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UBP43 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS241147

MBS241147 is an antibody targeting UBP43. Also known as Rabbit Polyclonal to Human UBP43 / USP18. Reactive in Human. Host: Rabbit. More details

$715 / 0.05 mg +1 FREE 8GB USB
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Sample Manual Insert View PDF
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Testing Data View PDF
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Product Overview

Online Datasheet in PDF
Product Name
UBP43 / USP18, Polyclonal Antibody
Full Product Name
Rabbit Polyclonal to Human UBP43 / USP18
Product Gene Name
Product Synonym Gene Name
USP18
Product Synonym Names
Anti-UBP43 / USP18 Antibody (aa40-49) IHC-plus; USP18; 43 kDa ISG15-specific protease; ISG43; HUBP43; ISG15 specific protease; Ubiquitin specific protease 18; Ubl thioesterase 18; Ubl thiolesterase 18; UBP43; Human UBP43; USP18
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Purity/Purification
Immunoaffinity Purified
Concentration
0.93 mg/ml (lot specific)
Specificity
Amino acids 40 to 49 of human USP18
Preparation and Storage
Long term: -20 degree C; Short term: +4 degree C. Avoid repeat freeze-thaw cycles.
Molecular Weight
41,352 Da
OMIM
607057
3D Structure
ModBase 3D Structure for Q9UMW8
Target Species
Human
Immunogen Description
Synthetic peptide from human USP18.
Immunogen Type
Synthetic peptide
Immunogen
UBP43 / USP18 antibody was raised against synthetic peptide from human USP18.
Antigen Modification
aa40-49
Applications Tested/Suitable for anti-UBP43 antibody
Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
Application Notes for anti-UBP43 antibody
ELISA (1:10000 - 1:50000), IF, IHC-P (5 ug/ml), WB (1:500 - 1:2000)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...

Immunohistochemistry (IHC) of anti-UBP43 antibody

Anti-USP18 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Rabbit Anti-UBP43 Polyclonal Antibody – Cat# MBS241147

Western Blot (WB) of anti-UBP43 antibody

Anti-UBP43 Antibody - Western Blot. Western blot of Affinity Purified anti-UBP43 antibody shows detection of a band ~43 kD band (arrowhead) believed to be to UBP43 in lysates from HeLa nuclear extracts (lane 1) and whole cell lysates from HeLa (lane 2), A431 (lane 3, Jurkat (Lane 4) and HEK293 (lane 5). Approximately 18 ug of each lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-UBP43 antibody. Signal was detected using standard techniques. The strong band between the 37kD and 50kD markers is expected to be UBP43. The other bands are probably non-specific.
Rabbit Anti-UBP43 Polyclonal Antibody, Western Blot (WB) – Cat# MBS241147

Other Notes

Small volumes of anti-UBP43 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for UBP43. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
ubl carboxyl-terminal hydrolase 18
NCBI Official Synonym Full Names
ubiquitin specific peptidase 18
NCBI Official Symbol
USP18
NCBI Official Synonym Symbols
ISG43; UBP43
UniProt Protein Name
Ubl carboxyl-terminal hydrolase 18
UniProt Synonym Protein Names
43 kDa ISG15-specific protease; hUBP43; ISG15-specific-processing protease; Ubl thioesterase 18
UniProt Gene Name
USP18
UniProt Synonym Gene Names
ISG43; hUBP43
UniProt Entry Name
UBP18_HUMAN
NCBI Protein Information
ubl carboxyl-terminal hydrolase 18; hUBP43; ubl thioesterase 18; ubl thiolesterase 18; 43 kDa ISG15-specific protease; ubiquitin specific protease 18; ISG15-specific-processing protease

NCBI Summary for UBP43

The protein encoded by this gene belongs to the ubiquitin-specific proteases (UBP) family of enzymes that cleave ubiquitin from ubiquitinated protein substrates. It is highly expressed in liver and thymus, and is localized to the nucleus. This protein efficiently cleaves only ISG15 (a ubiquitin-like protein) fusions, and deletion of this gene in mice results in a massive increase of ISG15 conjugates in tissues, indicating that this protein is a major ISG15-specific protease. Mice lacking this gene are also hypersensitive to interferon, suggesting a function of this protein in downregulating interferon responses, independent of its isopeptidase activity towards ISG15. [provided by RefSeq, Sep 2011]
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UniProt Comments for UBP43

USP18: Can efficiently cleave only ISG15 fusions including native ISG15 conjugates linked via isopeptide bonds. Necessary to maintain a critical cellular balance of ISG15-conjugated proteins in both healthy and stressed organisms. Belongs to the peptidase C19 family.

Protein type: EC 3.1.2.-; EC 3.4.19.-; Ubiquitin-specific protease; Protease

Chromosomal Location of Human Ortholog: 22q11.21

Cellular Component: cytosol; nucleus

Molecular Function: protein binding; ISG15-specific protease activity; cysteine-type endopeptidase activity; ubiquitin-specific protease activity

Biological Process: proteasomal ubiquitin-dependent protein catabolic process; protein deubiquitination; cytokine and chemokine mediated signaling pathway
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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