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MMP7 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS241179

MMP7 is a Metallopeptidase M10A type protease. MMP-7 (Matrilysin) is constitutively produced by exocrine epithelial cells through out the body. It is capable of degrading casein, fibronectin, gelatins of types 1, III, IV, and V, in addition to activating… More details

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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
MMP7, Polyclonal Antibody
Full Product Name
Rabbit Polyclonal to Human MMP7
Product Gene Name
anti-MMP7 antibody
Product Synonym Names
Anti-MMP7 Antibody (C-Terminus) IHC-plus; MMP7; Matrilysin; Matrin; MMP-7; MPSL1; PUMP-1; Pump-1 protease; MPMM; Uterine matrilysin; Punctuated metalloproteinase 1; Matrix metalloproteinase-7; PUMP1; Uterine metalloproteinase; Human MMP7
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
PBS, pH 7.4, 0.1% BSA, 0.05% sodium azide.
Purity/Purification
Immunoaffinity Purified
Concentration
0.2 mg/ml (lot specific)
Specificity
Peptide from the C-terminus of human MMP7
Preparation and Storage
Upon arrival store at 2-8 degree C for up to 1 year. Avoid repeated freeze-thaw cycles.
Molecular Weight
29,677 Da
OMIM
178990
3D Structure
ModBase 3D Structure for P09237
Target
MMP7/Matrilysin
Immunogen Species
Antibody was raised against Human
Antigen Type
Synthetic peptide
Immunogen
MMP7/Matrilysin antibody was raised against synthetic peptide from human MMP7.
Epitope
C-Terminus
Disclaimer
Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
Recommended Immunohistochemistry Protocol
The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:

Tissue Preparation:
Formalin fixation and embedding in paraffin wax.

Tissue Sectioning:
Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C.

Deparaffinization:
Wash dry slides in 3 changes of xylene - 5 minutes each @ RT

Rehydration:
Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT
Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT
Wash slides in 1 change of 80% alcohol - 3 minutes @ RT
Rinse slides in gentle running distilled water - 5 minutes @ RT

Antigen retrieval:
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) -1 minute @ RT

Immunostaining:
(Do not allow tissues to dry at any time during the staining procedure)
Apply a universal protein block - 20 minutes @ RT
Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT
Rinse slides in 1 X TBST - 1 minute @ RT
Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase streptavidin - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT
Wash slides in distilled water - 1 minute @ RT

Dehydrate:
(This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB)
Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT
Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT
Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT
Wash slides in 3 changes of xylene - 1 minute each @ RT
Apply coverslip

Product Categories/Family for anti-MMP7 antibody

Family: Protease; Subfamily: Metallopeptidase M10A
Applications Tested/Suitable for anti-MMP7 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), Immunoprecipitation (IP)
Application Notes for anti-MMP7 antibody
IHC-P (10 ug/ml)
IP (2 - 5 ng/10E6 cells)
WB (0.5 - 2 ug/ml)

Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for MBS241179 was determined to be 10 ug/ml.

Immunohistochemistry (IHC) of anti-MMP7 antibody

Anti-MMP7 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Rabbit Anti-MMP7 Polyclonal Antibody – Cat# MBS241179

Other Notes

Small volumes of anti-MMP7 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MMP7 purchase

MBS241179 is an antibody targeting MMP7. Also known as Rabbit Polyclonal to Human MMP7. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for MMP7. It may not necessarily be applicable to this product.

NCBI GI #
4505219
NCBI GeneID
4316
NCBI Accession #
NP_002414.1
UniProt Primary Accession #
P09237
UniProt Secondary Accession #
Q9BTK9
UniProt Related Accession #
P09237
GenBank Nucleotide #
NM_002423.3
NCBI Official Full Name
matrilysin preproprotein
NCBI Official Synonym Full Names
matrix metallopeptidase 7 (matrilysin, uterine)
NCBI Official Symbol
MMP7
NCBI Official Synonym Symbols
MMP-7; MPSL1; PUMP-1
UniProt Protein Name
Matrilysin
UniProt Synonym Protein Names
Matrin; Matrix metalloproteinase-7; MMP-7; Pump-1 protease; Uterine metalloproteinase
Protein Family
Matrilysin
UniProt Gene Name
MMP7
UniProt Synonym Gene Names
MPSL1; PUMP1; MMP-7
UniProt Entry Name
MMP7_HUMAN
NCBI Protein Information
matrilysin; matrin; pump-1 protease; uterine matrilysin; uterine metalloproteinase; matrix metalloproteinase-7; matrix metalloproteinase 7 (matrilysin, uterine)

NCBI Summary for MMP7

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades proteoglycans, fibronectin, elastin and casein and differs from most MMP family members in that it lacks a conserved C-terminal protein domain. The enzyme is involved in wound healing, and studies in mice suggest that it regulates the activity of defensins in intestinal mucosa. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. [provided by RefSeq, Jul 2008]
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UniProt Comments for MMP7

MMP7: Degrades casein, gelatins of types I, III, IV, and V, and fibronectin. Activates procollagenase. Belongs to the peptidase M10A family.

