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MAP4K1 Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS242871

HPK1, a KHS protein kinase, is an upstream activator of the stress-activated protein kinase (SAPK) signaling pathway. The protein has a structure similar to other GCK (Group I) kinases and contains four consensus SH3 binding motifs, which have been shown to… More details

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Product Overview

Online Datasheet in PDF
Product Name
MAP4K1 / HPK1, Polyclonal Antibody
Full Product Name
Goat Polyclonal to Human MAP4K1 / HPK1
Product Gene Name
anti-MAP4K1 antibody
Product Synonym Gene Name
HPK1
Product Synonym Names
Anti-MAP4K1 / HPK1 Antibody (N-Terminus) IHC-plus; MAP4K1; HPK1; MEKKK 1; MEK kinase kinase 1; MHPK1; Human MAP4K1; HPK1
Clonality
Polyclonal
Host
Goat
Species Reactivity
Gorilla, Human
Predicted Reactivity: Gibbon, Monkey, Bat, Bovine, Dog, Pig (at least 90% immunogen sequence identity)
Form/Format
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Purity/Purification
Immunoaffinity Purified
Concentration
0.5 mg/ml (lot specific)
Specificity
Human MAP4K1. This antibody is expected to recognise isoform 1 (NP_001036065.1) and isoform 2 (NP_009112.1).
Preparation and Storage
Store at -20 degree C. Minimize freezing and thawing.
Molecular Weight
90,425 Da
OMIM
601983
3D Structure
ModBase 3D Structure for Q92918
Target Species
Human
Immunogen Description
Synthetic peptide DVVDPDIFNRDPR-C from the N-terminus of human MAP4K1 (NP_001036065.1; NP_009112.1). Percent identity by BLAST analysis: Human, Gorilla (100%); Gibbon, Monkey, Dog, Bat, Bovine, Pig (92%); Elephant, Panda, Horse (85%).
Immunogen Type
Synthetic peptide
Immunogen
MAP4K1 / HPK1 antibody was raised against synthetic peptide DVVDPDIFNRDPR-C from the N-terminus of human MAP4K1 (NP_001036065.1; NP_009112.1). Percent identity by BLAST analysis: Human, Gorilla (100%); Gibbon, Monkey, Dog, Bat, Bovine, Pig (92%); Elephant, Panda, Horse (85%).
Antigen Modification
N-Terminus

Product Categories/Family for anti-MAP4K1 antibody

Family: Protein Kinase; Subfamily: KHS
Applications Tested/Suitable for anti-MAP4K1 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes for anti-MAP4K1 antibody
ELISA, IHC-P (2.5 - 3.75 ug/ml), WB (0.5 - 2 ug/ml)

Immunohistochemistry (IHC) of anti-MAP4K1 antibody

Anti-MAP4K1 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.
Goat Anti-MAP4K1 Polyclonal Antibody – Cat# MBS242871

Western Blot (WB) of anti-MAP4K1 antibody

Staining (0.5 ug/ml) of Jurkat lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.
Goat Anti-MAP4K1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS242871

Other Notes

Small volumes of anti-MAP4K1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MAP4K1 purchase

MBS242871 is an antibody targeting MAP4K1. Also known as Goat Polyclonal to Human MAP4K1 / HPK1. Reactive in Gorilla, Human
Predicted Reactivity: Gibbon, Monkey, Bat, Bovine, Dog, Pig (at least 90% immunogen sequence identity). Host: Goat.

Additional Details

NCBI/Uniprot data below describe general gene information for MAP4K1. It may not necessarily be applicable to this product.

