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EBAG9 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS225526

MBS225526 is an antibody targeting EBAG9. Also known as EBAG9 Antibody with Control Lysate. Reactive in Human, Mouse. Host: Mouse. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
EBAG9, Monoclonal Antibody
Full Product Name
EBAG9 Antibody with Control Lysate
Product Gene Name
Product Synonym Names
Mouse anti EBAG9; EBAG9 Antibody
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
CPTC14
Host
Mouse
Species Reactivity
Human, Mouse
Form/Format
Liquid in phosphate buffered saline with 0.09% sodium azide (NaN3).
Purity/Purification
Purified by affinity chromatography on Protein G from tissue culture supernatant
Preparation and Storage
Antibody: Store undiluted at -20 degree C, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20 degree C.
After reconstitution aliquot and store at -20 degree C for up to 3 months or at -80 degree C for longer term storage. Shelf Life: As supplied, 12 months from date of despatch.
Molecular Weight
30 kDa
OMIM
605772
3D Structure
ModBase 3D Structure for O00559
Immunogen
Recombinant protein of human EBAG9
Lysate Composition
400ug K562 lysate lyophilized in RIPA buffer
Lysate Reconstitution
If using DDT reconstitute the lyophilized lysate with 190mul DI H2O, add 200mul 2x Laemmli Sample Buffer and 10mul 2M DTT.
If using BME reconstitute the lyophilized lysate with 180mul DI H2O, add 200mul 2x Laemmli Sample Buffer and 20u
Applications Tested/Suitable for anti-EBAG9 antibody
Western Blot (WB)
Application Notes for anti-EBAG9 antibody
WB: 1:1000
Western Blotting: Detects a band of approximately 30 kDa in K562 cell lysates.

Western Blot (WB) of anti-EBAG9 antibody

Western blot analysis of whole cell lysates probed with EBAG9 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000) and visualized on the ChemiDoc MP with 10 second exposure. Arrow points to EBAG9 (molecular weight 30 kDa)
Mouse Anti-EBAG9 Monoclonal Antibody – Cat# MBS225526

Other Notes

Small volumes of anti-EBAG9 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for EBAG9. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
Receptor-binding cancer antigen expressed on SiSo cells
NCBI Official Synonym Full Names
estrogen receptor binding site associated, antigen, 9
NCBI Official Symbol
EBAG9
NCBI Official Synonym Symbols
EB9; PDAF
UniProt Protein Name
Receptor-binding cancer antigen expressed on SiSo cells
UniProt Synonym Protein Names
Cancer-associated surface antigen RCAS1; Estrogen receptor-binding fragment-associated gene 9 protein
UniProt Gene Name
EBAG9
UniProt Synonym Gene Names
RCAS1
NCBI Protein Information
receptor-binding cancer antigen expressed on SiSo cells

NCBI Summary for EBAG9

This gene was identified as an estrogen-responsive gene. Regulation of transcription by estrogen is mediated by estrogen receptor, which binds to the estrogen-responsive element found in the 5'-flanking region of this gene. The encoded protein is a tumor-associated antigen that is expressed at high frequency in a variety of cancers. Alternate splicing results in multiple transcript variants. A pseudogene of this gene has been defined on chromosome 10. [provided by RefSeq, Jul 2013]
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UniProt Comments for EBAG9

May participate in suppression of cell proliferation and induces apoptotic cell death through activation of interleukin-1-beta converting enzyme (ICE)-like proteases.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-EBAG9 antibody

 Reference Product  PubMed Publications
 SNAPIN antibody  >2 publications with EBAG9 and SNAPIN
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