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Histone H2B (HTB2) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS241838

MBS241838 is an antibody targeting HTB2. Also known as Rabbit Polyclonal (IgG) to Human Histone H2B. Reactive in Bovine, Chicken, Drosophila, Human, Insect, Mouse, Orangutan, Rat, Zebrafish. Host: Rabbit. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Product Name
Histone H2B (HTB2), Polyclonal Antibody
Full Product Name
Rabbit Polyclonal (IgG) to Human Histone H2B
Product Gene Name
Product Synonym Names
Anti-Histone H2B Antibody (aa111-125) IHC-plus; Histone H2B; Human Histone H2B
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Bovine, Chicken, Drosophila, Human, Insect, Mouse, Orangutan, Rat, Zebrafish
Form/Format
PBS, 0.05% BSA, 0.05% sodium azide
Purity/Purification
Protein G Purified
Concentration
1 mg/ml (lot specific)
Specificity
A synthetic peptide corresponding to amino acid residues 111-125 AVSEGTKAVTKYTSSof human Histone H2B; GenBank no. CAI19747.1. This peptide sequence is 100% conserved between multiple species including human, mouse, rat, dog, chimpanzee, cow, chicken.
Preparation and Storage
Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.
Molecular Weight
15,732 Da
Target Species
Human
Immunogen Description
This antibody was generated by immunizing rabbits with a synthetic peptide corresponding to amino acid residues 111-125 AVSEGTKAVTKYTSSof human Histone H2B; GenBank no. CAI19747.1.
Immunogen Type
Synthetic peptide
Immunogen
Histone H2B antibody was raised against this antibody was generated by immunizing rabbits with a synthetic peptide corresponding to amino acid residues 111-125 AVSEGTKAVTKYTSSof human Histone H2B; GenBank no. CAI19747.1. This peptide sequence is 100% conserved between multiple species including human, mouse, rat, dog, chimpanzee, cow, chicken, duck, zebrafish, and drosophila. Percent identity by BLAST analysis: Human, Orangutan, Mouse, Rat, Bovine, Chicken, Stickleback, Pufferfish, Zebrafish, Drosophila, Mosquito, Nematode (100%).
Antigen Modification
aa111-125
Applications Tested/Suitable for anti-HTB2 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
Application Notes for anti-HTB2 antibody
IHC-P (5 ug/ml), WB (2 ug/ml)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...

Immunohistochemistry (IHC) of anti-HTB2 antibody

Anti-Histone H2B antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Rabbit Anti-HTB2 Polyclonal Antibody – Cat# MBS241838

Western Blot (WB) of anti-HTB2 antibody

Western blot of fractionated Cos-7 cells using antibody anti-Histone H2B.? Cos-7 cells were fractionated into nuclear & cytosolic/membrane fractions. Approximately ~15 ug of each fraction was?separated on SDS PAGE.?? Lane 1, Nuclear fraction.ulane
Rabbit Anti-HTB2 Polyclonal Antibody, Western Blot (WB) – Cat# MBS241838

Western Blot (WB) of anti-HTB2 antibody

Western blot of Histone H2B in human PBMC lysate with antibody.
Rabbit Anti-HTB2 Polyclonal Antibody, Western Blot (WB) – Cat# MBS241838

Western Blot (WB) of anti-HTB2 antibody

Western blot of Histone H2B using antibody at 1:250 in nuclear and cytoplasmic fractionsumade from Drosophila head extracts.
Rabbit Anti-HTB2 Polyclonal Antibody, Western Blot (WB) – Cat# MBS241838

Other Notes

Small volumes of anti-HTB2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for HTB2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
histone H2B
NCBI Official Symbol
HTB2
NCBI Official Synonym Symbols
H2B; histone B2; HISTONE H2B; HTB2; MRN17.11; MRN17_11
UniProt Protein Name
Histone H2B.10
UniProt Synonym Protein Names
HTB2
UniProt Gene Name
At5g22880
UniProt Entry Name
H2B10_ARATH
NCBI Protein Information
histone H2B

UniProt Comments for HTB2

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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