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NT5E Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS242461

MBS242461 is an antibody targeting NT5E. Also known as Rabbit Polyclonal to Human NT5E / eNT / CD73. Reactive in Human, Mouse. Host: Rabbit. More details

$650 / 0.2 mL +1 FREE 8GB USB
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Testing Data View PDF
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Product Overview

Online Datasheet in PDF
Product Name
NT5E / eNT / CD73, Polyclonal Antibody
Full Product Name
Rabbit Polyclonal to Human NT5E / eNT / CD73
Product Gene Name
anti-NT5E antibody
Product Synonym Gene Name
eNT; CD73
Product Synonym Names
Anti-NT5E / eNT / CD73 Antibody (C-Terminus) IHC-plus; NT5E; 5-nucleotidase; ecto (CD73); 5'-nucleotidase; 5 nucleotidase (CD73); 5-NT; 5-nucleotidase; 5'-nucleotidase; ecto (CD73); CD73; CD73 antigen; E5NT; Ecto-5-nucleotidase; ENT; EN; NT; Purine 5-Prime-Nucleotidase; NT5; NTE; Human NT5E; eNT; CD73
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
PBS, 0.09% sodium azide
Purity/Purification
Ammonium sulfate precipitation
Specificity
Cross-reactivity with mouse has been confirmed in WB only. The antibody has not been evaluated for use in IHC with mouse tissues at time.
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.
Molecular Weight
57,949 Da
OMIM
gene 211800
3D Structure
ModBase 3D Structure for P21589
Target Species
Human
Immunogen Description
KLH conjugated synthetic peptide selected from the C-terminal region of human NT5E.
Immunogen Type
Synthetic peptide
Immunogen
NT5E / eNT / CD73 antibody was raised against kLH conjugated synthetic peptide selected from the C-terminal region of human NT5E.
Antigen Modification
C-Terminus
Applications Tested/Suitable for anti-NT5E antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
Application Notes for anti-NT5E antibody
IHC-P (10 ug/ml), WB (1:1000)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...

Immunohistochemistry (IHC) of anti-NT5E antibody

Anti-NT5E / CD73 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Rabbit Anti-NT5E Polyclonal Antibody – Cat# MBS242461

Immunohistochemistry (IHC) of anti-NT5E antibody

Formalin-fixed and paraffin-embedded human Placenta tissue reacted with CD73 (NT5E) Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Rabbit Anti-NT5E Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS242461

Western Blot (WB) of anti-NT5E antibody

Western blot of mouse brain tissue lysate.
Rabbit Anti-NT5E Polyclonal Antibody, Western Blot (WB) – Cat# MBS242461

Western Blot (WB) of anti-NT5E antibody

Western blot analysis of CD73 Pab in mouse brain tissue lysate. CD73 (Arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Rabbit Anti-NT5E Polyclonal Antibody, Western Blot (WB) – Cat# MBS242461

Western Blot (WB) of anti-NT5E antibody

Western blot analysis of NT5E (arrow) using rabbit polyclonal NT5E Antibody (M535). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the NT5E gene.
Rabbit Anti-NT5E Polyclonal Antibody, Western Blot (WB) – Cat# MBS242461

Other Notes

Small volumes of anti-NT5E antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for NT5E. It may not necessarily be applicable to this product.

NCBI GI #
4505467
NCBI GeneID
4907
NCBI Accession #
NP_002517.1
UniProt Primary Accession #
P21589
UniProt Secondary Accession #
O75520; Q5W116; B3KQI8
UniProt Related Accession #
P21589
GenBank Nucleotide #
NM_002526.3
NCBI Official Full Name
5'-nucleotidase isoform 1 preproprotein
NCBI Official Synonym Full Names
5'-nucleotidase, ecto (CD73)
NCBI Official Symbol
NT5E
NCBI Official Synonym Symbols
NT; eN; NT5; NTE; eNT; CD73; E5NT; CALJA
UniProt Protein Name
5'-nucleotidase
UniProt Synonym Protein Names
Ecto-5'-nucleotidase; CD_antigen: CD73
UniProt Gene Name
NT5E
UniProt Synonym Gene Names
NT5; NTE; 5'-NT
UniProt Entry Name
5NTD_HUMAN
NCBI Protein Information
5'-nucleotidase; 5'-NT; ecto-5'-nucleotidase; Purine 5-Prime-Nucleotidase

