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ASCL3 Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS243630

MBS243630 is an antibody targeting ASCL3. Also known as Goat Polyclonal to Mouse ASCL3. Reactive in Human, Mouse, RatPredicted Reactivity: Gibbon, Monkey, Bat, Bovine, Dog, Hamster, Horse, Rabbit (at least 90% immunogen sequence identity). Host: Goat. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
ASCL3, Polyclonal Antibody
Full Product Name
Goat Polyclonal to Mouse ASCL3
Product Gene Name
Product Synonym Names
Anti-ASCL3 Antibody (Internal) IHC-plus; ASCL3; ASH-3; Achaete-scute homolog 3; HASH3; SGN1; BHLHa42; Mouse ASCL3
Clonality
Polyclonal
Host
Goat
Species Reactivity
Human, Mouse, Rat
Predicted Reactivity: Gibbon, Monkey, Bat, Bovine, Dog, Hamster, Horse, Rabbit (at least 90% immunogen sequence identity)
Form/Format
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Purity/Purification
Immunoaffinity Purified
Concentration
0.5 mg/ml (lot specific)
Specificity
Human ASCL3.
Preparation and Storage
Store at -20 degree C. Minimize freezing and thawing.
Molecular Weight
20,797 Da
OMIM
609154
3D Structure
ModBase 3D Structure for Q9NQ33
Target Species
Mouse
Immunogen Description
Synthetic peptide C-HLPEDYLEKRLSK from an internal region of human ASCL3 (NP_064435.1). Percent identity by BLAST analysis: Mouse, Rat (100%).
Immunogen Type
Synthetic peptide
Immunogen
ASCL3 antibody was raised against synthetic peptide C-HLPEDYLEKRLSK from an internal region of human ASCL3 (NP_064435.1). Percent identity by BLAST analysis: Mouse, Rat (100%); Human, Gibbon, Monkey, Marmoset, Hamster, Elephant, Panda, Dog, Bovine, Bat, Horse, Rabbit, Opossum, Platypus (92%); Pig, Turkey, Chicken, Lizard (85%).
Antigen Modification
Internal
Applications Tested/Suitable for anti-ASCL3 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes for anti-ASCL3 antibody
ELISA (1:8000), IHC-P (3.75 ug/ml), WB (0.1 - 0.3 ug/ml)
Usage: Peptide ELISA: antibody detection limit dilution 1:8000. Western blot: Approx 20kD band observed in Human and Mouse Liver lysates (calculated MW of 20.2kD according to NP_064435.1). Recommended concentration: 0.1-0.3 ug/ml.

Immunohistochemistry (IHC) of anti-ASCL3 antibody

Anti-ASCL3 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.
Goat Anti-ASCL3 Polyclonal Antibody – Cat# MBS243630

Western Blot (WB) of anti-ASCL3 antibody

ASCL3 antibody (0.1 ug/ml) staining of Human Liver lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-ASCL3 Polyclonal Antibody, Western Blot (WB) – Cat# MBS243630

Other Notes

Small volumes of anti-ASCL3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ASCL3. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
achaete-scute homolog 3
NCBI Official Synonym Full Names
achaete-scute family bHLH transcription factor 3
NCBI Official Symbol
ASCL3
NCBI Official Synonym Symbols
SGN1; HASH3; bHLHa42
UniProt Protein Name
Achaete-scute homolog 3
UniProt Synonym Protein Names
Class A basic helix-loop-helix protein 42; bHLHa42; bHLH transcriptional regulator Sgn-1
Protein Family
UniProt Gene Name
ASCL3
UniProt Synonym Gene Names
BHLHA42; HASH3; SGN1; ASH-3; hASH3; bHLHa42
UniProt Entry Name
ASCL3_HUMAN
NCBI Protein Information
achaete-scute homolog 3; achaete-scute complex homolog 3; bHLH transcriptional regulator Sgn-1; class A basic helix-loop-helix protein 42; bHLH transcription factor Sgn-1 (Salivary Glands 1)

NCBI Summary for ASCL3

Basic helix-loop-helix transcription factors, such as ASCL3, are essential for the determination of cell fate and the development and differentiation of numerous tissues (Jonsson et al., 2004 [PubMed 15475265]).[supplied by OMIM, Mar 2008]

UniProt Comments for ASCL3

ASCL3: Transcriptional repressor. Inhibits myogenesis.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 11p15.3

Cellular Component: transcription factor complex; nucleus

Molecular Function: protein dimerization activity; DNA binding

Biological Process: regulation of transcription from RNA polymerase II promoter; transcription, DNA-dependent
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with anti-ASCL3 antibody

 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >1 publications with ASCL3 and Carcinoma
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