• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

APOF Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS243289

Apolipoprotein F (APOF) is one of the minor apolipoproteins found in plasma. APOF forms complexes with lipoproteins and may be involved in transport and/or esterification of cholesterol. More details

DISCONTINUED
Click here to view our similar product(s) OR click here to contact our technical support team for suitable replacement.
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Product Name
APOF / Apolipoprotein F, Polyclonal Antibody
Full Product Name
Goat Polyclonal to Human APOF / Apolipoprotein F
Product Gene Name
Product Synonym Names
Anti-APOF / Apolipoprotein F Antibody (C-Terminus) IHC-plus; APOF; Apo-F; LTIP; Apolipoprotein F; Human APOF; Apolipoprotein F
Clonality
Polyclonal
Host
Goat
Species Reactivity
Gibbon, Gorilla, Human, Orangutan
Predicted Reactivity: Monkey (at least 90% immunogen sequence identity)
Form/Format
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Purity/Purification
Immunoaffinity Purified
Concentration
0.5 mg/ml (lot specific)
Specificity
Human APOF / Apolipoprotein F.
Preparation and Storage
Store at -20 degree C. Minimize freezing and thawing.
Molecular Weight
35,399 Da
OMIM
107760
3D Structure
ModBase 3D Structure for Q13790
Target Species
Human
Immunogen Description
Synthetic peptide C-KSYDLDPGAGSLE from the C-terminus of human APOF / Apolipoprotein F (NP_001629.1). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon (100%); Monkey (92%); Marmoset (85%).
Immunogen Type
Synthetic peptide
Immunogen
APOF / Apolipoprotein F antibody was raised against synthetic peptide C-KSYDLDPGAGSLE from the C-terminus of human APOF / Apolipoprotein F (NP_001629.1). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon (100%); Monkey (92%); Marmoset (85%).
Antigen Modification
C-Terminus
Applications Tested/Suitable for anti-APOF antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes for anti-APOF antibody
ELISA (1:32000, 0.3 - 1 ug/ml), IHC-P (1.25 - 3.75 ug/ml), WB (0.3 - 1 ug/ml)
Usage: Peptide ELISA: antibody detection limit dilution 1:32000. Western Blot: Approx. 35kD band observed in Human Liver lysates (calculated MW of 35.4kD according to NP_001629.1). Recommended concentration: 0.3-1 ug/ml.

Immunohistochemistry (IHC) of anti-APOF antibody

Anti-APOF / Apolipoprotein F antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 1.25 ug/ml.
Goat Anti-APOF Polyclonal Antibody – Cat# MBS243289

Western Blot (WB) of anti-APOF antibody

Antibody (0.5 ug/ml) staining of Human Liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-APOF Polyclonal Antibody, Western Blot (WB) – Cat# MBS243289

Other Notes

Small volumes of anti-APOF antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for APOF purchase

MBS243289 is an antibody targeting APOF. Also known as Goat Polyclonal to Human APOF / Apolipoprotein F. Reactive in Gibbon, Gorilla, Human, Orangutan
Predicted Reactivity: Monkey (at least 90% immunogen sequence identity). Host: Goat.

Additional Details

NCBI/Uniprot data below describe general gene information for APOF. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
apolipoprotein F
NCBI Official Synonym Full Names
apolipoprotein F
NCBI Official Symbol
APOF
NCBI Official Synonym Symbols
LTIP; Apo-F
UniProt Protein Name
Apolipoprotein F
UniProt Synonym Protein Names
Lipid transfer inhibitor protein; LTIP
Protein Family
UniProt Gene Name
APOF
UniProt Synonym Gene Names
Apo-F; LTIP
UniProt Entry Name
APOF_HUMAN
NCBI Protein Information
apolipoprotein F; lipid transfer inhibitor protein

NCBI Summary for APOF

The product of this gene is one of the minor apolipoproteins found in plasma. This protein forms complexes with lipoproteins and may be involved in transport and/or esterification of cholesterol. [provided by RefSeq, Jul 2008]

UniProt Comments for APOF

APOF: Minor apolipoprotein that associates with LDL. Inhibits cholesteryl ester transfer protein (CETP) activity and appears to be an important regulator of cholesterol transport. Also associates to a lesser degree with VLDL, Apo-AI and Apo-AII.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 12q13.3

Cellular Component: extracellular region

Molecular Function: lipid transporter activity; cholesterol binding; receptor binding

Biological Process: cholesterol metabolic process; triacylglycerol metabolic process; cholesterol efflux; lipid metabolic process; lipid transport
Show more

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Organs/Tissues associated with anti-APOF antibody

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >12 publications with APOF and Liver
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000