Mouse Anti-IgE Monoclonal Antibody – Cat# MBS226020

Purified Horse IgE detected in indirect ELISA with Mouse anti Horse IgE followed by Rabbit F(ab')2 anti Mouse IgG:HRP

IgE Antibody – Mouse Monoclonal

Name: IgE Antibody; Monoclonal; Mouse; Horse-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS226020
Price: 435 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS226020

Mouse anti Horse IgE, clone 3H10, recognizes native equine IgE and does not cross react with equine IgM, IgA or IgG.IgE is an immunoglobulin primarily produced from plasma cells and, in normal serum, present at very low concentrations.IgE is important in both… More details

$435 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

IgE, Monoclonal Antibody

Full Product Name

MOUSE ANTI HORSE IgE

Product Gene Name

anti-IgE antibody

Product Synonym Names

IgE antibody|3H10

Clonality

Monoclonal

Isotype

IgG1

Clone Number

3H10

Host

Mouse

Species Reactivity

Horse

Form/Format

Purified IgG, liquid. Phosphate buffered saline
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant

Concentration

IgG concentration 1.0mg/ml (lot specific)

Specificity

IgE

Preparation and Storage

Store at 4 degree C or at -20 degree C if preferred.
Storage in frost-free freezers is not recommended.This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

Immunogen

Equine IgE

Preservative Stabilizers

0.09% Sodium Azide (NaN3)

Fusion Partners

Spleen cells from immunised Balb/c mice were fused with cells of the P3X myeloma cell line

Carrier Free

Yes

Applications Tested/Suitable for anti-IgE antibody

Immunohistochemistry (IHC)-Frozen, Western Blot (WB), ELISA (EIA)

Application Notes for anti-IgE antibody

ELISA: 1/1000-1/10,000
ELISA: This product is suitable for use in indirect ELISA applications.
Flow Cytometry: Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting: Western blot analysis against affinity purified equine IgE using Mouse anti Horse IgE clone 3H10 demonstrates a band of approximately 80 kDa under reducing conditions and a band of approximately 200 kDa under non-reducing conditions which correspond with the expected molecular weight of equine Ig epsilon chain and the complete equine IgE molecule, respectively (Wilson, D.A. et al. 2006).

Product References and Citations for anti-IgE antibody

1. Wilson, D.A. et al. (2006) Production of monoclonal antibodies specific for native equine IgE and their application to monitor total serum IgE responses in Icelandic and non-Icelandic horses with insect bite dermal hypersensitivity.Vet Immunol Immunopathol.112: 56-70.

Other Notes

Small volumes of anti-IgE antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for IgE purchase

MBS226020 is an antibody targeting IgE. Also known as MOUSE ANTI HORSE IgE. Reactive in Horse. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for IgE. It may not necessarily be applicable to this product.

Protein Family

IgE-binding protein

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.