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APOD Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS241904

Apolipoprotein D (Apo-D) is a component of high density lipoprotein that has no marked similarity to other apolipoprotein sequences. It has a high degree of homology to plasma retinol-binding protein and other members of the alpha 2 microglobulin protein… More details

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Product Overview

Online Datasheet in PDF
Product Name
APOD / Apolipoprotein D, Polyclonal Antibody
Full Product Name
Rabbit Polyclonal to Mouse APOD / Apolipoprotein D
Product Gene Name
anti-APOD antibody
Product Synonym Names
APOD Antibody, Apo-D Antibody, Apolipoprotein D Antibody
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
PBS, 0.01% sodium azide, 50% glycerol.
Purity/Purification
Immunoaffinity Purified
Concentration
0.5 mg/ml (lot specific)
Specificity
Synthetic peptide corresponding to N-terminal residues of mouse APOD(Apolipoprotein D precursor).
Preparation and Storage
Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.
Molecular Weight
21,276 Da
OMIM
107740
3D Structure
ModBase 3D Structure for P05090
Target
Mouse APOD / Apolipoprotein D
Target Species
Mouse
Immunogen Description
Synthetic peptide from mouse APOD / Apolipoprotein D.
Immunogen Type
Synthetic peptide
Immunogen
APOD / Apolipoprotein D antibody was raised against synthetic peptide from mouse APOD / Apolipoprotein D.
Antigen Modification
N-Terminus
Disclaimer
Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
Recommended Immunohistochemistry Protocol
The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:

Tissue Preparation:
Formalin fixation and embedding in paraffin wax.

Tissue Sectioning:
Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C.

Deparaffinization:
Wash dry slides in 3 changes of xylene - 5 minutes each @ RT

Rehydration:
Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT
Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT
Wash slides in 1 change of 80% alcohol - 3 minutes @ RT
Rinse slides in gentle running distilled water - 5 minutes @ RT

Antigen retrieval:
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) -1 minute @ RT

Immunostaining:
(Do not allow tissues to dry at any time during the staining procedure)
Apply a universal protein block - 20 minutes @ RT
Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT
Rinse slides in 1 X TBST - 1 minute @ RT
Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase streptavidin - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT
Wash slides in distilled water - 1 minute @ RT

Dehydrate:
(This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB)
Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT
Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT
Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT
Wash slides in 3 changes of xylene - 1 minute each @ RT
Apply coverslip
Applications Tested/Suitable for anti-APOD antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
Application Notes for anti-APOD antibody
IHC-P (10 ug/ml), WB (1 ug/ml)

Usage:
Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for MBS241904 was determined to be 10 ug/ml.

Immunohistochemistry (IHC) of anti-APOD antibody

Anti-APOD / Apolipoprotein D antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Rabbit Anti-APOD Polyclonal Antibody – Cat# MBS241904

Other Notes

Small volumes of anti-APOD antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for APOD purchase

MBS241904 is an antibody targeting APOD. Also known as Rabbit Polyclonal to Mouse APOD / Apolipoprotein D. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for APOD. It may not necessarily be applicable to this product.

NCBI GI #
4502163
NCBI GeneID
347
NCBI Accession #
NP_001638.1
UniProt Primary Accession #
P05090
UniProt Secondary Accession #
Q6IBG6; B2R579; D3DNW6
UniProt Related Accession #
P05090
GenBank Nucleotide #
NM_001647.3
NCBI Official Full Name
apolipoprotein D
NCBI Official Synonym Full Names
apolipoprotein D
NCBI Official Symbol
APOD
UniProt Protein Name
Apolipoprotein D
Protein Family
Apolipoprotein
UniProt Gene Name
APOD
UniProt Synonym Gene Names
Apo-D; ApoD
UniProt Entry Name
APOD_HUMAN
NCBI Protein Information
apolipoprotein D; apo-D

