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AXIN2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS243754

MBS243754 is an antibody targeting AXIN2. Also known as Rabbit Polyclonal to Human AXIN2 / Axin 2. Reactive in Human, Mouse, Rat. Host: Rabbit. More details

$650 / 0.05 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
AXIN2 / Axin 2, Polyclonal Antibody
Full Product Name
Rabbit Polyclonal to Human AXIN2 / Axin 2
Product Gene Name
anti-AXIN2 antibody
Product Synonym Names
Anti-AXIN2 / Axin 2 Antibody (C-Terminus) IHC-plus; AXIN2; AXIL; Axin-2; Axis inhibition protein 2; Axin 2; Axin-like protein; Conductin; Human AXIN2; Axin 2
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
PBS, 0.02% sodium azide
Purity/Purification
Peptide Column Affinity Chromatography
Concentration
1 mg/ml (lot specific)
Specificity
AXIN2 antibody is predicted to not cross-react with AXIN1. At least four isoforms of AXIN2 are known to exist; this antibody will recognize all four.
Preparation and Storage
Store at 4°C for up 3 months. Store at -20°C for up to 1 year. Avoid freeze/thaw cycles.
OMIM
gene 608615
3D Structure
ModBase 3D Structure for Q9Y2T1
Target
AXIN/ Axin 2
Immunogen Type
Synthetic peptide
Immunogen
AXIN2/Axin 2 antibody was raised againist a 20 AA synthetic peptide near the C-Terminus of human AXIN2. The immunogen is located within aa 780-830 of AXIN2.
Disclaimer
Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
Recommended Immunohistochemistry Protocol
The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:

Tissue Preparation:
Formalin fixation and embedding in paraffin wax.

Tissue Sectioning:
Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C.

Deparaffinization:
Wash dry slides in 3 changes of xylene - 5 minutes each @ RT

Rehydration:
Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT
Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT
Wash slides in 1 change of 80% alcohol - 3 minutes @ RT
Rinse slides in gentle running distilled water - 5 minutes @ RT

Antigen retrieval:
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) -1 minute @ RT

Immunostaining:
(Do not allow tissues to dry at any time during the staining procedure)
Apply a universal protein block - 20 minutes @ RT
Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT
Rinse slides in 1 X TBST - 1 minute @ RT
Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase streptavidin - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT
Wash slides in distilled water - 1 minute @ RT

Dehydrate:
(This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB)
Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT
Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT
Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT
Wash slides in 3 changes of xylene - 1 minute each @ RT
Apply coverslip
Applications Tested/Suitable for anti-AXIN2 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), Immunofluorescence (IF), ELISA (EIA)
Application Notes for anti-AXIN2 antibody
IHC-P: (5 µg/ml)
WB: (1 - 2 µg/ml)

AXIN2 antibody can be used for detection of AXIN2 by Western blot at 1 ug/mL. Antibody can also be used for immunohistochemistry starting at 5 ug/mL. For immunofluorescence start at 20 ug/mL. Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse and rat samples and Immunofluorescence in mouse and rat samples. All other applications and species not yet tested. Western Blot: 85, 93 kDa

Immunohistochemistry (IHC) of anti-AXIN2 antibody

Anti-AXIN2 / AXIL antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Rabbit Anti-AXIN2 Polyclonal Antibody – Cat# MBS243754

Immunohistochemistry (IHC) of anti-AXIN2 antibody

Anti-AXIN2 / AXIL antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Rabbit Anti-AXIN2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS243754

Immunofluorescence (IF) of anti-AXIN2 antibody

Immunofluorescence of AXIN2 in Rat Lung cells with AXIN2 antibody at 20 ug/ml.
Rabbit Anti-AXIN2 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS243754

Western Blot (WB) of anti-AXIN2 antibody

Western blot analysis of AXIN2 in mouse lung lysate with AXIN2 antibody at 1 ug/ml.
Rabbit Anti-AXIN2 Polyclonal Antibody, Western Blot (WB) – Cat# MBS243754

Other Notes

Small volumes of anti-AXIN2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for AXIN2. It may not necessarily be applicable to this product.

NCBI GI #
195927059
NCBI GeneID
8313
NCBI Accession #
NP_004646.3
UniProt Primary Accession #
Q9Y2T1
UniProt Secondary Accession #
Q3MJ88; Q9H3M6; Q9UH84
UniProt Related Accession #
Q9Y2T1
GenBank Nucleotide #
NM_004655.3
NCBI Official Full Name
axin-2
NCBI Official Synonym Full Names
axin 2
NCBI Official Symbol
AXIN2
NCBI Official Synonym Symbols
AXIL; ODCRCS
UniProt Protein Name
Axin-2
UniProt Synonym Protein Names
Axin-like protein; Axil; Axis inhibition protein 2; Conductin
Protein Family
Axin
UniProt Gene Name
AXIN2
UniProt Synonym Gene Names
Axil
UniProt Entry Name
AXIN2_HUMAN
NCBI Protein Information
axin-2; conductin; axin-like protein; axis inhibition protein 2