NCBI GI #
6005810
NCBI GeneID
11184
NCBI Accession #
NP_009112.1
UniProt Primary Accession #
Q92918
UniProt Related Accession #
Q92918
GenBank Nucleotide #
NM_007181.5
NCBI Official Full Name
mitogen-activated protein kinase kinase kinase kinase 1 isoform 2
NCBI Official Synonym Full Names
mitogen-activated protein kinase kinase kinase kinase 1
NCBI Official Symbol
MAP4K1
NCBI Official Synonym Symbols
HPK1
UniProt Protein Name
Mitogen-activated protein kinase kinase kinase kinase 1
UniProt Synonym Protein Names
Hematopoietic progenitor kinase; MAPK/ERK kinase kinase kinase 1; MEK kinase kinase 1; MEKKK 1
Protein Family
Mitogen-activated protein kinase kinase kinase kinase
UniProt Gene Name
MAP4K1
UniProt Synonym Gene Names
HPK1; MEK kinase kinase 1; MEKKK 1
UniProt Entry Name
M4K1_HUMAN
NCBI Protein Information
mitogen-activated protein kinase kinase kinase kinase 1; MEKKK 1; MEK kinase kinase 1; MAPK/ERK kinase kinase kinase 1; hematopoietic progenitor kinase 1

UniProt Comments for MAP4K1

HPK1: a protein kinase of the STE20 family. Expressed primarily in hematopoietic organs, including bone marrow, spleen and thymus. Also expressed at very low levels in lung, kidney, mammary glands and small intestine. May play a role in hematopoietic lineage decisions and growth regulation. Positively regulated by PGE2 and a negative regulator of PGE2-induced FOS gene transcription.

Protein type: Protein kinase, STE; Kinase, protein; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); STE group; STE20 family; KHS subfamily

Chromosomal Location of Human Ortholog: 19q13.1-q13.4

Cellular Component: membrane; cytoplasm

Molecular Function: protein serine/threonine kinase activity; protein binding; MAP kinase kinase kinase kinase activity; ATP binding; protein kinase activity

Biological Process: cell proliferation; peptidyl-serine phosphorylation; protein amino acid autophosphorylation; activation of MAPKKK activity; response to stress; protein amino acid phosphorylation; activation of JNK activity
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Research Articles on MAP4K1

1. CUL7/Fbxw8 ubiquitin ligase-mediated HPK1 degradation revealed a direct link and novel role of CUL7/Fbxw8 ubiquitin ligase in the MAPK pathway, which plays a critical role in cell proliferation and differentiation.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-MAP4K1 antibody

 Reference Product  PubMed Publications
 GRB2 antibody  >1 publications with MAP4K1 and GRB2
 CRKL antibody  >1 publications with MAP4K1 and CRKL
 CRK antibody  >1 publications with MAP4K1 and CRK

Pathways associated with anti-MAP4K1 antibody

 Products by Pathway  Pathway Diagram
 B Cell Receptor Signaling Pathway antibodies  B Cell Receptor Signaling Pathway Diagram
 BCR Signaling Pathway antibodies  BCR Signaling Pathway Diagram
 Insulin Signaling Pathway antibodies  Insulin Signaling Pathway Diagram
 JNK Signaling In The CD4+ TCR Pathway antibodies  JNK Signaling In The CD4+ TCR Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 Prostate Cancer Pathway antibodies  Prostate Cancer Pathway Diagram
 Signaling Events Mediated By Stem Cell Factor Receptor (c-Kit) Pathway antibodies  Signaling Events Mediated By Stem Cell Factor Receptor (c-Kit) Pathway Diagram
 Signaling Of Hepatocyte Growth Factor Receptor Pathway antibodies  Signaling Of Hepatocyte Growth Factor Receptor Pathway Diagram

Diseases associated with anti-MAP4K1 antibody

 Disease Name  Pubmed Publications
 Urinary Bladder Neoplasms Antibodies  >1 publications with MAP4K1 and Urinary Bladder Neoplasms

Organs/Tissues associated with anti-MAP4K1 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >2 publications with MAP4K1 and Blood
 Brain Antibodies  >1 publications with MAP4K1 and Brain
 Intestine Antibodies  >1 publications with MAP4K1 and Intestine
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