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SF-20 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS235401

Rabbit anti Mouse SF-20 antibody recognizes mouse stromal cell-derived growth factor 20 (SF-20), otherwise known as interleukin-25 (IL-25), a 166 amino acid secreted growth factor and member of the UPF0556 family. SF-20 should not be confused with interleukin… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
SF-20, Polyclonal Antibody
Full Product Name
RABBIT ANTI MOUSE SF-20:Biotin
Product Gene Name
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Form/Format
Biotin
Purified IgG conjugated to Biotin - lyophilised
Preparation and Storage
Prior to reconstitution store at 4 degree C. After reconstitution store at -20 degree C. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 12 months from date of reconstitution.
Molecular Weight
17,982 Da
3D Structure
ModBase 3D Structure for Q9CPT4
Reconstitution
Reconstitute with 0.5ml sterile PBS containing 0.1% Bovine Serum Albumin. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. MyBioSource recommends that the vial is gently mixed after reconstitution. NB: For long term storage the addition of 0.09% sodium azide is recommended.
Immunogen
Recombinant mouse SF-20
Buffer Solution||Phosphate buffered saline
Antiserum Preparation
Antisera to mouse SF-20 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Target Species
Mouse
Applications Tested/Suitable for anti-SF-20 antibody
ELISA (EIA), Western Blot (WB)
Application Notes for anti-SF-20 antibody
ELISA: Minimum Dilution: 0.25; Maximum Dilution: 1.0ug/ml;
Western Blotting: Minimum Dilution: 0.1; Maximum Dilution: 0.2ug/ml

Other Notes

Small volumes of anti-SF-20 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SF-20 purchase

MBS235401 is an antibody targeting SF-20. Also known as RABBIT ANTI MOUSE SF-20:Biotin. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for SF-20. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
UPF0556 protein C19orf10 homolog
NCBI Official Synonym Full Names
myeloid derived growth factor
NCBI Official Symbol
Mydgf
NCBI Official Synonym Symbols
Il25; Ly6elg; D17Wsu104e
UniProt Protein Name
UPF0556 protein C19orf10 homolog
UniProt Synonym Protein Names
Interleukin-25; IL-25; Stromal cell-derived growth factor SF20
UniProt Gene Name
D17Wsu104e
UniProt Synonym Gene Names
Il25; IL-25
UniProt Entry Name
CS010_MOUSE
NCBI Protein Information
UPF0556 protein C19orf10 homolog

NCBI Summary for SF-20

The protein encoded by this gene was previously thought to support proliferation of lymphoid cells and was identified in error as interleukin 25. This activity has not been reproducible, however, and the function of this protein is currently unknown. [provided by RefSeq, Jul 2008]

UniProt Comments for SF-20

MYDGF: was previously thought to support proliferation of lymphoid cells and was considered an interleukin. However, this activity has not been reproducible and the function of this protein is currently unknown. [provided by RefSeq, Jul 2008]

Protein type: Secreted; Secreted, signal peptide

Cellular Component: extracellular space; extracellular region

Biological Process: positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein kinase B signaling cascade; positive regulation of angiogenesis; positive regulation of MAPKKK cascade; positive regulation of transcription from RNA polymerase II promoter; positive regulation of endothelial cell proliferation; positive regulation of protein amino acid phosphorylation; negative regulation of apoptosis
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Organs/Tissues associated with anti-SF-20 antibody

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