Figure A. RPE conjugated Mouse anti Bovine CD335 and FITC conjugated Mouse IgG2b isotype control . Figure B. RPE conjugated Mouse anti Bovine CD335 and FITC conjugated Mouse anti Bovine CD11b . All experiments performed on red cell lysed bovine blood gated on live cells in the presence of 10% bovine serum. Data acquired on the ZE5 Cell Analyzer.
Figure A. Purified mouse anti bovine CD13 detected with goat anti mouse IgG1 RPE and FITC conjugated mouse IgG2b isotype control . Figure B. Purified mouse anti bovine CD13 detected with goat anti mouse IgG1 RPE and FITC conjugated mouse anti bovine CD11b . All experiments performed on red cell lysed bovine blood gated on mononuclear cells.
Figure A. FITC conjugated mouse anti bovine CD11b and purified mouse IgG1 isotype control detected with RPE conjugated goat anti mouse IgG1 . Figure B. FITC conjugated mouse anti bovine CD11b and purified CD13 detected with RPE conjugated goat anti mouse IgG1 . All experiments performed on red cell lysed bovine blood gated on mononuclear cells.
Published customer image:
Mouse anti Bovine CD11b antibody, clone CC126 used as a blocking antibody preventing Emeria bovis infection of bovine neutrophils.
Image caption:
CD11b surface expression and CD11b-dependent-NET formation of bovine E. bovis sporozoite-exposed PMN. (A) Bovine PMN were exposed to CFSE-labeled sporozoites (1:1) and subjected to FACS analysis probing with an antibody directed against CD11b. Non-exposed PMN in plain medium served as negative controls. The histograms represent the shift in the mean fluorescence of the PMN population analyzed and are representative of the experiments performed. (B) NET formation in the presence of anti-CD11b antibodies. Bovine PMN were pre-treated with anti-CD11b prior to sporozoite exposure. Stimulation with zymosan served as a positive control; PMN in plain medium were used for negative controls.
From: Muñoz-Caro et al.
Eimeria bovis-triggered neutrophil extracellular trap formation is CD11b-, ERK 1/2-, p38 MAP kinase- and SOCE-dependent.
Veterinary Research 2015 46:23.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Published customer image:
Mouse anti Bovine CD11b antibody, clone CC126 used for the detection of CD11b on ovine leukocytes by flow cytometry
Image caption:
Analysis of the phenotype of ovine NKp46+ populations. (A)Two-colour flow cytometry analysis of ovine PBMC co-stained with EC1.1 and a selection of monoclonal antibodies against CD2, CD4, CD8, CD11b, CD16, WC1, IgM and MHCII. WC1 and IgM are surface molecules expressed by subsets of gammadelta T-cells and B-cells respectively. animals.
From: Connelley T, Storset AK, Pemberton A, MacHugh N, Brown J, Lund H, Morrison IW.
NKp46 defines ovine cells that have characteristics corresponding to NK cells.
Vet Res. 2011 Feb 23;42:37.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.