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CD11b Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS225942

Mouse anti Bovine CD11b antibody, clone CC126 recognizes the bovine CD11b cell surface antigen. More details

$885 / 100 Tests +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD11b, Monoclonal Antibody
Full Product Name
MOUSE ANTI BOVINE CD11b: RPE
Product Gene Name
Product Synonym Names
CD11b antibody|CC126
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
CC126
Host
Mouse
Species Reactivity
Bovine
Form/Format
Purified IgG conjugated to R. Phycoerythrin (RPE), lyophilized. Phosphate buffered saline
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Specificity
CD11b
Preparation and Storage
Store at 4 degree C. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Species Cross-Reactivity
Goat, Sheep
Preservative Stabilizers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line
Reconstitution
Reconstitute with 1.0 ml distilled water
Applications Tested/Suitable for anti-CD11b antibody
Flow Cytometry (FC/FACS)
Application Notes for anti-CD11b antibody
FC/FACS: Max Dilution: Neat
FC/FACS: Use 10ul of the suggested working dilution to label 106 cells in 100ul

Testing Data of anti-CD11b antibody

Figure A. RPE conjugated Mouse anti Bovine CD335 and FITC conjugated Mouse IgG2b isotype control . Figure B. RPE conjugated Mouse anti Bovine CD335 and FITC conjugated Mouse anti Bovine CD11b . All experiments performed on red cell lysed bovine blood gated on live cells in the presence of 10% bovine serum. Data acquired on the ZE5 Cell Analyzer.
Mouse Anti-CD11b Monoclonal Antibody – Cat# MBS225942

Testing Data of anti-CD11b antibody

Figure A. Purified mouse anti bovine CD13 detected with goat anti mouse IgG1 RPE and FITC conjugated mouse IgG2b isotype control . Figure B. Purified mouse anti bovine CD13 detected with goat anti mouse IgG1 RPE and FITC conjugated mouse anti bovine CD11b . All experiments performed on red cell lysed bovine blood gated on mononuclear cells.
Mouse Anti-CD11b Monoclonal Antibody, Testing Data – Cat# MBS225942

Testing Data of anti-CD11b antibody

Figure A. FITC conjugated mouse anti bovine CD11b and purified mouse IgG1 isotype control detected with RPE conjugated goat anti mouse IgG1 . Figure B. FITC conjugated mouse anti bovine CD11b and purified CD13 detected with RPE conjugated goat anti mouse IgG1 . All experiments performed on red cell lysed bovine blood gated on mononuclear cells.
Mouse Anti-CD11b Monoclonal Antibody, Testing Data – Cat# MBS225942

Testing Data of anti-CD11b antibody

Published customer image:
Mouse anti Bovine CD11b antibody, clone CC126 used as a blocking antibody preventing Emeria bovis infection of bovine neutrophils.
Image caption:
CD11b surface expression and CD11b-dependent-NET formation of bovine E. bovis sporozoite-exposed PMN. (A) Bovine PMN were exposed to CFSE-labeled sporozoites (1:1) and subjected to FACS analysis probing with an antibody directed against CD11b. Non-exposed PMN in plain medium served as negative controls. The histograms represent the shift in the mean fluorescence of the PMN population analyzed and are representative of the experiments performed. (B) NET formation in the presence of anti-CD11b antibodies. Bovine PMN were pre-treated with anti-CD11b prior to sporozoite exposure. Stimulation with zymosan served as a positive control; PMN in plain medium were used for negative controls.

From: Muñoz-Caro et al.
Eimeria bovis-triggered neutrophil extracellular trap formation is CD11b-, ERK 1/2-, p38 MAP kinase- and SOCE-dependent.
Veterinary Research 2015 46:23.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse Anti-CD11b Monoclonal Antibody, Testing Data – Cat# MBS225942

Testing Data of anti-CD11b antibody

Published customer image:
Mouse anti Bovine CD11b antibody, clone CC126 used for the detection of CD11b on ovine leukocytes by flow cytometry
Image caption:
Analysis of the phenotype of ovine NKp46+ populations. (A)Two-colour flow cytometry analysis of ovine PBMC co-stained with EC1.1 and a selection of monoclonal antibodies against CD2, CD4, CD8, CD11b, CD16, WC1, IgM and MHCII. WC1 and IgM are surface molecules expressed by subsets of gammadelta T-cells and B-cells respectively. animals.

From: Connelley T, Storset AK, Pemberton A, MacHugh N, Brown J, Lund H, Morrison IW.
NKp46 defines ovine cells that have characteristics corresponding to NK cells.
Vet Res. 2011 Feb 23;42:37.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse Anti-CD11b Monoclonal Antibody, Testing Data – Cat# MBS225942

Product References and Citations for anti-CD11b antibody

1. Howard, C.J. & Naessens, J. (1993) Summary of workshop findings for cattle (tables 1 and 2). Vet Immunol Immunopathol. 39 (1-3): 25-47.
2. Naessens, J. et al. (1993) Cross-reactivity of workshop antibodies with cells from domestic and wild ruminants. Vet Immunol Immunopathol. 39 (1-3): 283-90.
3. Akesson, C.P. et al. (2008) Phenotypic characterisation of intestinal dendritic cells in sheep. Dev Comp Immunol. 32: 837-49.
4. Lecchi, C. et al. (2008) Bovine alpha-1 acid glycoprotein can reduce the chemotaxis of bovine monocytes and modulate CD18 expression. Vet Res. 39: 50.
5. Connelley, T. et al. (2011) NKp46 defines ovine cells that have characteristics corresponding to NK cells. Vet Res. 42: 37.

Other Notes

Small volumes of anti-CD11b antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD11b purchase

MBS225942 is an antibody targeting CD11b. Also known as MOUSE ANTI BOVINE CD11b: RPE. Reactive in Bovine. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CD11b. It may not necessarily be applicable to this product.

Protein Family

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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