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CD52 Antibody – Human Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS225702

Description: Anti-CD52 Antibody is a non-therapeutic biosimilar of the monoclonal antibody drug alemtuzumab (Lemtrada) for research use. It can be used in bioanalytical assays and for studying biological pathways affected by the drug.Summary: Human Anti-CD52… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
CD52, Monoclonal Antibody
Full Product Name
Human Anti CD52 (Alemtuzumab Biosimilar)
Product Gene Name
anti-CD52 antibody
Product Synonym Names
CD52 antibody (Campath-1H)
Clonality
Monoclonal
Isotype
IgG1
Clone Number
Campath-1H
Host
Human
Species Reactivity
Human
Form/Format
Liquid; Phosphate Buffered Saline
Purity/Purification
>98% by SDS PAGE
Concentration
1.0 mg/ml (lot specific)
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
3D Structure
ModBase 3D Structure for P31358
Immunogen
Human lymphocytes
Preservative Stabilizers
0.02% Proclin 300
Applications Tested/Suitable for anti-CD52 antibody
ELISA (EIA)

Testing Data of anti-CD52 antibody

Alemtuzumab bridging ELISA for pharmacokinetic (PK) assay development
A microtiter plate was coated overnight with Human Anti-Alemtuzumab Antibody (clone AbD19189, HCA198) at a concentration of 1 mug/ml. After washing and blocking with PBST + 5% BSA, 10% human serum was added spiked with increasing concentrations of alemtuzumab (therapeutic reference product) or Human Anti-CD52 Antibody, MCA6101 (alemtuzumab biosimilar, research grade). Detection was performed using HRP conjugated Human Anti-Alemtuzumab Antibody (clone AbD16942_hIgG1, HCA175P) at a concentration of 2 mug/ml in HISPEC Assay Diluent (BUF049A) and QuantaBlu Fluorogenic Peroxidase Substrate. Data are shown as the mean of three measurements.
Human Anti-CD52 Monoclonal Antibody – Cat# MBS225702

Testing Data of anti-CD52 antibody

Purity assessment by SDS-PAGE
0.5 mug of Human Anti-CD52 Antibody, MCA6101 (alemtuzumab biosimilar, research grade) and alemtuzumab (therapeutic reference product) were denatured and analyzed under reducing (R) and non-reducing (NR) conditions. Bands were visualized using Coomassie staining. Full IgG (H2L2), heavy (H) and light chains (L) are indicated.
Human Anti-CD52 Monoclonal Antibody, Testing Data – Cat# MBS225702

Testing Data of anti-CD52 antibody

Monodispersity assessment by HPLC-SEC
Size exclusion chromatography (SEC) of Human Anti-CD52 Antibody, MCA6101 (alemtuzumab biosimilar, research grade) and alemtuzumab (therapeutic reference product) was performed on an analytical HPLC instrument. Shown are monomer peaks (H2L2), calculated relative monomer portion and aggregates. Column: MAbPac SEC-1, 5 mum; dimension: 4.0 x 150 mm; mobile phase: 50 mM sodium phosphate, pH 6.8, 300 mM NaCl, 200 mM Arginine; flow rate: 200 mul/min; inj. volume [mul]: 3; temp.: 25 degree C; detection: 280 nm; sample: mAb (1 mg/ml).
Human Anti-CD52 Monoclonal Antibody, Testing Data – Cat# MBS225702

Other Notes

Small volumes of anti-CD52 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD52 purchase

MBS225702 is an antibody targeting CD52. Also known as Human Anti CD52 (Alemtuzumab Biosimilar). Reactive in Human. Host: Human.

Additional Details

NCBI/Uniprot data below describe general gene information for CD52. It may not necessarily be applicable to this product.

NCBI GI #
399214
NCBI GeneID
1043
NCBI Accession #
P31358.1
UniProt Primary Accession #
P31358
UniProt Related Accession #
P31358
NCBI Official Full Name
CAMPATH-1 antigen
NCBI Official Synonym Full Names
CD52 molecule
NCBI Official Symbol
CD52
NCBI Official Synonym Symbols
HE5; CDW52; EDDM5
UniProt Protein Name
CAMPATH-1 antigen
UniProt Synonym Protein Names
CDw52; Cambridge pathology 1 antigen; Epididymal secretory protein E5; Human epididymis-specific protein 5; He5; CD_antigen: CD52
Protein Family
CAMPATH-1 antigen
UniProt Gene Name
CD52
UniProt Synonym Gene Names
CDW52; HE5; He5
NCBI Protein Information
CAMPATH-1 antigen

UniProt Comments for CD52

May play a role in carrying and orienting carbohydrate, as well as having a more specific role.

Research Articles on CD52

1. This polyclonal mutational landscape in the PIGA gene was also found in CD52(-) T cells present.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CD52 antibody

 Reference Product  PubMed Publications
 CD4 antibody  >103 publications with CD52 and CD4
 CD19 antibody  >55 publications with CD52 and CD19
 CD22 antibody  >38 publications with CD52 and CD22
 CD5 antibody  >27 publications with CD52 and CD5
 CD7 antibody  >8 publications with CD52 and CD7
 NPC2 antibody  >5 publications with CD52 and NPC2
 CRISP1 antibody  >2 publications with CD52 and CRISP1
 GPR64 antibody  >2 publications with CD52 and GPR64

Pathways associated with anti-CD52 antibody

 Products by Pathway  Pathway Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram
 Post-translational Modification: Synthesis Of GPI-anchored Proteins Pathway antibodies  Post-translational Modification: Synthesis Of GPI-anchored Proteins Pathway Diagram
 Post-translational Protein Modification Pathway antibodies  Post-translational Protein Modification Pathway Diagram

Diseases associated with anti-CD52 antibody

 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >44 publications with CD52 and Cardiovascular Diseases
 Thrombocytopenia Antibodies  >37 publications with CD52 and Thrombocytopenia
 Inflammation Antibodies  >34 publications with CD52 and Inflammation
 Necrosis Antibodies  >33 publications with CD52 and Necrosis
 Infertility, Male Antibodies  >19 publications with CD52 and Infertility, Male
 Lung Diseases Antibodies  >18 publications with CD52 and Lung Diseases
 Kidney Diseases Antibodies  >16 publications with CD52 and Kidney Diseases
 Disease Models, Animal Antibodies  >13 publications with CD52 and Disease Models, Animal
 Liver Diseases Antibodies  >12 publications with CD52 and Liver Diseases
 Neoplasms, Experimental Antibodies  >5 publications with CD52 and Neoplasms, Experimental

Organs/Tissues associated with anti-CD52 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >415 publications with CD52 and Blood
 Bone Marrow Antibodies  >146 publications with CD52 and Bone Marrow
 Kidney Antibodies  >67 publications with CD52 and Kidney
 Epididymis Antibodies  >37 publications with CD52 and Epididymis
 Lymph Node Antibodies  >24 publications with CD52 and Lymph Node
 Lymph Antibodies  >24 publications with CD52 and Lymph
 Lung Antibodies  >21 publications with CD52 and Lung
 Spleen Antibodies  >19 publications with CD52 and Spleen
 Heart Antibodies  >17 publications with CD52 and Heart
 Brain Antibodies  >17 publications with CD52 and Brain
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