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PIGY Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS243397

MBS243397 is an antibody targeting PIGY. Also known as Rabbit Polyclonal to Human PIGY. Reactive in Human, Mouse. Host: Rabbit. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
PIGY, Polyclonal Antibody
Full Product Name
Rabbit Polyclonal to Human PIGY
Product Gene Name
Product Synonym Names
Anti-PIGY Antibody (Internal) IHC-plus; PIGY; PIG-Y; PREY; Human PIGY
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
PBS containing 0.02% sodium azide
Purity/Purification
Immunoaffinity Purified
Concentration
1 mg/ml (lot specific)
Specificity
The PIG-Y gene encodes two proteins, one of which arises from leaky scanning of the mRNA. This antibody only detects isoform 1 of PIG-Y. Despite its predicted molecular weight, PIG-Y often migrates at 28-30 kD in SDS-PAGE.
Preparation and Storage
Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles. Store undiluted.
Molecular Weight
12,655 Da
3D Structure
ModBase 3D Structure for Q96I23
Target Species
Human
Immunogen Description
A 16 amino acid peptide near the center of human PIG-Y.
Immunogen Type
Synthetic peptide
Immunogen
PIGY antibody was raised against a 16 amino acid peptide near the center of human PIG-Y.
Antigen Modification
Internal
Applications Tested/Suitable for anti-PIGY antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes for anti-PIGY antibody
ELISA, IHC-P (5 ug/ml), WB (1 - 2 ug/ml)

Immunohistochemistry (IHC) of anti-PIGY antibody

Anti-PIGY antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Rabbit Anti-PIGY Polyclonal Antibody – Cat# MBS243397

Immunohistochemistry (IHC) of anti-PIGY antibody

Anti-PIGY antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Rabbit Anti-PIGY Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS243397

Immunohistochemistry (IHC) of anti-PIGY antibody

Anti-PIGY antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Rabbit Anti-PIGY Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS243397

Immunofluorescence (IF) of anti-PIGY antibody

Immunofluorescence of PIGY in human spleen tissue with PIGY1 antibody at 20 ug/ml.
Rabbit Anti-PIGY Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS243397

Western Blot (WB) of anti-PIGY antibody

Western blot of PIG-Y in human spleen tissue lysate with PIG-Y antibody at (A) 1 and (B) 2 ug/ml.
Rabbit Anti-PIGY Polyclonal Antibody, Western Blot (WB) – Cat# MBS243397

Other Notes

Small volumes of anti-PIGY antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for PIGY. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
protein preY, mitochondrial
NCBI Official Synonym Full Names
PIGY upstream reading frame
NCBI Official Symbol
PYURF
NCBI Official Synonym Symbols
PREY
UniProt Protein Name
Protein preY, mitochondrial
UniProt Synonym Protein Names
PIGY upstream reading frame protein
UniProt Gene Name
PYURF
UniProt Synonym Gene Names
PREY
UniProt Entry Name
PREY_HUMAN
NCBI Protein Information
protein preY, mitochondrial; PIGY upstream reading frame protein

NCBI Summary for PIGY

The product of this gene, which is well-conserved, is encoded by the same bicistronic transcript that encodes phosphatidylinositol glycan anchor biosynthesis, class Y, but the two proteins are unrelated. This gene represents the protein encoded by the upstream open reading frame, while the protein encoded by the downstream open reading frame is represented by GeneID:84992. [provided by RefSeq, Aug 2012]
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UniProt Comments for PIGY

PYURF: Belongs to the PREY family

Chromosomal Location of Human Ortholog: 4q

Cellular Component: endoplasmic reticulum membrane; glycosylphosphatidylinositol-N-acetylglucosaminyltransferase (GPI-GnT) complex; mitochondrion

Molecular Function: protein binding

Biological Process: positive regulation of metabolic process; GPI anchor biosynthetic process
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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