Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse Ly-6C antibody, clone ER-MP20 followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody . Low power
Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse Ly-6C antibody, clone ER-MP20 followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody . Medium power
Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse Ly-6C antibody, clone ER-MP20 followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody . High power
Rat anti Mouse Ly6C antibody, clone ER-MP20 used for the evaluation of Ly-6C expression on inflammatory macrophages in a brain injury model by flow cytometry.
Image caption:
Dual targeting of CCR2/5 with CVC mitigates TBI-induced macrophage recruitment. a In WT mice, CVC or vehicle was administered BID via oral gavage at 100 mg/kg at 2 and 10 h post surgery before animals were euthanized for various endpoints at 24 h following surgery. b A cohort of WT aged TBI animals (n?=?8/group) was used for flow cytometry analysis of macrophage infiltration into the injured brain. Twenty-four hours after injury, there was a significant decrease in the number of peripheral macrophages (CD11b+F4/80+CD45hiLy6C+) in the CVC-treated animals compared to their vehicle-treated counterparts
From: Morganti JM, Riparip LK, Chou A, Liu S, Gupta N, Rosi S.
Age exacerbates the CCR2/5-mediated neuroinflammatory response to traumatic brain injury.
J Neuroinflammation. 2016 Apr 18;13(1):80.
This is from an open access article distributed under the terms of the Creative Commons Attribution License.
Figure A. RPE conjugated Rat anti Mouse CD8a and A700 conjugated Rat IgG2a isotype control . Figure B. RPE conjugated Rat anti Mouse CD8a and A700 conjugated Rat anti Mouse Ly6C . All experiments performed on red cell lysed mouse blood gated on mononuclear cells in the presence of Mouse Seroblock (BUF041A). Data acquired on the ZE5 cell analyzer.
Figure A. RPE conjugated Rat anti Mouse CD8a and Pacific Blue conjugated Rat IgG2a isotype control . Figure B. RPE conjugated Rat anti Mouse CD8a and Pacific Blue conjugated Rat anti Mouse Ly6C . All experiments performed on red cell lysed mouse blood gated on mononuclear cells in the presence of Mouse Seroblock (BUF041A). Data acquired on the ZE5 cell analyzer.
Figure A. FITC conjugated Rat anti Mouse CD8 and RPE conjugated Rat IgG2a isotype control . Figure B. FITC conjugated Rat anti Mouse CD8 and RPE conjugated Rat anti Mouse Ly-6C . All experiments performed on mouse splenocytes in the presence of murine SeroBlock (BUF041A).
Figure A. RPE conjugated Rat anti Mouse CD8a and FITC conjugated Rat IgG2a isotype control . Figure B. RPE conjugated Rat anti Mouse CD8a and FITC conjugated Rat anti Mouse Ly6C . All experiments performed on red cell lysed mouse blood gated on lymphocytes in the presence of 10% mouse serum. Data acquired on the ZE5 Cell Analyzer.
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