NCBI Summary for NT5E

The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2011]
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UniProt Comments for NT5E

NT5E: Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Homodimer; disulfide-linked. Belongs to the 5'-nucleotidase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, GPI anchor; Phosphatase (non-protein); Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; Nucleotide Metabolism - purine; Nucleotide Metabolism - pyrimidine; EC 3.1.3.5; Membrane protein, integral

Chromosomal Location of Human Ortholog: 6q14-q21

Cellular Component: cell surface; membrane; cytoplasm; plasma membrane

Molecular Function: 5'-nucleotidase activity; metal ion binding; nucleotide binding

Biological Process: pyrimidine nucleoside catabolic process; pyrimidine base metabolic process; dephosphorylation; nucleobase, nucleoside and nucleotide metabolic process; adenosine biosynthetic process; negative regulation of inflammatory response; purine nucleotide catabolic process; DNA metabolic process; purine base metabolic process; AMP catabolic process

Disease: Calcification Of Joints And Arteries
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Research Articles on NT5E

1. ecto-nucleotidases CD39 and CD73 are expressed in human endometrial tumors

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-NT5E antibody

 Reference Product  PubMed Publications
 ENPP1 antibody  >4 publications with NT5E and ENPP1
 ADA antibody  >2 publications with NT5E and ADA
 ENTPD1 antibody  >2 publications with NT5E and ENTPD1

Pathways associated with anti-NT5E antibody

 Products by Pathway  Pathway Diagram
 HIF-1-alpha Transcription Factor Network Pathway antibodies  HIF-1-alpha Transcription Factor Network Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Nucleotides Pathway antibodies  Metabolism Of Nucleotides Pathway Diagram
 Nicotinate And Nicotinamide Metabolism Pathway antibodies  Nicotinate And Nicotinamide Metabolism Pathway Diagram
 Nicotinate And Nicotinamide Metabolism Pathway antibodies  Nicotinate And Nicotinamide Metabolism Pathway Diagram
 Purine Catabolism Pathway antibodies  Purine Catabolism Pathway Diagram
 Purine Metabolism Pathway antibodies  Purine Metabolism Pathway Diagram
 Purine Metabolism Pathway antibodies  Purine Metabolism Pathway Diagram
 Purine Metabolism Pathway antibodies  Purine Metabolism Pathway Diagram

Diseases associated with anti-NT5E antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >17 publications with NT5E and Inflammation
 Necrosis Antibodies  >9 publications with NT5E and Necrosis
 Breast Neoplasms Antibodies  >9 publications with NT5E and Breast Neoplasms
 Nervous System Diseases Antibodies  >9 publications with NT5E and Nervous System Diseases
 Disease Models, Animal Antibodies  >8 publications with NT5E and Disease Models, Animal
 Pain Antibodies  >7 publications with NT5E and Pain
 Brain Diseases Antibodies  >5 publications with NT5E and Brain Diseases
 Kidney Diseases Antibodies  >4 publications with NT5E and Kidney Diseases
 Heart Diseases Antibodies  >4 publications with NT5E and Heart Diseases
 Acute Kidney Injury Antibodies  >3 publications with NT5E and Acute Kidney Injury

Organs/Tissues associated with anti-NT5E antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >33 publications with NT5E and Blood
 Vascular Antibodies  >20 publications with NT5E and Vascular
 Bone Antibodies  >13 publications with NT5E and Bone
 Brain Antibodies  >9 publications with NT5E and Brain
 Bone Marrow Antibodies  >8 publications with NT5E and Bone Marrow
 Muscle Antibodies  >8 publications with NT5E and Muscle
 Mouth Antibodies  >8 publications with NT5E and Mouth
 Kidney Antibodies  >6 publications with NT5E and Kidney
 Ganglia Antibodies  >6 publications with NT5E and Ganglia
 Embryonic Tissue Antibodies  >5 publications with NT5E and Embryonic Tissue
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