NCBI Summary for AXIN2

The Axin-related protein, Axin2, presumably plays an important role in the regulation of the stability of beta-catenin in the Wnt signaling pathway, like its rodent homologs, mouse conductin/rat axil. In mouse, conductin organizes a multiprotein complex of APC (adenomatous polyposis of the colon), beta-catenin, glycogen synthase kinase 3-beta, and conductin, which leads to the degradation of beta-catenin. Apparently, the deregulation of beta-catenin is an important event in the genesis of a number of malignancies. The AXIN2 gene has been mapped to 17q23-q24, a region that shows frequent loss of heterozygosity in breast cancer, neuroblastoma, and other tumors. Mutations in this gene have been associated with colorectal cancer with defective mismatch repair. [provided by RefSeq, Jul 2008]
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UniProt Comments for AXIN2

axin 2: is a negative regulator of the Wnt pathway, which is critical in stem cell signaling, morphogenesis, the mesenchymal-epithelial transition, and many cancers. Axin functions as a tumor suppressor. Probably facilitates the phosphorylation of beta-catenin and APC by GSK3B, leading to their ubiquitination and subsequent proteolysis. Is downregulated during progression of esophageal squamous cell carcinoma. Axin1/2 mediate cross-talk between TGF-beta and Wnt signaling pathways.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 17q24.1

Cellular Component: microtubule cytoskeleton; nucleoplasm; Golgi apparatus; centrosome; cytoplasmic microtubule; postsynaptic density; cytoplasmic membrane-bound vesicle; cytoplasm; plasma membrane; cell cortex; nucleus; beta-catenin destruction complex; cytosol

Molecular Function: protein binding; enzyme binding; ubiquitin protein ligase binding; beta-catenin binding; protein kinase binding; GTPase activator activity

Biological Process: odontogenesis; negative regulation of cell proliferation; cell proliferation; intramembranous ossification; regulation of mismatch repair; mRNA stabilization; negative regulation of osteoblast differentiation; dorsal/ventral axis specification; positive regulation of protein amino acid phosphorylation; maintenance of DNA repeat elements; bone mineralization; positive regulation of GTPase activity

Disease: Oligodontia-colorectal Cancer Syndrome
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Research Articles on AXIN2

1. findings indicate that AXIN2 can be regarded as a candidate gene for mutation detection in individuals with non-syndromic oligodontia in the Chinese population.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-AXIN2 antibody

 Reference Product  PubMed Publications
 CTNNB1 antibody  >313 publications with AXIN2 and CTNNB1
 APC antibody  >81 publications with AXIN2 and APC
 WNT3A antibody  >50 publications with AXIN2 and WNT3A
 AXIN1 antibody  >47 publications with AXIN2 and AXIN1
 LRP6 antibody  >23 publications with AXIN2 and LRP6
 TCF7L2 antibody  >18 publications with AXIN2 and TCF7L2
 GSK3B antibody  >2 publications with AXIN2 and GSK3B
 APC2 antibody  >1 publications with AXIN2 and APC2
 CSNK1E antibody  >1 publications with AXIN2 and CSNK1E

Pathways associated with anti-AXIN2 antibody

 Products by Pathway  Pathway Diagram
 Basal Cell Carcinoma Pathway antibodies  Basal Cell Carcinoma Pathway Diagram
 Basal Cell Carcinoma Pathway antibodies  Basal Cell Carcinoma Pathway Diagram
 Canonical Wnt Signaling Pathway antibodies  Canonical Wnt Signaling Pathway Diagram
 Colorectal Cancer Pathway antibodies  Colorectal Cancer Pathway Diagram
 Colorectal Cancer Pathway antibodies  Colorectal Cancer Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Endometrial Cancer Pathway antibodies  Endometrial Cancer Pathway Diagram
 Endometrial Cancer Pathway antibodies  Endometrial Cancer Pathway Diagram
 Hippo Signaling Pathway antibodies  Hippo Signaling Pathway Diagram
 Hippo Signaling Pathway antibodies  Hippo Signaling Pathway Diagram

Diseases associated with anti-AXIN2 antibody

 Disease Name  Pubmed Publications
 Colorectal Neoplasms Antibodies  >69 publications with AXIN2 and Colorectal Neoplasms
 Colonic Neoplasms Antibodies  >36 publications with AXIN2 and Colonic Neoplasms
 Disease Models, Animal Antibodies  >17 publications with AXIN2 and Disease Models, Animal
 Liver Diseases Antibodies  >16 publications with AXIN2 and Liver Diseases
 Liver Neoplasms Antibodies  >16 publications with AXIN2 and Liver Neoplasms
 Inflammation Antibodies  >10 publications with AXIN2 and Inflammation
 Fibrosis Antibodies  >10 publications with AXIN2 and Fibrosis
 Carcinoma, Hepatocellular Antibodies  >10 publications with AXIN2 and Carcinoma, Hepatocellular
 Neoplasms, Experimental Antibodies  >8 publications with AXIN2 and Neoplasms, Experimental
 Hyperplasia Antibodies  >7 publications with AXIN2 and Hyperplasia

Organs/Tissues associated with anti-AXIN2 antibody

 Organ/Tissue Name  Pubmed Publications
 Embryonic Tissue Antibodies  >29 publications with AXIN2 and Embryonic Tissue
 Blood Antibodies  >28 publications with AXIN2 and Blood
 Brain Antibodies  >27 publications with AXIN2 and Brain
 Liver Antibodies  >23 publications with AXIN2 and Liver
 Lung Antibodies  >21 publications with AXIN2 and Lung
 Intestine Antibodies  >18 publications with AXIN2 and Intestine
 Nerve Antibodies  >15 publications with AXIN2 and Nerve
 Kidney Antibodies  >12 publications with AXIN2 and Kidney
 Connective Tissue Antibodies  >12 publications with AXIN2 and Connective Tissue
 Eye Antibodies  >10 publications with AXIN2 and Eye
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