NCBI Summary for APOD

This gene encodes a component of high density lipoprotein that has no marked similarity to other apolipoprotein sequences. It has a high degree of homology to plasma retinol-binding protein and other members of the alpha 2 microglobulin protein superfamily of carrier proteins, also known as lipocalins. This glycoprotein is closely associated with the enzyme lecithin:cholesterol acyltransferase - an enzyme involved in lipoprotein metabolism. [provided by RefSeq, Aug 2008]
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UniProt Comments for APOD

APOD: APOD occurs in the macromolecular complex with lecithin- cholesterol acyltransferase. It is probably involved in the transport and binding of bilin. Appears to be able to transport a variety of ligands in a number of different contexts. Belongs to the calycin superfamily. Lipocalin family.

Protein type: Secreted, signal peptide; Lipid-binding; Secreted

Chromosomal Location of Human Ortholog: 3q29

Cellular Component: extracellular space; cell soma; endoplasmic reticulum; perinuclear region of cytoplasm; dendrite; extracellular region

Molecular Function: lipid transporter activity; protein binding; cholesterol binding

Biological Process: response to drug; negative regulation of smooth muscle cell proliferation; negative regulation of focal adhesion formation; glucose metabolic process; negative regulation of protein import into nucleus; lipid transport; axon regeneration in the peripheral nervous system; response to reactive oxygen species; tissue regeneration; brain development; lipid metabolic process; response to axon injury; angiogenesis; aging
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Research Articles on APOD

1. ApoE is located in the nucleus and on the ApoD promoter in human hepatic and glioblastoma cells lines.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-APOD antibody

 Reference Product  PubMed Publications
 LEPR antibody  >2 publications with APOD and LEPR

Pathways associated with anti-APOD antibody

 Products by Pathway  Pathway Diagram
 Orphan Transporters Pathway antibodies  Orphan Transporters Pathway Diagram
 SLC-mediated Transmembrane Transport Pathway antibodies  SLC-mediated Transmembrane Transport Pathway Diagram
 Transmembrane Transport Of Small Molecules Pathway antibodies  Transmembrane Transport Of Small Molecules Pathway Diagram
 Transport Of Fatty Acids Pathway antibodies  Transport Of Fatty Acids Pathway Diagram
 Transport Of Vitamins, Nucleosides, And Related Molecules Pathway antibodies  Transport Of Vitamins, Nucleosides, And Related Molecules Pathway Diagram

Diseases associated with anti-APOD antibody

 Disease Name  Pubmed Publications
 Skin Diseases Antibodies  >191 publications with APOD and Skin Diseases
 Breast Neoplasms Antibodies  >155 publications with APOD and Breast Neoplasms
 Carcinoma Antibodies  >124 publications with APOD and Carcinoma
 Adenocarcinoma Antibodies  >67 publications with APOD and Adenocarcinoma
 Nervous System Diseases Antibodies  >50 publications with APOD and Nervous System Diseases
 Skin Neoplasms Antibodies  >44 publications with APOD and Skin Neoplasms
 Brain Diseases Antibodies  >39 publications with APOD and Brain Diseases
 Neurodegenerative Diseases Antibodies  >27 publications with APOD and Neurodegenerative Diseases
 Cardiovascular Diseases Antibodies  >15 publications with APOD and Cardiovascular Diseases
 Metaplasia Antibodies  >15 publications with APOD and Metaplasia

Organs/Tissues associated with anti-APOD antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >78 publications with APOD and Brain
 Skin Antibodies  >70 publications with APOD and Skin
 Nerve Antibodies  >29 publications with APOD and Nerve
 Liver Antibodies  >27 publications with APOD and Liver
 Mammary Gland Antibodies  >23 publications with APOD and Mammary Gland
 Muscle Antibodies  >21 publications with APOD and Muscle
 Prostate Antibodies  >18 publications with APOD and Prostate
 Vascular Antibodies  >17 publications with APOD and Vascular
 Lung Antibodies  >16 publications with APOD and Lung
 Lymph Node Antibodies  >14 publications with APOD and Lymph Node
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