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5-Alpha Reductase / SRD5A1 (SRD5A1) Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS241657

MBS241657 is an antibody targeting SRD5A1. Also known as Goat Polyclonal to Human 5-Alpha Reductase / SRD5A1. Reactive in Gibbon, HumanPredicted Reactivity: Gorilla, Monkey (at least 90% immunogen sequence identity). Host: Goat. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
5-Alpha Reductase / SRD5A1, Polyclonal Antibody
Full Product Name
Goat Polyclonal to Human 5-Alpha Reductase / SRD5A1
Product Gene Name
Product Synonym Names
Anti-5-Alpha Reductase / SRD5A1 Antibody (N-Terminus) IHC-plus; SRD5A1; 5-alpha reductase; SR type 1; Steroid 5-alpha-reductase 1; S5AR 1; Human 5-Alpha Reductase; SRD5A1
Clonality
Polyclonal
Host
Goat
Species Reactivity
Gibbon, Human
Predicted Reactivity: Gorilla, Monkey (at least 90% immunogen sequence identity)
Form/Format
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Purity/Purification
Immunoaffinity Purified
Concentration
0.5 mg/ml (lot specific)
Specificity
Human SRD5A1.
Preparation and Storage
Store at -20 degree C. Minimize freezing and thawing.
Molecular Weight
29,459 Da
OMIM
184753
3D Structure
ModBase 3D Structure for P18405
Target Species
Human
Immunogen Description
Synthetic peptide ATATGVAEERLLC from the N-terminus of human SRD5A1 (NP_001038.1). Percent identity by BLAST analysis: Human, Gibbon (100%); Gorilla, Marmoset (92%); Ajellomyces (83%).
Immunogen Type
Synthetic peptide
Immunogen
5-Alpha Reductase / SRD5A1 antibody was raised against synthetic peptide ATATGVAEERLLC from the N-terminus of human SRD5A1 (NP_001038.1). Percent identity by BLAST analysis: Human, Gibbon (100%); Gorilla, Marmoset (92%); Ajellomyces (83%).
Antigen Modification
N-Terminus
Applications Tested/Suitable for anti-SRD5A1 antibody
Immunohistochemistry (IHC - Paraffin), ELISA (EIA)
Application Notes for anti-SRD5A1 antibody
ELISA (1:64000), IHC-P (2.5 ug/ml)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...

Immunohistochemistry (IHC) of anti-SRD5A1 antibody

Anti-SRD5A1 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Goat Anti-SRD5A1 Polyclonal Antibody – Cat# MBS241657

Other Notes

Small volumes of anti-SRD5A1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for SRD5A1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
3-oxo-5-alpha-steroid 4-dehydrogenase 1
NCBI Official Synonym Full Names
steroid-5-alpha-reductase, alpha polypeptide 1 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 1)
NCBI Official Symbol
SRD5A1
NCBI Official Synonym Symbols
S5AR 1
UniProt Protein Name
3-oxo-5-alpha-steroid 4-dehydrogenase 1
UniProt Synonym Protein Names
SR type 1; Steroid 5-alpha-reductase 1; S5AR 1
UniProt Gene Name
SRD5A1
UniProt Synonym Gene Names
S5AR 1
UniProt Entry Name
S5A1_HUMAN
NCBI Protein Information
3-oxo-5-alpha-steroid 4-dehydrogenase 1; SR type 1; 5-alpha reductase; steroid 5-alpha-reductase 1; steroid 5-alpha-reductase type I; 3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 1

NCBI Summary for SRD5A1

Steroid 5-alpha-reductase (EC 1.3.99.5) catalyzes the conversion of testosterone into the more potent androgen, dihydrotestosterone (DHT). Also see SRD5A2 (MIM 607306).[supplied by OMIM, Mar 2008]

UniProt Comments for SRD5A1

SRD5A1: Converts testosterone into 5-alpha-dihydrotestosterone and progesterone or corticosterone into their corresponding 5- alpha-3-oxosteroids. It plays a central role in sexual differentiation and androgen physiology. Belongs to the steroid 5-alpha reductase family.

Protein type: Lipid Metabolism - androgen and estrogen; Membrane protein, integral; Oxidoreductase; Membrane protein, multi-pass; EC 1.3.1.22

Chromosomal Location of Human Ortholog: 5p15

Cellular Component: endoplasmic reticulum membrane; integral to membrane

Molecular Function: electron carrier activity; cholestenone 5-alpha-reductase activity; 3-oxo-5-alpha-steroid 4-dehydrogenase activity

Biological Process: steroid metabolic process; sex determination; androgen biosynthetic process; cell differentiation; sex differentiation
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Precautions

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Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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