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ATG16L1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS242450

MBS242450 is an antibody targeting ATG16L1. Also known as Rabbit Polyclonal to Human ATG16L1 / ATG16L. Reactive in Human, Mouse, Rat. Host: Rabbit. More details

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Product Overview

Product Name
ATG16L1 / ATG16L, Polyclonal Antibody
Full Product Name
Rabbit Polyclonal to Human ATG16L1 / ATG16L
Product Gene Name
Product Synonym Gene Name
ATG16L
Product Synonym Names
Anti-ATG16L1 / ATG16L Antibody (N-Terminus) IHC-plus; ATG16L1; APG16-like 1; APG16L; APG16L beta; ATG16L; Autophagy-related protein 16-1; IBD10; WD repeat domain 30; ATG16A; WDR30; Human ATG16L1; ATG16L
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
PBS, 0.02% sodium azide.
Purity/Purification
Immunoaffinity Purified
Concentration
1 mg/ml (lot specific)
Preparation and Storage
Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles. Store undiluted.
Molecular Weight
49,486 Da
OMIM
gene 611081
3D Structure
ModBase 3D Structure for Q676U5
Target Species
Human
Immunogen Description
18 amino acid peptide from near the amino terminus of human ATG16.
Immunogen
ATG16L1 / ATG16L antibody was raised against 18 amino acid peptide from near the amino terminus of human ATG16.
Antigen Modification
N-Terminus
Applications Tested/Suitable for anti-ATG16L1 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes for anti-ATG16L1 antibody
ELISA, IHC-P (5 ug/ml), WB (0.5 - 1 ug/ml)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...

Immunohistochemistry (IHC) of anti-ATG16L1 antibody

Anti-ATG16L1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Rabbit Anti-ATG16L1 Polyclonal Antibody – Cat# MBS242450

Immunohistochemistry (IHC) of anti-ATG16L1 antibody

Anti-ATG16L1 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Rabbit Anti-ATG16L1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS242450

Immunofluorescence (IF) of anti-ATG16L1 antibody

Immunofluorescence of ATG16 in HeLa cells with ATG16 antibody at 4.75 ug/ml.
Rabbit Anti-ATG16L1 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS242450

Immunocytochemistry (ICC) of anti-ATG16L1 antibody

Immunocytochemistry of ATG16 in HeLa cells with ATG16 antibody at 2 ug/ml.
Rabbit Anti-ATG16L1 Polyclonal Antibody, Immunocytochemistry (ICC) – Cat# MBS242450

Western Blot (WB) of anti-ATG16L1 antibody

Western blot of ATG16 in HeLa cell lysate with ATG16 antibody at (A) 1 and (B) 2 ug/ml.
Rabbit Anti-ATG16L1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS242450

Other Notes

Small volumes of anti-ATG16L1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ATG16L1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
autophagy-related protein 16-1 isoform 2
NCBI Official Synonym Full Names
autophagy related 16-like 1 (S. cerevisiae)
NCBI Official Symbol
ATG16L1
NCBI Official Synonym Symbols
IBD10; WDR30; APG16L; ATG16A; ATG16L
UniProt Protein Name
Autophagy-related protein 16-1
UniProt Synonym Protein Names
APG16-like 1
UniProt Gene Name
ATG16L1
UniProt Synonym Gene Names
APG16L
UniProt Entry Name
A16L1_HUMAN
NCBI Protein Information
autophagy-related protein 16-1; APG16L beta; WD repeat domain 30; ATG16 autophagy related 16-like 1

NCBI Summary for ATG16L1

The protein encoded by this gene is part of a large protein complex that is necessary for autophagy, the major process by which intracellular components are targeted to lysosomes for degradation. Defects in this gene are a cause of susceptibility to inflammatory bowel disease type 10 (IBD10). Several transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jun 2010]
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UniProt Comments for ATG16L1

ATG16: Plays an essential role in autophagy. Homooligomer. Interacts with ATG5. Part of either the minor and major complexes respectively composed of 4 sets of ATG12-ATG5 and ATG16L1 (400 kDa) or 8 sets of ATG12-ATG5 and ATG16L1 (800 kDa). Belongs to the WD repeat ATG16 family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold; Autophagy; Membrane protein, peripheral

Chromosomal Location of Human Ortholog: 2q37.1

Cellular Component: pre-autophagosomal structure membrane; autophagic vacuole; axoneme

Molecular Function: identical protein binding; protein binding

Biological Process: protein transport; protein homooligomerization; autophagic vacuole formation

Disease: Inflammatory Bowel Disease 10
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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