Protein type: Protease; Secreted, signal peptide; Secreted; EC 3.4.24.23

Chromosomal Location of Human Ortholog: 11q22.2

Cellular Component: proteinaceous extracellular matrix; extracellular space; extracellular region

Molecular Function: zinc ion binding; metalloendopeptidase activity

Biological Process: response to drug; extracellular matrix disassembly; collagen catabolic process; defense response to Gram-positive bacterium; extracellular matrix organization and biogenesis; antibacterial peptide biosynthetic process; proteolysis; defense response to Gram-negative bacterium; antibacterial peptide secretion; regulation of cell proliferation
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Research Articles on MMP7

1. The results showed that MMP7-181A/G and MMP13-77A/G variants were associated with Colorectal cancer

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-MMP7 antibody

 Reference Product  PubMed Publications
 MMP1 antibody  >62 publications with MMP7 and MMP1
 CTNNB1 antibody  >58 publications with MMP7 and CTNNB1
 TIMP1 antibody  >33 publications with MMP7 and TIMP1
 CD44 antibody  >21 publications with MMP7 and CD44
 HBEGF antibody  >7 publications with MMP7 and HBEGF
 COL18A1 antibody  >3 publications with MMP7 and COL18A1
 SDC1 antibody  >2 publications with MMP7 and SDC1
 NGF antibody  >1 publications with MMP7 and NGF

Pathways associated with anti-MMP7 antibody

 Products by Pathway  Pathway Diagram
 AGE/RAGE Pathway antibodies  AGE/RAGE Pathway Diagram
 Activation Of Matrix Metalloproteinases Pathway antibodies  Activation Of Matrix Metalloproteinases Pathway Diagram
 Alpha6-Beta4 Integrin Signaling Pathway antibodies  Alpha6-Beta4 Integrin Signaling Pathway Diagram
 Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway antibodies  Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway Diagram
 Collagen Degradation Pathway antibodies  Collagen Degradation Pathway Diagram
 Collagen Formation Pathway antibodies  Collagen Formation Pathway Diagram
 Degradation Of The Extracellular Matrix Pathway antibodies  Degradation Of The Extracellular Matrix Pathway Diagram
 Extracellular Matrix Organization Pathway antibodies  Extracellular Matrix Organization Pathway Diagram
 Matrix Metalloproteinases Pathway antibodies  Matrix Metalloproteinases Pathway Diagram
 Syndecan-1-mediated Signaling Events Pathway antibodies  Syndecan-1-mediated Signaling Events Pathway Diagram

Diseases associated with anti-MMP7 antibody

 Disease Name  Pubmed Publications
 Neoplasm Metastasis Antibodies  >89 publications with MMP7 and Neoplasm Metastasis
 Adenocarcinoma Antibodies  >88 publications with MMP7 and Adenocarcinoma
 Neoplasm Invasiveness Antibodies  >75 publications with MMP7 and Neoplasm Invasiveness
 Lung Diseases Antibodies  >60 publications with MMP7 and Lung Diseases
 Inflammation Antibodies  >56 publications with MMP7 and Inflammation
 Lung Neoplasms Antibodies  >40 publications with MMP7 and Lung Neoplasms
 Fibrosis Antibodies  >35 publications with MMP7 and Fibrosis
 Colonic Neoplasms Antibodies  >31 publications with MMP7 and Colonic Neoplasms
 Disease Models, Animal Antibodies  >30 publications with MMP7 and Disease Models, Animal
 Breast Neoplasms Antibodies  >29 publications with MMP7 and Breast Neoplasms

Organs/Tissues associated with anti-MMP7 antibody

 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >83 publications with MMP7 and Lung
 Liver Antibodies  >33 publications with MMP7 and Liver
 Stomach Antibodies  >29 publications with MMP7 and Stomach
 Intestine Antibodies  >28 publications with MMP7 and Intestine
 Kidney Antibodies  >28 publications with MMP7 and Kidney
 Brain Antibodies  >23 publications with MMP7 and Brain
 Skin Antibodies  >17 publications with MMP7 and Skin
 Prostate Antibodies  >15 publications with MMP7 and Prostate
 Uterus Antibodies  >15 publications with MMP7 and Uterus
 Connective Tissue Antibodies  >14 publications with MMP7 and Connective Tissue
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