Anti-Nestin antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Anti-Nestin Antibody - Western Blot. Western blot of Affinity Purified anti-Nestin antibody shows detection of a band ~220 kD corresponding to human Nestin (arrowhead). Undifferentiated HCN-1A human brain cortical neuron neuronal progenitor lysate (lane 1), or differentiated HCN-1A human brain cortical neuron neuronal progenitor lysate (lane 2) were separated by SDS-PAGE using 4-20% gradient gel. After transfer onto nitrocellulose, the membrane was blocked and then probed with the primary antibody diluted to 1:2000 overnight at 4C. The membrane was then washed and reacted with a 1:10000 dilution of peroxidase conjugated affinity purified Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature. Image was captured using film. Other detection systems will yield similar results. Image courtesy of Prof. F. H. Gage of the Salk Institute, San Diego, CA.

Anti-Nestin Antibody - Western Blot. Western blot of Affinity Purified anti-Nestin antibody shows detection of a band ~220 kD corresponding to mouse Nestin (arrowhead). Approximately 30 g of MEF whole cell lysate was separated by SDS-PAGE using a 4-20% gradient gel. After transfer onto nitrocellulose, the membrane was blocked and then probed with the primary antibody diluted to 1:2000 overnight at 4C. The membrane was then washed and reacted with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX (for 45 min at room temperature. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.