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CXCR6 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS242091

Coreceptor usage of primary human immunodeficiency virus type 1 (HIV-1) isolates varies according to biological phenotype. The chemokine receptors CCR5 and CXCR4 are the major coreceptors that, together with CD4, govern HIV-1 entry into cells. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
CXCR6, Polyclonal Antibody
Full Product Name
Rabbit Polyclonal to Human CXCR6
Product Gene Name
anti-CXCR6 antibody
Product Synonym Names
Anti-CXCR6 Antibody (N-Terminus) IHC-plus; CXCR6; CD186 antigen; CXCR-6; STRL33; TYMSTR; BONZO; CD186; CDw186; CXC-R6; Human CXCR6
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
PBS, 0.02% sodium azide.
Purity/Purification
Peptide Column Affinity Chromatography
Concentration
1 mg/ml (lot specific)
Specificity
peptide corresponding to amino acids near the amino terminus of human Bonzo/STRL33 The sequence of this peptide differs from those of African green monkey and pig-tailed macaque by one or two amino acids, respectively,
Preparation and Storage
Store at 4°C for up 3 months. Store at -20°C for up to 1 year. Avoid freeze/thaw cycles.
Molecular Weight
Western Blot:
Predicted: 38 kDa Observed: 43 kDa
OMIM
605163
3D Structure
ModBase 3D Structure for O00574
Immunogen Species
Human
Antigen Type
Synthetic peptide
Immunogen
CXCR6 antibody was raised against a peptide corresponding to 15 amino avids near the amino terminus of human Bonzo/STRL33. The immunogen is located within the first 50 amino acids of Bonzo.
Disclaimer
Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
Recommended Immunohistochemistry Protocol
The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:

Tissue Preparation:
Formalin fixation and embedding in paraffin wax.

Tissue Sectioning:
Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C.

Deparaffinization:
Wash dry slides in 3 changes of xylene - 5 minutes each @ RT

Rehydration:
Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT
Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT
Wash slides in 1 change of 80% alcohol - 3 minutes @ RT
Rinse slides in gentle running distilled water - 5 minutes @ RT

Antigen retrieval:
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) -1 minute @ RT

Immunostaining:
(Do not allow tissues to dry at any time during the staining procedure)
Apply a universal protein block - 20 minutes @ RT
Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT
Rinse slides in 1 X TBST - 1 minute @ RT
Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase streptavidin - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT
Wash slides in distilled water - 1 minute @ RT

Dehydrate:
(This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB)
Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT
Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT
Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT
Wash slides in 3 changes of xylene - 1 minute each @ RT
Apply coverslip

Product Categories/Family for anti-CXCR6 antibody

Family: GPCR; Subfamily: Chemokine
Applications Tested/Suitable for anti-CXCR6 antibody
Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA
Application Notes for anti-CXCR6 antibody
IHC-P: (2.5 ug/ml)
WB: (1:500)
Usage: Bonzo antibody can be used for detection of Bonzo by Western blot at 0.5 ug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 ug/mL. For Immunofluorescence start at 20 ug/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Immunohistochemistry (IHC) of anti-CXCR6 antibody

Anti-CXCR6 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Rabbit Anti-CXCR6 Polyclonal Antibody – Cat# MBS242091

Western Blot (WB) of anti-CXCR6 antibody

Western blot of Bonzo in human spleen tissue lysate in the absence (lane A) or presence (lane B) of peptide with Bonzo antibody at 1 ug/ml.
Rabbit Anti-CXCR6 Polyclonal Antibody, Western Blot (WB) – Cat# MBS242091

Other Notes

Small volumes of anti-CXCR6 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CXCR6 purchase

MBS242091 is an antibody targeting CXCR6. Also known as Rabbit Polyclonal to Human CXCR6. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for CXCR6. It may not necessarily be applicable to this product.

