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Donkey Anti-Rat IgG (H+L) Secondary Antibody – Donkey Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS674959

MBS674959 is a secondary antibody targeting Donkey Anti-Rat IgG (H+L). Also known as Donkey Anti-Rat IgG (H+L), Mouse ads-HRP. Reactive in Rat. Host: Donkey. More details

$240 / 1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Donkey Anti-Rat IgG (H+L), Secondary Antibody
Full Product Name
Donkey Anti-Rat IgG (H+L), Mouse ads-HRP
Product Synonym Names
Donkey Anti-Rat IgG(H+L), Mouse ads - HRP; Donkey Anti-Rat IgG (H+L)
Clonality
Polyclonal
Isotype
IgG
Host
Donkey
Species Reactivity
Rat
Form/Format
Horseradish Peroxidase (HRP) Conjugate
Purity/Purification
Affinity chromatography on Rat IgG covalently linked to agarose.
Specificity
Reacts with the heavy and light chains of Rat IgG as demonstrated by ELISA. May also react with the light chains of other Rat immunoglobulins.
Preparation and Storage
The purified (UNLB) antibody is supplied as 1.0 mg purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8 degree C.
The fluorescein (FITC) conjugate is supplied as 1.0 mg in 1.0 mL PBS/NaN3. Store at 2-8 degree C.
The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mM MgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2-8 degree C or long-term at -20 degree C.
The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% PBS, pH 7.4. No preservative added. Store at 2-8 degree C or long-term at -20 degree C.
The biotin (BIOT) conjugate is supplied as 1.0 mg in 2.0 mL PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
Protect fluorochrome-conjugated forms from light. Reagents are stable for the period shown on the label if stored as directed.
Source
Pooled antisera from donkey hyperimmunized with rat IgG
Cross Absorption
Minimal cross reaction to mouse serum proteins
Characterization
To ensure lot-to-lot consistency, each batch of product is tested by ELISA and/or Fluorescent ELISA for conformance to characteristics of a standard reference reagent.
Applications Tested/Suitable for Donkey Anti-Rat IgG (H+L) secondary antibody
ELISA; Immunoblot; Immunofluorescence; Immunohistochemistry; Western Blot
ELISA Suggested Dilution: 1:4,000-1:8,000

Other Notes

Small volumes of Donkey Anti-Rat IgG (H+L) secondary antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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