Weight
28 kDa
OMIM
605066
3D Structure
ModBase 3D Structure for P62258
Immunogen
E Coli-derived recombinant human 14-3-3 epsilon (amino acids 1 - 255)
Applications Tested/Suitable for anti-YWHAE antibody
Western Blot (WB)
Application Notes for anti-YWHAE antibody
WB: 1:1000
Western Blotting: Detects a band of approximately 28 kDa in HEK293 cell lysates.

Western Blot (WB) of anti-YWHAE antibody

Western blot analysis of whole cell lysates probed with 14-3-3 epsilon antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000) and visualized on the ChemiDoc MP with 67 second exposure. Arrow points to 14-3-3 epsilon (molecular weight 28 kDa)
Mouse Anti-YWHAE Monoclonal Antibody – Cat# MBS225502

Other Notes

Small volumes of anti-YWHAE antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for YWHAE. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
14-3-3 protein epsilon
NCBI Official Synonym Full Names
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein epsilon
NCBI Official Symbol
YWHAE
NCBI Official Synonym Symbols
MDS; HEL2; MDCR; KCIP-1; 14-3-3E
UniProt Protein Name
14-3-3 protein epsilon
Protein Family
UniProt Gene Name
YWHAE
UniProt Synonym Gene Names
14-3-3E
NCBI Protein Information
14-3-3 protein epsilon

NCBI Summary for YWHAE

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. It interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. Two transcript variants, one protein-coding and the other non-protein-coding, have been found for this gene. [provided by RefSeq, Aug 2008]
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UniProt Comments for YWHAE

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner (). Positively regulates phosphorylated protein HSF1 nuclear export to the cytoplasm (PubMed:12917326).
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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