NCBI GI #
5730106
NCBI GeneID
10663
NCBI Accession #
NP_006555.1
UniProt Primary Accession #
O00574
UniProt Secondary Accession #
O00575; Q9HCA5
UniProt Related Accession #
O00574
GenBank Nucleotide #
NM_006564.1
NCBI Official Full Name
C-X-C chemokine receptor type 6
NCBI Official Synonym Full Names
chemokine (C-X-C motif) receptor 6
NCBI Official Symbol
CXCR6
NCBI Official Synonym Symbols
BONZO; CD186; STRL33; TYMSTR
UniProt Protein Name
C-X-C chemokine receptor type 6
UniProt Synonym Protein Names
CDw186; G-protein coupled receptor STRL33; G-protein coupled receptor bonzo; CD_antigen: CD186
Protein Family
C-X-C chemokine receptor
UniProt Gene Name
CXCR6
UniProt Synonym Gene Names
BONZO; STRL33; TYMSTR; CXC-R6; CXCR-6
UniProt Entry Name
CXCR6_HUMAN
NCBI Protein Information
C-X-C chemokine receptor type 6; CDw186; CXC-R6; CXCR-6; G protein-coupled receptor; G-protein coupled receptor bonzo; G-protein coupled receptor STRL33

UniProt Comments for CXCR6

CXCR6: Receptor for the C-X-C chemokine CXCL16. Used as a coreceptor by SIVs and by strains of HIV-2 and m-tropic HIV-1. Belongs to the G-protein coupled receptor 1 family.

Protein type: Receptor, GPCR; Membrane protein, integral; Membrane protein, multi-pass; GPCR, family 1

Chromosomal Location of Human Ortholog: 3p21

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: G-protein coupled receptor activity; coreceptor activity; C-X-C chemokine receptor activity

Biological Process: G-protein coupled receptor protein signaling pathway; viral genome replication; inflammatory response; chemotaxis
Show more

Research Articles on CXCR6

1. CXCL16/CXCR6 interaction may play an important role in modifying the response of pDCs to environmental danger signals

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CXCR6 antibody

 Reference Product  PubMed Publications
 CXCL16 antibody  >147 publications with CXCR6 and CXCL16
 CCR5 antibody  >84 publications with CXCR6 and CCR5
 CXCL10 antibody  >19 publications with CXCR6 and CXCL10
 CCL5 antibody  >17 publications with CXCR6 and CCL5
 CCL20 antibody  >12 publications with CXCR6 and CCL20
 CXCL9 antibody  >11 publications with CXCR6 and CXCL9
 CXCL13 antibody  >6 publications with CXCR6 and CXCL13
 CCL21 antibody  >2 publications with CXCR6 and CCL21
 CCL19 antibody  >2 publications with CXCR6 and CCL19
 CXCL11 antibody  >2 publications with CXCR6 and CXCL11

Pathways associated with anti-CXCR6 antibody

 Products by Pathway  Pathway Diagram
 Chemokine Receptors Bind Chemokines Pathway antibodies  Chemokine Receptors Bind Chemokines Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway antibodies  Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 G Alpha (i) Signalling Events Pathway antibodies  G Alpha (i) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram

Diseases associated with anti-CXCR6 antibody

 Disease Name  Pubmed Publications
 Lung Diseases Antibodies  >17 publications with CXCR6 and Lung Diseases
 Nervous System Diseases Antibodies  >15 publications with CXCR6 and Nervous System Diseases
 Skin Diseases Antibodies  >10 publications with CXCR6 and Skin Diseases
 Brain Diseases Antibodies  >10 publications with CXCR6 and Brain Diseases
 Prostatic Neoplasms Antibodies  >9 publications with CXCR6 and Prostatic Neoplasms
 Liver Neoplasms Antibodies  >8 publications with CXCR6 and Liver Neoplasms
 Breast Neoplasms Antibodies  >8 publications with CXCR6 and Breast Neoplasms
 Neoplasms, Experimental Antibodies  >7 publications with CXCR6 and Neoplasms, Experimental
 Demyelinating Diseases Antibodies  >7 publications with CXCR6 and Demyelinating Diseases
 Lymphoma Antibodies  >7 publications with CXCR6 and Lymphoma

Organs/Tissues associated with anti-CXCR6 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >13 publications with CXCR6 and Brain
 Kidney Antibodies  >13 publications with CXCR6 and Kidney
 Spleen Antibodies  >12 publications with CXCR6 and Spleen
 Placenta Antibodies  >8 publications with CXCR6 and Placenta
 Uterus Antibodies  >2 publications with CXCR6 and Uterus
 Mammary Gland Antibodies  >1 publications with CXCR6 and Mammary Gland
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