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DAO Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS9127298
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This gene encodes the peroxisomal enzyme D-amino acid oxidase. The enzyme is a flavoprotein which uses flavin adenine dinucleotide (FAD) as its prosthetic group. Its substrates include a wide variety of D-amino acids, but it is inactive on the naturally… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
DAO, Polyclonal Antibody
Full Product Name
DAO Polyclonal Antibody
Product Gene Name
anti-DAO antibody
Product Synonym Names
DAAO; OXDA; DAMOX
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Purity/Purification
Affinity Purification
Concentration
1mg/ml (lot specific)
Preparation and Storage
Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Molecular Weight
347
OMIM
124050
3D Structure
ModBase 3D Structure for P14920
Species
Human
Immunogen
Recombinant protein of human DAO
Calculated Molecular Weight
39kDa

Product Categories/Family for anti-DAO antibody

Polyclonal
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-DAO antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-DAO antibody
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200

Western Blot (WB) of anti-DAO antibody

Western blot analysis of extracts of mouse kidney, using DAO antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.
Rabbit Anti-DAO Polyclonal Antibody – Cat# MBS9127298

Immunofluorescence (IF) of anti-DAO antibody

Immunofluorescence analysis of MCF-7 cells using DAO antibody. Blue: DAPI for nuclear staining.
Rabbit Anti-DAO Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS9127298

Other Notes

Small volumes of anti-DAO antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for DAO purchase

MBS9127298 is an antibody targeting DAO. Also known as DAO Polyclonal Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for DAO. It may not necessarily be applicable to this product.

NCBI GI #
25453448
NCBI GeneID
1610
NCBI Accession #
P14920.3
UniProt Primary Accession #
P14920
UniProt Secondary Accession #
Q16758; Q8N6R2; B2R7I5
UniProt Related Accession #
P14920
NCBI Official Full Name
D-amino-acid oxidase
NCBI Official Synonym Full Names
D-amino-acid oxidase
NCBI Official Symbol
DAO
NCBI Official Synonym Symbols
DAAO; OXDA; DAMOX
UniProt Protein Name
D-amino-acid oxidase
Protein Family
2-oxoglutarate-dependent dioxygenase
UniProt Gene Name
DAO
UniProt Synonym Gene Names
DAMOX; DAAO; DAMOX; DAO
UniProt Entry Name
OXDA_HUMAN
NCBI Protein Information
D-amino-acid oxidase; D-amino acid oxidase

UniProt Comments for DAO

DAO: Regulates the level of the neuromodulator D-serine in the brain. Has high activity towards D-DOPA and contributes to dopamine synthesis. Could act as a detoxifying agent which removes D-amino acids accumulated during aging. Acts on a variety of D- amino acids with a preference for those having small hydrophobic side chains followed by those bearing polar, aromatic, and basic groups. Does not act on acidic amino acids. Belongs to the DAMOX/DASOX family.

Protein type: Amino Acid Metabolism - glycine, serine and threonine; Other Amino Acids Metabolism - D-Arginine and D-ornithine; EC 1.4.3.3; Amino Acid Metabolism - arginine and proline; Oxidoreductase

Chromosomal Location of Human Ortholog: 12q24

Cellular Component: peroxisomal membrane; peroxisomal matrix; mitochondrial outer membrane; peroxisome; cytosol

Molecular Function: protein dimerization activity; protein binding; cofactor binding; D-amino-acid oxidase activity; receptor binding

Biological Process: proline catabolic process; glyoxylate metabolic process; dopamine biosynthetic process; leucine metabolic process

Disease: Schizophrenia
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Research Articles on DAO

1. In the brain, the concentration of D-serine stored in cells is defined by the activity of D-amino acid oxidase.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-DAO antibody

 Reference Product  PubMed Publications
 SHMT1 antibody  >1 publications with DAO and SHMT1

Pathways associated with anti-DAO antibody

 Products by Pathway  Pathway Diagram
 Arginine And Proline Metabolism Pathway antibodies  Arginine And Proline Metabolism Pathway Diagram
 Arginine And Proline Metabolism Pathway antibodies  Arginine And Proline Metabolism Pathway Diagram
 D-Arginine And D-ornithine Metabolism Pathway antibodies  D-Arginine And D-ornithine Metabolism Pathway Diagram
 D-Arginine And D-ornithine Metabolism Pathway antibodies  D-Arginine And D-ornithine Metabolism Pathway Diagram
 Glycine, Serine And Threonine Metabolism Pathway antibodies  Glycine, Serine And Threonine Metabolism Pathway Diagram
 Glycine, Serine And Threonine Metabolism Pathway antibodies  Glycine, Serine And Threonine Metabolism Pathway Diagram
 Glyoxylate Metabolism Pathway antibodies  Glyoxylate Metabolism Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Amino Acids And Derivatives Pathway antibodies  Metabolism Of Amino Acids And Derivatives Pathway Diagram

Diseases associated with anti-DAO antibody

 Disease Name  Pubmed Publications
 Immune System Diseases Antibodies  >182 publications with DAO and Immune System Diseases
 Nervous System Diseases Antibodies  >150 publications with DAO and Nervous System Diseases
 Disease Models, Animal Antibodies  >115 publications with DAO and Disease Models, Animal
 Liver Diseases Antibodies  >111 publications with DAO and Liver Diseases
 Heart Diseases Antibodies  >93 publications with DAO and Heart Diseases
 Inflammation Antibodies  >93 publications with DAO and Inflammation
 Necrosis Antibodies  >81 publications with DAO and Necrosis
 Schizophrenia Antibodies  >73 publications with DAO and Schizophrenia
 Hemorrhage Antibodies  >47 publications with DAO and Hemorrhage
 Hypertension Antibodies  >44 publications with DAO and Hypertension

Organs/Tissues associated with anti-DAO antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >250 publications with DAO and Brain
 Liver Antibodies  >223 publications with DAO and Liver
 Kidney Antibodies  >146 publications with DAO and Kidney
 Muscle Antibodies  >96 publications with DAO and Muscle
 Spinal Cord Antibodies  >27 publications with DAO and Spinal Cord
 Eye Antibodies  >27 publications with DAO and Eye
 Testis Antibodies  >11 publications with DAO and Testis
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